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Analysis of ciliary protein complex that determines renal cyst developments

Research Project

Project/Area Number 24790198
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General anatomy (including Histology/Embryology)
Research InstitutionJapan Aerospace Exploration Agency (2013)
Kyoto Prefectural University of Medicine (2012)

Principal Investigator

SHIBA DAI  独立行政法人宇宙航空研究開発機構, 有人宇宙ミッション本部, 主任研究員 (50360722)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords一次繊毛 / 腎臓 / 嚢胞 / メカノストレス / 前庭機能 / シグナル伝達 / 繊毛 / 分子構造 / 生体応答 / 核移行
Research Abstract

Primary cilia have diverse functions in cell signaling pathways, including mechanosensation and hedgehog signaling. More than 1000 proteins are now identified to localize in the cilium. Nephronophthisis (NPHP) is an autosomal recessive cystic kidney disease. Among 12 reported Nphp gene products, Inv/Nphp2 and Nek8/Nphp9 are localized to the proximal segment in the primary cilium. However, the functional relationships are unknown. This study focused on phenotype analysis of nek8 knockdown embryos and the genetic relationship between nek8 and inv in zebrafish. Knockdown of nek8 produced pronephric cysts. Simultaneous knockdown of nek8 and inv synergistically increased the incidence of these defects. Interestingly, nek8 mRNA rescued inv morphant phenotypes, although inv mRNA could not rescue nek8 morphant phenotypes. These results suggest that Nek8 acts downstream of Inv function.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] Deficiency of NOX1/nicotinamide adenine dinucleotide phosphate, reduced form oxidase leads to pulmonary vascular remodeling2014

    • Author(s)
      Iwata K, Ikami K, Matsuno K, Yamashita T, Shiba D, Ibi M, Matsumoto M, Katsuyama M, Cui W, Zhang J, Zhu K, Takei N, Kokai Y, Ohneda O, Yokoyama T, Yabe-Nishimura C
    • Journal Title

      Arterioscler Thromb Vasc Biol.

      Volume: 34(1) Pages: 110-9

    • Related Report
      2013 Final Research Report
  • [Journal Article] Deficiency of NOX1/nicotinamide adenine dinucleotide phosphate, reduced form oxidase leads to pulmonary vascular remodeling.2014

    • Author(s)
      Iwata K, Ikami K, Matsuno K, Yamashita T, Shiba D, Ibi M, Matsumoto M, Katsuyama M, Cui W, Zhang J, Zhu K, Takei N, Kokai Y, Ohneda O, Yokoyama T, Yabe-Nishimura C.
    • Journal Title

      Arterioscler Thromb Vasc Biol.

      Volume: 34(1) Issue: 1 Pages: 110-119

    • DOI

      10.1161/atvbaha.113.302107

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 宇宙マウスにかける夢2014

    • Author(s)
      芝 大
    • Journal Title

      生物工学会誌

      Volume: 第92巻1号 Pages: 44-44

    • Related Report
      2013 Annual Research Report
  • [Journal Article] The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp2 during pronephros morphogenesis and left-right establishment in zebrafish2012

    • Author(s)
      Fukui H, Shiba D, Asakawa K, Kawakami K and Yokoyama T
    • Journal Title

      FEBS Lett.

      Volume: 586(16) Pages: 2273-9

    • Related Report
      2013 Final Research Report
  • [Journal Article] The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp2 during pronephros morphogenesis and left-right establishment in zebrafish.2012

    • Author(s)
      Fukui H, Shiba D, Asakawa K, Kawakami K and Yokoyama T.
    • Journal Title

      FEBS Lett.

      Volume: 586(16) Pages: 2273-2279

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp22013

    • Author(s)
      芝大、福井一、横山尚彦
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      香川
    • Related Report
      2013 Final Research Report
  • [Presentation] An attempt to isolate novel ciliary genes using whole mount in situ hybridization expression database2013

    • Author(s)
      小林大介,芝大,横山尚彦
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      香川
    • Related Report
      2013 Final Research Report
  • [Presentation] The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp22013

    • Author(s)
      Shiba D, Fukui H and Yokoyama T.
    • Organizer
      第118回日本解剖学会
    • Place of Presentation
      香川
    • Related Report
      2012 Research-status Report
  • [Presentation] A three-step process of Nphp3 ciliary localization2012

    • Author(s)
      Dai Shiba, Kana Nakata, Hajime Fukui, Daisuke Kobayashi and Takahiko Yokoyama
    • Organizer
      CILIA 2012-1st International Scientific Conference
    • Place of Presentation
      London(抄録CILIA 2012 Conference Book, 44)
    • Related Report
      2013 Final Research Report
  • [Presentation] The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp22012

    • Author(s)
      Dai Shiba, Hajime Fukui, Kazuhide Asakawa, Koichi Kawakam and Takahiko Yokoyama
    • Organizer
      The American Society for Cell Biology Annual Meeting
    • Place of Presentation
      The Moscone Center, San Francisco, CA(抄録The American Society for Cell Biology, Annual Meeting Program, L9)
    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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