• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of molecular mechanisms underlying regulation of basal activity of t-tubular L-type Ca channels in ventricular myocytes

Research Project

Project/Area Number 24790210
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionShinshu University

Principal Investigator

KASHIHARA Toshihide  信州大学, 医学部, 助教 (20552334)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsL型カルシウムチャネル / 心筋 / タンパク質リン酸化酵素 / 心不全 / T管 / 興奮収縮連関 / プロテインホスファターゼ2A / カゼインキナーゼ2
Research Abstract

Despite recently advanced medical therapies, heart failure is one of the leading risks of death in developed nations. T-tubular L-type Ca2+ channels (LTCC) in ventricular myocytes play a crucial role in cardiac excitation-contraction coupling. Decreased basal activity of t-tubular LTCC is involved in cardiac disfunction. We recently found that basal activity of LTCC is supported by casein kinase 2 (CK2). Here, I examined the molecular mechanisms underlying regulation of basal LTCC activity by CK2. In the result, I found that CK2a'b determines the basal activity of cardiac LTCC.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (23 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (17 results)

  • [Journal Article] β2-Adrenergic and M2-muscarinic receptors decrease basal t-tubular L-type Ca2+ channel activity and suppress ventricular contractility in heart failure.2014

    • Author(s)
      Kashihara T, Hongo M, et al.
    • Journal Title

      European Journal of Pharmacology

      Volume: 724 Pages: 122-131

    • DOI

      10.1016/j.ejphar.2013.12.037

    • NAID

      120007110580

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] TRPM4 channels mediate hypertonicity-induced, Ca2+-impermeable, non-selective cation currents in a cervical cancer cell line, HeLa cells2014

    • Author(s)
      Gomi S., Nakada T., Kashihara T., Ikeda U., Yamada M.
    • Journal Title

      Shinshu Medical Journal

      Volume: 62 Pages: 33-44

    • NAID

      130004551913

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel regulation pf cardiac metabolism and homeostasis by the adrenomedullin- receptor activity-modifying protein 2 system2013

    • Author(s)
      Takahiro Yoshizawa, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Yasuhiro Iesato, Teruhide Koyama, Ryuichi Uetake, Lei Yang, Akihiro Yamauchi, Megumu Tanaka, Yuichi Toriyama, Kyoko Igarashi, Tsutomu Nakada, Toshihide Kashihara, Mitsuhiko Yamada, Hayato Kawakami, Hiroki Nakanishi, Ryo Taguchi, Tsuyoshi Nakanishi, Hiroshi Akazawa, Takayuki Shindo
    • Journal Title

      Hypertension

      Volume: 61 Issue: 2 Pages: 341-351

    • DOI

      10.1161/hypertensionaha.111.00647

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nicorandil prevents Gαq-induced progressive heart failure and ventricular arrhythmias in transgenic mice2012

    • Author(s)
      Hirose M, Takeishi Y, Nakada T, Shimojo H, Kashihara T, Nishio A, Suzuki S, Mende U, Matsumoto K, Matsushita N, Taira E, Sato F, Yamada M
    • Journal Title

      PLoS One

      Volume: 7 Issue: 12 Pages: e52667-e52667

    • DOI

      10.1371/journal.pone.0052667

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The proximal C-terminus of α1C subunits is necessary for junctional membrane targeting of cardiac L-type calcium channels.2012

    • Author(s)
      Nakada T., Flucher E. B., Kashihara T., Sheng X., Shibazaki T., Horiuchi-Hirose M., Gomi S., Hirose M., Yamada M.
    • Journal Title

      The Biochemical journal

      Volume: 448 Issue: 2 Pages: 221-231

    • DOI

      10.1042/bj20120773

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two mechanistically distinct effects of dihydropyridine nifedipine on CaV1.2 L-type Ca2+ channels revealed by Timothy syndrome mutation2012

    • Author(s)
      Sheng X, Nakada T, Kobayashi M, Kashihara T, Shibazaki T, Horiuchi-Hirose M, Gomi S, Hirose M, Aoyama T, Yamada M.
    • Journal Title

      European journal of pharmacology

      Volume: 685 Issue: 1-3 Pages: 15-23

    • DOI

      10.1016/j.ejphar.2012.04.029

    • NAID

      120007110525

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Casein Kinase 2α'βDetermines the Basal Activity of Ca_V1.2 Cardiac L-type Ca^<2+> Channels2014

    • Author(s)
      Toshihide Kashihara, Gomi Shimmon, Mitsuhiko Yamada
    • Organizer
      第78回日本循環器学会学術集会
    • Place of Presentation
      日東京国際会議場
    • Year and Date
      2014-03-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of the basal activity of Ca_V1.2 cardiac L-type Ca^<2+> channels by casein kinase 2α'β2014

    • Author(s)
      Toshihide Kashihara, Tsutomu Nakada, Mitsuhiko Yamada
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of the basal activity of CaV1.2 cardiac L-type Ca2+ channels by casein kinase 2 α’β2014

    • Author(s)
      Toshihide Kashihara, Tsutomu Nakada, Mitsuhiko Yamada
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      東北大学百周年記念会館(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Casein Kinase 2α’β Determines the Basal Activity of CaV1.2 Cardiac L-type Ca2+ Channels2014

    • Author(s)
      Toshihide Kashihara, Gomi Shimmon, Mitsuhiko Yamada
    • Organizer
      第78回日本循環器学会学術集会
    • Place of Presentation
      東京国際フォーラム(東京都千代田区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] カゼインキナーゼ2による心筋Ca_V1.2L型Ca^<2+>チャネルの基礎活性制御の分子機構の解析2013

    • Author(s)
      柏原俊英、中田勉、山田充彦
    • Organizer
      第23回日本循環薬理学会
    • Place of Presentation
      福岡大学
    • Year and Date
      2013-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] カゼインキナーゼ2による心筋型L型Ca^<2+>チャネルの基礎活性制御の分子機構2013

    • Author(s)
      柏原俊英、中田勉、五味志文、山田充彦
    • Organizer
      第30回日本心電学会学術集会
    • Place of Presentation
      リンクステーションホール青森
    • Year and Date
      2013-10-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 慢性的で過剰なβ_1アドレナリン受容体刺激が心室筋の興奮収縮連関を修飾する分子機序について2013

    • Author(s)
      柏原俊英、中田勉、五味志文、弘瀬雅教、山田充彦
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] カゼインキナーゼ2によるCa_V1.2心筋型L型Ca^<2+>チャネルの基礎活性の制御2013

    • Author(s)
      柏原俊英、中田勉、五味志文、山田充彦
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] CaチャネルアゴニストFPL64176の作用機序の検討2013

    • Author(s)
      五味志文、柏原俊英、中田勉、池田宇一、山田充彦
    • Organizer
      第129回日本薬理学会関東部会
    • Place of Presentation
      順天堂大学本郷キャンパス(東京都文京区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] カゼインキナーゼ2による心筋型L型Ca2+チャネルの基礎活性制御の分子機構2013

    • Author(s)
      柏原俊英、中田勉、五味志文、山田充彦
    • Organizer
      第30回日本心電学会学術集会
    • Place of Presentation
      リンクステーション青森(青森県弘前市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] カゼインキナーゼ2による心筋CaV1.2L型Ca2+チャネルの基礎活性制御の分子機構の解析2013

    • Author(s)
      柏原俊英、中田勉、山田充彦
    • Organizer
      第23回日本循環薬理学会
    • Place of Presentation
      福岡大学メディカルホール(福岡県福岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] スタウロスポリン感受性キナーゼとカゼインキナーゼ2による心室筋L型Caチャネルの基礎活性の制御2012

    • Author(s)
      柏原俊英、中田勉、五味志文、山田充彦
    • Organizer
      第29回日本心電学会学術集会
    • Place of Presentation
      幕張メッセ国際会議場
    • Year and Date
      2012-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] スタウロスポリン感受性キナーゼとカゼインキナーゼ2による心室筋L型Caチャネルの基礎活性の制御

    • Author(s)
      柏原俊英、中田勉、五味志文、山田充彦
    • Organizer
      第29回日本心電学会学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
  • [Presentation] カゼインキナーゼ2によるCaV1.2心筋型L型Ca2+チャネルの基礎活性の制御

    • Author(s)
      柏原俊英、中田勉、五味志文、山田充彦
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] L型カルシウムチャネルおよびリアノジン受容体の骨格筋細胞内局在と機能に対するジャンクトフィリンの発現抑制の影響

    • Author(s)
      中田勉、ブルナルド フルハー、柏原俊英、五味志文、山田充彦
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 慢性的で過剰なβ1アドレナリン受容体刺激が心室筋の興奮収縮連関を修飾する分子機序について

    • Author(s)
      柏原俊英、中田勉、五味志文、弘瀬雅教、山田充彦
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 骨格筋のL型カルシウムチャネルとリアノジン受容体の局在・機能に対するジャンクトフィリンの発現抑制の影響

    • Author(s)
      中田勉、ブルナルド フルハー、柏原俊英、五味志文、山田充彦
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi