The mechanism of the function and physiological role of TASK channel in adrenaline secretion
| Project/Area Number |
24790228
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | University of Occupational and Environmental Health, Japan |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | TASKチャネル / エンドサイトーシス / TASK1チャネル / Src / PKC |
|---|
| Research Abstract |
I investigated the molecular mechanism of regulation of TASK1 channels in endocrine cells. Pharmacological and molecular biological analyses showed that TASK1 channels are regulated by the clathrin-dependent endocytosis in NGF-stimulated adrenal medullary and PC12 cells. Mutation analysis of the TASK1 channel revealed that the dileucine motif was involved in at least part of the endocytosis. Furthermore, I showed that Src, PLC and PI3K are involved in NGF-induced endocytosis of TASK1 channels in PC12 cells. Additionally, the expression of TASK1 channels at the protein and mRNA levels was suppressed in PC12 cells treated with NGF for 2 weeks. These results indicate that NGF suppresses the expression of TASK1 channels in the plasma membrane via not only endocytosis but also the inhibition of gene transcription.
|
Report
(3 results)
Research Products
(9 results)
