The regulation of cytosolic chloride ion concentration which regulates autophagy system via regulation of lysosome acidity is a new target for cancer therapy
| Project/Area Number |
24790220
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HOSOGI SHIGEKUNI 京都府立医科大学, 医学(系)研究科(研究院), 助教 (30433254)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | クロライドイオン / オートファジー / リソソーム / autophagy / chloride ion |
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| Research Abstract |
Autophagy is a catabolic process degrading cell components mediated through lysosomal machineries. Lysosome is, therefore, a key organelle in autophagy degrading various compounds. The digesting activity of lysosomal enzymes depends on intra-lysosomal acidity. we clarified roles of cytosolic chloride ion in regulation of lysosomal acidification intra-lysosomal pH and autophagy function in human cancer cell lines and mouse embryonic fibroblast cells. The lowered [Cl-]c primarily causes dysfunction of autophagy via dysfunction of lysosome induced by disturbance of intra-lysosomal acidification.
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Report
(3 results)
Research Products
(6 results)
