| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, adenosine regulates transepithelial anion secretion in duct cells and is considered to play a role in acini-to-duct signaling. To identify the functional adenosine receptors and Cl channels important for anion secretion, we herein performed experiments on pancreatic duct cells using electrophysiological techniques. The addition of adenosine increased the transepitheial ion transport and whole-cell Cl currents in duct cells. Immunohistochemical analysis showed that the A2B receptors localized in the luminal membranes of the pancreatic ducts. These results indicate that luminal adenosine regulates anion secretion by activating cAMP-activated Cl channels via adenosine A2B receptors on the luminal membranes of the pancreatic duct cells.
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