The regulation of cytosolic chloride ion concentration which regulates autophagy system via regulation of lysosome acidity is a new target for cancer therapy

Research Project

Project/Area Number	24790220
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General physiology
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	HOSOGI SHIGEKUNI 京都府立医科大学, 医学(系)研究科(研究院), 助教 (30433254)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	クロライドイオン / オートファジー / リソソーム / autophagy / chloride ion
Research Abstract	Autophagy is a catabolic process degrading cell components mediated through lysosomal machineries. Lysosome is, therefore, a key organelle in autophagy degrading various compounds. The digesting activity of lysosomal enzymes depends on intra-lysosomal acidity. we clarified roles of cytosolic chloride ion in regulation of lysosomal acidification intra-lysosomal pH and autophagy function in human cancer cell lines and mouse embryonic fibroblast cells. The lowered [Cl-]c primarily causes dysfunction of autophagy via dysfunction of lysosome induced by disturbance of intra-lysosomal acidification.

Report

(3 results)