Analysis of protein Cysteome modification in G protein-coupled receptor
| Project/Area Number |
24790252
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Kobe University |
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Principal Investigator |
ADACHI Naoko 神戸大学, バイオシグナル研究センター, 助教 (70604510)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITO Naoaki 神戸大学, バイオシグナル研究センター, 教授 (60178499)
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| Research Collaborator |
KAKU Mika 神戸大学, 大学院医学研究科, M2
UEDA Chie 神戸大学, バイオシグナル研究センター, 技術補佐員
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| Project Period (FY) |
2013-02-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | パルミトイル化 / ベータ３アドレナリン受容体 / ニトロシル化 |
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| Outline of Final Research Achievements |
We have identified three palmitoylation sites of the human beta3-adrenergic receptor. Palmitoylation deficient mutants showed neither altered cAMP production nor receptor mislocalization. However, an irreversible palmitoylation inhibitor, 2-bromopalmitate greatly reduced receptor number on the plasma membrane. This result indicates that these palmitoylation are important for receptor stability. Since activation of beta3-adrenergic receptor facilitates lipid metabolism in fat cells therefore, protection of palmitoylated receptors might be a potential drug target against obesity.
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Report
(3 results)
Research Products
(3 results)
