Molecular analysis of TMEM16 family members

• Project/Area Number: 24790278
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: SUZUKI Jun 京都大学, 医学(系)研究科(研究院), 助教 (30511894)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: TMEM16F / TMEM16 / Xkr8 / Xkr / Scramblase / リン脂質スクランブリング / ホスファチジルセリン / アポトーシス / リン脂質 / スクランブル活性 / クロライドチャネル / phosphatidylserine / phophatidylcholine / phosphatidylcholine / galactosylceramide

Outline of Final Research Achievements

Phospholipids are asymmetrically distributed in plasma membrans. However, its distribution is disrupted by phospholipid scramblases, which transport lipids bidirectionally and nonspecifically. In this study, I investigated molecular functions of TMEM16 family members, and found that TMEM16C, D, F, G, and J possessed phospholipid scrambling activity.

Research Products

• [Journal Article] Exposure of phosphatidylserine by Xk-related protein family members during apoptosis (2014)
  • Author(s): Suzuki, J., Imanishi, E. and Nagata, S.
  • Journal Title: J. Biol. Chem Volume: 289 Issue: 44 Pages: 30257-30267
  • DOI: 10.1074/jbc.m114.583419
  • Peer Reviewed
• [Journal Article] Flippases and scramblases in the plasma membrane (2014)
  • Author(s): Segawa, K., Suzuki, J. and Nagata, S.
  • Journal Title: Cell Cycle Volume: 13 Issue: 19 Pages: 2990-2991
  • DOI: 10.4161/15384101.2014.962865
  • Peer Reviewed
• [Journal Article] Phospholipid Flippase Activities and Substrate Specificities of Human Type IV P-type ATPases Localized to the Plasma Membrane (2014)
  • Author(s): Takatsu, H., Tanaka, G., Segawa, K., Suzuki, J., Nagata, S., Nakayama, K. and Shin, H.W.
  • Journal Title: J. Biol. Chem Volume: 289 Issue: 48 Pages: 33543-33556
  • DOI: 10.1074/jbc.m114.593012
  • NAID: 120005690224
  • Peer Reviewed / Open Access
• [Journal Article] Functional Swapping between Transmembrane Proteins TMEM16A and TMEM16F (2014)
  • Author(s): Suzuki, T., Suzuki, J. and Nagata, S.
  • Journal Title: J Biol Chem Volume: 289 Issue: 11 Pages: 7438-7447
  • DOI: 10.1074/jbc.m113.542324
  • Peer Reviewed
• [Journal Article] Phospholipid scrambling on the plasma membrane (2014)
  • Author(s): Jun Suzuki, Shigekazu Nagata
  • Journal Title: Methods in Enzymology
  • NAID: 120005458296
  • Peer Reviewed
• [Journal Article] アポトーシス細胞のホスファチジルセリンの露出に関与するXkr8の同定 (2014)
  • Author(s): 鈴木淳、長田重一
  • Journal Title: 実験医学 Volume: 32 Pages: 74-77
• [Journal Article] リン脂質スクランブラーゼ (2014)
  • Author(s): 鈴木淳
  • Journal Title: 医学のあゆみ Volume: 248 Pages: 1137-1142
• [Journal Article] Xk-related protein 8 and CED-8 promote phosphatidylserine exposure in apoptotic cells. (2013)
  • Author(s): Suzuki, J., Denning, DP., Imanishi, E., Horvitz, HR. and Nagata, S.
  • Journal Title: Science Volume: 341 Issue: 6144 Pages: 403-406
  • DOI: 10.1126/science.1236758
  • NAID: 120005300357
  • Peer Reviewed
• [Journal Article] Calcium-dependent Phospholipid Scramblase Activity of TMEM16 Family Members. (2013)
  • Author(s): Suzuki J, Fujii T, Imao T, Ishihara K, Kuba H, Nagata S.
  • Journal Title: J Biol Chem Volume: なし
  • Peer Reviewed
• [Presentation] Identification of phospholipid-scrambling proteins (2015)
  • Author(s): Jun Suzuki, Shigekazu Nagata
  • Organizer: 6th International Conference on Phospholipases A2 and Lipid Mediators
  • Place of Presentation: Tokyo
  • Year and Date: 2015-02-12
  • Invited
• [Presentation] Identification of phosphatidylserine-exposing protein (2014)
  • Author(s): Jun Suzuki
  • Organizer: International Meeting of the Advanced Cancer Biology in memory of Dr. Hidesaburo Hanafusa
  • Place of Presentation: Nagoya
  • Year and Date: 2014-10-26
  • Invited
• [Presentation] 細胞膜リン脂質のスクランブル機構 (2014)
  • Author(s): 鈴木 淳、長田 重一
  • Organizer: 日本生化学会大会 シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2014-10-16
  • Invited
• [Presentation] 細胞膜リン脂質スクランブルの分子機構の解明 (2014)
  • Author(s): 鈴木 淳
  • Organizer: 日本生化学会 奨励賞受賞講演
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15
  • Invited
• [Presentation] Molecular Mechanism of phosphatidylserine exposure (2014)
  • Author(s): Jun Suzuki
  • Organizer: RIKEN BSI FORUM
  • Place of Presentation: Saitama
  • Year and Date: 2014-09-01
  • Invited
• [Presentation] リン脂質のスクランブル機構 (2014)
  • Author(s): 鈴木 淳
  • Organizer: 名古屋大学生命理学科セミナー
  • Place of Presentation: 名古屋
  • Year and Date: 2014-07-01
  • Invited
• [Presentation] Exposure of phosphatidylserine by Xkr8 (2013)
  • Author(s): Jun Suzuki, Shigekazu Nagata
  • Organizer: The CSHL Cell Death Meeting
  • Place of Presentation: Cold Spring Harbor Laboratory
• [Presentation] Phospholipid scrambling on plasma membrane (2012)
  • Author(s): Jun Suzuki, Shigekazu Nagata
  • Organizer: Kyoto University Global COE "Center for Frontier Medicine"Retreat 2012
  • Place of Presentation: Awajishima
  • Invited
• [Presentation] 細胞膜リン脂質のスクランブル機構
  • Author(s): 鈴木淳、長田重一
  • Organizer: 生命科学阿波踊りシンポジウム
  • Place of Presentation: 徳島大学
  • Invited
• [Presentation] 細胞膜リン脂質のスクランブル機構
  • Author(s): 鈴木淳、長田重一
  • Organizer: 日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] 細胞膜リン脂質のスクランブル機構
  • Author(s): 鈴木淳
  • Organizer: 奈良先端未来開拓コロキウム
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Invited
• [Presentation] Mechanism of Phosphatidylserine Exposure in Apoptotic Cells
  • Author(s): 鈴木淳、長田重一
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 細胞膜リン脂質のスクランブル機構
  • Author(s): 鈴木 淳、長田 重一
  • Organizer: 日本膜学会第34年会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 細胞膜リン脂質のスクランブル機構
  • Author(s): 鈴木 淳、長田 重一
  • Organizer: 第7回トランスポーター研究会年会
  • Place of Presentation: 京都
  • Invited
• [Remarks] アポトーシス時のリン脂質暴露に関与する因子の同定
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2013/130712_1.htm