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Molecular analysis of immune privilege in pluripotent stem cells

Research Project

Project/Area Number 24790276
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

OKITA Keisuke  京都大学, iPS細胞研究所, 講師 (90512434)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords再生医学 / 多能性幹細胞 / iPS細胞 / 免疫応答 / カニクイザル / 移植 / 初期化
Research Abstract

Induced pluripotent stem (iPS) cells showed slight upregulation of HLA expression by the stimulation with IFNgamma, but it was ten fold lower than that in fibroblasts. On the other hand, we have recently developed an efficient method to make iPS cells from human peripheral blood. This study enabled us to establish iPS cells from Macaca fascicularis. Transplantation of differentiated cells from monkey iPS cells can be a useful model to examine human immune response after transplantation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (25 results)

All 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (12 results) (of which Invited: 10 results) Book (4 results) Remarks (4 results)

  • [Journal Article] Direct Comparison of Autologous and Allogeneic Transplantation of iPSC Derived Neural Cells in the Brain of a Nonhuman Primate Stem2013

    • Author(s)
      Morizane A, Doi D, Kikuchi T, Okita K, Hotta A, Kawasaki T, Hayashi T, Onoe H, Shiina T, Yamanaka S, Takahashi J.
    • Journal Title

      Stem Cell Reports

      Volume: 1 Issue: 4 Pages: 1-10

    • DOI

      10.1016/j.stemcr.2013.08.007

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A drug screening platform for Alzheimer's disease with intracellular Aβ oligomers using patient-specific iPSCs2013

    • Author(s)
      Kondo, T., (他24名), Murakami, K., Irie, K., Klein, W. L., Mori, H., Asada, T., Takahashi, R., Iwata, N., Yamanaka, S., Inoue, H
    • Journal Title

      Cell Stem Cell

      Volume: 12 Issue: 4 Pages: 487-496

    • DOI

      10.1016/j.stem.2013.01.009

    • Related Report
      2012 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells2012

    • Author(s)
      Okita K, Yamakawa T, et al.
    • Journal Title

      Stem Cells

      Volume: Volume 31, Issue 3 Issue: 3 Pages: 458-466

    • DOI

      10.1002/stem.1293

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells.2012

    • Author(s)
      Kajiwara M
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 109 Issue: 31 Pages: 12538-12543

    • DOI

      10.1073/pnas.1209979109

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Drug screening for ALS using patient-specific induced pluripotent stem cells2012

    • Author(s)
      Egawa, N., Kitaoka, S., Tsukita, K., Naitoh, M., Takahashi, K., Yamamoto, T., Adachi, F., Kondo, T., Okita, K., Asaka, I., Aoi, T., Watanabe, A., Yamada, Y., Morizane, A., Takahashi, J., Ayaki, T., Ito, H., Yoshikawa, K., Yamawaki, S., Suzuki, S., Watanabe, D., Hioki, H., Kaneko, T., Makioka, K., Okamoto, K., Takuma, H., Tamaoka, A., Hasegawa, K., Nonaka, T., Hasegawa, M., Kawata, A., Yoshida, M., Nakahata, T., Takahashi, R., Marchetto, M. C. N., Gage, F. H., Yamanaka, S., and Inoue, H
    • Journal Title

      Sci. Transl. Med.

      Volume: 4 Issue: 145 Pages: 1-8

    • DOI

      10.1126/scitranslmed.3004052

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] iPS細胞の臨床応用を目指して日本薬学会関東支部2013

    • Author(s)
      沖田圭介
    • Organizer
      第38回学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2013-12-22
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞研究の最前線から岩形態が変形しディッシュから剥離していき、安定した培養と実験系の構築は困難であった2013

    • Author(s)
      沖田圭介
    • Organizer
      国市科学センター市民科学講座
    • Place of Presentation
      山口
    • Year and Date
      2013-03-16
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞を、医療へ2013

    • Author(s)
      沖田圭介
    • Organizer
      大阪大学付属病院未来医療センター10周年・未来医療開発部開設記念シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2013-01-13
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] iPS細胞の臨床応用を目指して2013

    • Author(s)
      沖田 圭介
    • Organizer
      日本薬学会関東支部 第38回学術講演会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS細胞を、医療へ2013

    • Author(s)
      沖田圭介
    • Organizer
      大阪大学付属病院 未来医療センター10周年・未来医療開発部開設記念シンポジウム
    • Place of Presentation
      大阪、日本
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] iPS細胞研究の最前線から2013

    • Author(s)
      沖田圭介
    • Organizer
      岩国市科学センター 市民科学講座
    • Place of Presentation
      山口、日本
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] iPS細胞の臨床応用を目指して2012

    • Author(s)
      沖田圭介
    • Organizer
      北海道大学第一回IGM特別セミナー
    • Place of Presentation
      北海道
    • Year and Date
      2012-12-20
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Re-characterization of iPS cells2012

    • Author(s)
      Keisuke Okita
    • Organizer
      Netherlands - Japan Cross Debate Workshop on RM and Stem cells
    • Place of Presentation
      神奈川
    • Year and Date
      2012-06-17
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Immunogenic potential of mouse pluripotent stem cells2012

    • Author(s)
      Keisuke Okita, Yasuko Matsumura, Yoshiko Sato, and Shinya Yamanaka
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      神奈川
    • Year and Date
      2012-06-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Immunogenic potential of mouse pluripotent stem cells2012

    • Author(s)
      Keisuke Okita, Yasuko Matsumura, Yoshiko Sato, and Shinya Yamanaka
    • Organizer
      ISSCR 10th annual Meeting
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Re-characterization of iPS cells2012

    • Author(s)
      Keisuke Okita
    • Organizer
      Netherlands - Japan Cross Debate Workshop on RM and Stem cells
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] iPS細胞の臨床応用を目指して2012

    • Author(s)
      沖田圭介
    • Organizer
      北海道大学第一回IGM特別セミナー
    • Place of Presentation
      札幌、北海道
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 細胞工学2012

    • Author(s)
      島本廉, 沖田圭介
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2013 Final Research Report
  • [Book] 実験医学増刊2012

    • Author(s)
      山川達也, 沖田圭介
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Book] 細胞工学2012

    • Author(s)
      島本廉、沖田圭介
    • Total Pages
      6
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2012 Research-status Report
  • [Book] 実験医学増刊2012

    • Author(s)
      山川達也,沖田圭介
    • Total Pages
      6
    • Publisher
      羊土社
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.cira.kyoto-u.ac.jp/okita/

    • Related Report
      2013 Final Research Report
  • [Remarks] 京都大学iPS細胞研究所

    • URL

      http://www.cira.kyoto-u.ac.jp/j/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 京都大学iPS細胞研究所沖田グループ

    • URL

      http://www.cira.kyoto-u.ac.jp/okita/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.cira.kyoto-u.ac.jp/okita/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2025-11-19  

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