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Interaction analysis of the blood-brain barrier (BBB) constitution cell in the experimental cerebral ischemia model

Research Project

Project/Area Number 24790258
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General pharmacology
Research InstitutionNagasaki University

Principal Investigator

NAKAGAWA Shinsuke  長崎大学, 医歯(薬)学総合研究科, 講師 (10404211)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords血液脳関門 / 虚血再還流 / 高血糖 / トランスフォーミング増殖因子-β / シロスタゾール / ペリサイト / Neurovascular Unit / Neurovascular unit
Research Abstract

The blood-brain barrier (BBB), a member of the neurovascular unit (NVU), functions in communication between brain capillary endothelium and other neighboring cells such as astrocytes and pericytes. BBB dysfunction plays a crucial role in brain edema formation and central nervous system disorders. Therefore, the experimental BBB dysfunction models under the pathological condition are applicable to search the protective agents against BBB dysfunction.
We have developed and characterized a new in vitro BBB model (BBB Kit) using primary cultures of the three main BBB-related cells obtained from rat brains. We attempted to make three types of BBB dysfunction models and succeeded in making following models: (i) hypoxia/reoxygenation model, (ii) hyperglycemia model, (iii) AGE-related ischemia model. We found that cilostazol protected the BBB against AGE-related ischemia, through an inhibitory mechanism on TGF-beta signaling.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 4 results) Presentation (14 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells.2014

    • Author(s)
      Yamada N, Nakagawa S, Horai S, Tanaka K, Deli MA, Yatsuhashi H, and Niwa M.
    • Journal Title

      Microvascular Research

      Volume: 92 Pages: 41-49

    • DOI

      10.1016/j.mvr.2013.12.004

    • NAID

      120006986529

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cilostazol attenuates ischemia-reperfusion-induced blood-brain barrier dysfunction enhanced by advanced glycation endproducts via transforming growth factor-β1 signaling.2014

    • Author(s)
      Takeshita T, Nakagawa S, Tatsumi R, So G, Hayashi K, Tanaka K, Deli MA, Nagata I, and Niwa M.
    • Journal Title

      Molecular and Cellular Neurosciences

      Volume: 60 Pages: 1-9

    • DOI

      10.1016/j.mcn.2014.01.006

    • NAID

      120006986528

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 血液脳関門(BBB)構成細胞の単離とin vitro BBBモデルの再構築2014

    • Author(s)
      中川慎介, 丹羽正美
    • Journal Title

      日本薬理学雑誌

      Volume: 143 Pages: 137-143

    • Related Report
      2013 Final Research Report
  • [Journal Article] 血液脳関門(BBB)構成細胞の単離と in vitro BBBモデルの再構築2014

    • Author(s)
      中川慎介、丹羽正美
    • Journal Title

      日本薬理学会雑誌

      Volume: 143 Pages: 137-143

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 血液脳関門in vitro再構成モデル(BBBキット)と機能解明への活用2013

    • Author(s)
      中川慎介, Mária A. Deli, Dinh Ha Duy Thuy, 相良真由美, 田中邦彦, 丹羽正美
    • Journal Title

      脳循環代謝

      Volume: 24 Pages: 71-74

    • Related Report
      2013 Final Research Report
  • [Journal Article] 脳内移行型薬剤開発のための血液脳関門in vitro再構成モデル(BBBキット)の開発2013

    • Author(s)
      中川慎介, Mária A. Deli, 巽理恵, 田中邦彦, 丹羽正美
    • Journal Title

      長崎市医師会報

      Volume: 47(7) Pages: 13-16

    • Related Report
      2013 Final Research Report
  • [Journal Article] Cilosatzol strengthens barrier integrity in brain endothelial cells2013

    • Author(s)
      Shoji Horai
    • Journal Title

      Cellular and Molecular Neurobiology

      Volume: 33 Issue: 2 Pages: 291-307

    • DOI

      10.1007/s10571-012-9896-1

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 血液脳関門 in vitro 再構成モデル(BBB キット)と機能解明への活用2013

    • Author(s)
      中川慎介、Maria A. Deli、Dinh Ha Duy Thuy、相良真由美、田中邦彦、 丹羽正美
    • Journal Title

      日本脳循環代謝学会機関誌

      Volume: 24 Pages: 71-74

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 脳内移行型薬剤開発のための血液脳関門 in vitro 再構成モデル(BBB キット)の開発2013

    • Author(s)
      中川慎介, Maria A. Deli, 巽理恵, 田中邦彦, 丹羽正美
    • Journal Title

      長崎市医師会報

      Volume: 47 Pages: 13-16

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Comparison of brain capillary endothelial cell-based and epithelial (MDCK-MDR1,Caco-2 and VB-Caco-2) cell-based surrogate blood-brain barrier penetration models2012

    • Author(s)
      Eva Hellinger
    • Journal Title

      Eurpean Jounal of Pharmaceutics and Biopharmaceutocs

      Volume: 82 Issue: 2 Pages: 340-351

    • DOI

      10.1016/j.ejpb.2012.07.020

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] In vitro reconstituted blood-brain barrier model and their application to drug discovery2014

    • Author(s)
      Shinsuke Nakagawa
    • Organizer
      The 1st international symposium Dejima challenge for therapeutic innovation
    • Place of Presentation
      Nagasaki, Japan
    • Year and Date
      2014-03-14
    • Related Report
      2013 Final Research Report
  • [Presentation] HGFとTGF-betaの血液能関門への作用比較(ポスター)2014

    • Author(s)
      中川慎介, 竹下朝規, 山田成美, 丹羽正美
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台市、仙台国際センター
    • Related Report
      2013 Final Research Report
  • [Presentation] In vitro reconstituted blood-brain barrier model and their application to drug discovery2014

    • Author(s)
      中川慎介
    • Organizer
      The 1st international symposium Dejima challenge for therapeutic innovation
    • Place of Presentation
      長崎市、長崎大学文教スカイホール
    • Related Report
      2013 Annual Research Report
  • [Presentation] HGFとTGF-betaの血液能関門への作用比較2014

    • Author(s)
      中川慎介、山田成美、竹下朝規、丹羽正美
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台市、仙台国際センター
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cilostazol improves the BBB barrier functions against AGEs-related ischemic damage via TGF-βsignaling2013

    • Author(s)
      Shuji Fukuda, Shinsuke Nakagawa, Tomonori Takeshita, Kentaro Hayashi, Rie Tatsumi, Tanaka Kunihiko, Maria A. Deli, Izumi Nagata, Masami Niwa
    • Organizer
      The 16th international symposium on "Signaling in the Blood-Brain Barriers"
    • Place of Presentation
      Sümeg, Hungary
    • Related Report
      2013 Final Research Report
  • [Presentation] 実験的糖尿病性脳虚血障害に対するシロスタゾールの保護作用とTGF-βシグナルの関与(ポスター)2013

    • Author(s)
      中川慎介, 竹下朝規, 巽理恵, Thuy DHD, 林健太郎, 田中邦彦, 永田泉, 丹羽正美
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡市、福岡国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] Cilostazol improves the BBB barrier functions against AGEs-related ischemic damage via TGF-β signaling2013

    • Author(s)
      福田修二、中川慎介、竹下朝規、林健太郎、巽理恵、田中邦彦、Maria A. Deli、永田泉、丹羽正美
    • Organizer
      The 16th international symposium on signaling in the Blood-Brain Barriers
    • Place of Presentation
      Sumeg, Hungary, Hotel Kapitany
    • Related Report
      2013 Annual Research Report
  • [Presentation] 実験的糖尿病性脳虚血障害に対するシロスタゾールの保護作用とTGF-βシグナルの関与2013

    • Author(s)
      中川慎介 他7名
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡国際会議場 (福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 血液脳関門In vitro再構成モデル(BBBキット)と機能解明への活用(シンポジスト)2012

    • Author(s)
      中川慎介, Maria A. Deli, Dinh Ha Duy Thuy, 相良真由美, 田中邦彦, 丹羽正美
    • Organizer
      第24回日本脳循環代謝学会総会
    • Place of Presentation
      広島市、リーガロイヤルホテル広島
    • Related Report
      2013 Final Research Report
  • [Presentation] 血液脳関門In vitro再構成モデル(BBBキット)(シンポジスト)2012

    • Author(s)
      中川慎介, Mária A. Deli, 巽理恵, Dinh Ha Duy Thuy, 相良真由美, 田中邦彦, 丹羽正美
    • Organizer
      第14回応用薬理シンポジウム
    • Place of Presentation
      甲府市、ベルクラシック甲府
    • Related Report
      2013 Final Research Report
  • [Presentation] In vitro血液脳関門再構成モデル(BBBキット)を用いたBBB機能の解析2012

    • Author(s)
      中川慎介, 丹羽正
    • Organizer
      第5回Japanese Consortium for Age-related Neurodegenerative disorders(J-CAN 2012)
    • Place of Presentation
      東京都中央区、八重洲ファーストファイナンシャルビル
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] In vitro 血液脳関門再構成モデル(BBBキット)を用いたBBB機能の解析2012

    • Author(s)
      中川慎介、丹羽正美
    • Organizer
      第5回Japanese Consortium for Age-related Neurodegenerative disorders(J-CAN 2012)
    • Place of Presentation
      八重洲ファーストフィナンシャルビル(東京都)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 血液脳関門 In vitro 再構成モデル(BBB キット)2012

    • Author(s)
      中川慎介 他5名
    • Organizer
      第14回応用薬理シンポジウム
    • Place of Presentation
      ベルクラシック甲府(山梨県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 中川慎介 他5名2012

    • Author(s)
      血液脳関門 In vitro 再構成モデル(BBB キット)と機能解明への活用
    • Organizer
      第24回日本脳循環代謝学会総会
    • Place of Presentation
      リーガロイヤルホテル広島 (広島県)
    • Related Report
      2012 Research-status Report
  • [Remarks] 長崎大学医学部薬理

    • URL

      http://www.med.nagasaki-u.ac.jp/phrmch1/publication/publication.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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