Dynamic regulation of cell differentiation via gene regulatory networks organized by multiple transcription factors

• Project/Area Number: 24790271
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: OCHIAI Kyoko 東北大学, 医学(系)研究科(研究院), 助教 (10455785)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 遺伝子発現制御

Research Abstract

Cell type specific transcription factor (TF) regulate cell differentiation via the organization of gene regulatory network (GRN). The aim of this research is focused on the chain reaction of GRNs caused by two different TFs, which have the relation of up- and downstream. The GRN organized by FoxO1, the upstream TF, have been clarified in our previous study. The GRNs organized by the downstream TF, which are the direct target of FoxO1 and activated via FoxO1-GRN, have analyzed. Our new GRNs model will present the molecular mechanism for promoting cell differentiation via multiple TFs.

Research Products

• [Journal Article] Orchestrating B lymphopoiesis through interplay of IL-7R and pre-BCR signaling (2014)
  • Author(s): Marcus R. Clark, Malay Mandal, Kyoko Ochiai, Harinder Singh
  • Journal Title: Nature Reviews Immunology Volume: 14(2) Pages: 69-80
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of germinal center B and plasma cell fates by dynamical control of IRF4 (2013)
  • Author(s): Kyoko Ochiai, Mark Maienschein-Cline, Jianjun Chen, Anita S. Chong, Aaron R. Dinner, Harinder Singh, Roger Sciammas
  • Journal Title: Immunity Volume: 38(5) Pages: 918-29
  • Peer Reviewed
• [Journal Article] A self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation (2012)
  • Author(s): Kyoko Ochiai, Maienschein-Cline, Malay Mandal, Joseph R Triggs, Eric Bertolino, Roger Sciammas, Aaron R Dinner, Marcus R Clark, Harinder Singh
  • Journal Title: Nature Immunology Volume: 13(3) Pages: 300-307
  • Peer Reviewed
• [Presentation] Organization of Plasma Cell Differentiation via Epigenome-related Complex (2013)
  • Author(s): 落合恭子, 五十嵐和彦
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-06
• [Presentation] Regulation of Mat2α-mediated cell differentiation via nuclear organization (2013)
  • Author(s): Kyoko Ochiai, Hiroki Shima, Kazuhiko Igarashi
  • Organizer: Immunity & Tolerance, the LXXVIII Cold Spring Harbor Symposium on Quantitative Biology
  • Place of Presentation: New York, USA