• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanisms of HGF-beta-induced macrophage phagocytosis

Research Project

Project/Area Number 24790250
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General pharmacology
Research InstitutionKinjo Gakuin University

Principal Investigator

OHNISHI Hiroyuki  金城学院大学, 薬学部, 助教 (90523316)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords肝細胞増殖因子 / マンノース受容体 / マクロファージ / 貪食作用
Research Abstract

We provide evidence that hepatocyte growth factor (HGF) beta-chain remnant (HGF-beta), generated through fragmentation of HGF by proteases released from inflammatory cells such as neutrophils, potently stimulated phagocytosis of apoptotic neutrophils by binding to mannose receptor (MR), which recently identified as an HGF-beta receptor. Upon binding to MR, HGF-beta may modulate actin cytoskeleton and enhance phagocytosis through interaction between MR and actin-binding protein IQGAP1. HGF-beta also induced surface expression of CD11b, a component of receptor complex required for complement-mediated phagocytosis. These findings suggest that HGF-beta/MR axis-induced macrophage phagocytosis could prevent accumulation of apoptotic neutrophils and release of inflammatory molecules from neutrophils, which leads to resolution of inflammation and promotion of tissue repair.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (6 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Dissociation of c-Met phosphotyrosine sites in human cells in response to mouse hepatocyte growth factor but not human hepatocyte growth factor : the possible roles of different amino acids in different species2013

    • Author(s)
      Ikebuchi F, Oka K, Mizuno S, Fukuta K, Hayata D, Ohnishi H, Nakamura T
    • Journal Title

      Cell Biochem. Funct

      Volume: 31 Pages: 298-304

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dissociation of c-Met phospho-tyrosine sites in human cells in response to mouse HGF, but not human HGF: The possible roles of different amino acids in different species.2013

    • Author(s)
      Ikebuchi F, Oka K, Mizuno S, Fukuta K, Hayata D, Ohnishi H and Nakamura T:
    • Journal Title

      Cell Biochem Function

      Volume: 31 Issue: 4 Pages: 298-304

    • DOI

      10.1002/cbf.2898

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of mannose receptor as receptor for hepatocyte growth factorβ-chain : novel ligand-receptor pathway for enhancing macrophage phagocytosis2012

    • Author(s)
      Ohnishi H, Oka K, Mizuno S, Nakamura T
    • Journal Title

      J. Biol. Chem

      Volume: 287 Pages: 13371-1381

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of mannose receptor as a receptor for HGFβ-chain : A novel ligand-receptor pathway for enhancing macrophage phagocytosis2012

    • Author(s)
      Ohnishi H, Oka K, Mizuno S and NakamuraT.
    • Journal Title

      J. Biol. Chem

      Volume: 287 Issue: 16 Pages: 13371-13381

    • DOI

      10.1074/jbc.m111.318568

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 炎症性プロテアーゼによるHGF断片化で生じたHGFβ鎖の生物活性2013

    • Author(s)
      大西浩之
    • Organizer
      第137回薬学談話会
    • Place of Presentation
      名古屋市立大学(愛知県)
    • Year and Date
      2013-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 炎症性プロテアーゼによるHGF断片化で生じたHGFβ鎖の生物活性2013

    • Author(s)
      大西浩之
    • Organizer
      名古屋市立大学 第137回薬学談話会
    • Place of Presentation
      名古屋市立大学薬学部
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] HGFβ-chain受容体としてのマンノースレセプターの同定と新規貪食促進機構の可能性2012

    • Author(s)
      大西浩之, 岡清正, 水野信哉, 中村敏一
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県)
    • Year and Date
      2012-12-15
    • Related Report
      2013 Final Research Report
  • [Presentation] HGFβ-chain受容体としてのマンノース受容体の同定2012

    • Author(s)
      大西浩之, 岡清正, 水野信哉, 中村敏一
    • Organizer
      第58回日本薬学会東海支部会
    • Place of Presentation
      静岡県立大学(静岡県)
    • Year and Date
      2012-07-07
    • Related Report
      2013 Final Research Report
  • [Presentation] HGF β-chain受容体としてのマンノース受容体の同定2012

    • Author(s)
      大西浩之、岡清正、水野信哉、中村敏一
    • Organizer
      第58回日本薬学会東海支部会
    • Place of Presentation
      静岡
    • Related Report
      2012 Research-status Report
  • [Presentation] HGF-β受容体としてのマンノースレセプターの同定と新規貪食促進機構の可能性2012

    • Author(s)
      大西浩之、岡清正、水野信哉、中村敏一
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Book] Physiological Roles and Therapeutic Implications of Hepatocyte Growth Factor for Angiogenesis. In"Biochemical Basis and Therapeutic Implications of Angiogenesis"2013

    • Author(s)
      Ohnishi H, Oka K, Mizuno S, Nakamura T
    • Publisher
      Springer Science + Business Media New York
    • Related Report
      2013 Final Research Report
  • [Book] Physiological Roles and Therapeutic Implications of Hepatocyte Growth Factor for Angiogenesis2013

    • Author(s)
      Ohnishi H, Oka K, Mizuno S, Nakamura T.
    • Total Pages
      30
    • Publisher
      Springer Science+Business Media New York. (印刷中)
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi