Selective secretion mechanism of Wnt in polarized epithelial cells

• Project/Area Number: 24790280
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Dokkyo Medical University
• Principal Investigator: SAKANE Hiroshi 獨協医科大学, 医学部, 助教 (80457291)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞内シグナル伝達 / Wnt / リサイクリング / 上皮細胞 / 選別輸送 / クラスリン

Research Abstract

Selective basolateral secretion of Wnt3a in polarized epithelial cells was regulated by Wntless, clathrin and clathrin adaptor proteins. We found that Wntless was localized to the basolateral membrane. We also found that Wnt11 was sorted to the apical side and glycosylation was involved in the selective secretion of Wnt proteins. Furthermore, we found that a-taxilin, a syntaxin4 binding protein, was involved in the recycling of transferrin receptor. These results clarified a part of mechanism of selective secretion of Wnt in polarized epithelial cells and the function of regulator of intracellular vesicle traffic.

Research Products

• [Journal Article] α-Taxilin interacts with soring nexin 4 and participates in the recycling pathway of transferrin receptor. (2014)
  • Author(s): Sakane, H., Horii, Y., Nogami, S., Kawano, Y., Kaneko-Kawano, T., and Shirataki, H.
  • Journal Title: PloS ONE Volume: 9 Issue: 4 Pages: e93509-e93509
  • DOI: 10.1371/journal.pone.0093509
  • Peer Reviewed / Open Access
• [Journal Article] The apical and basolateral secretion of Wnt11 and Wnt3a in polarized epithelial cells is regulated by distinct mechanisms. (2013)
  • Author(s): Yamamoto, H., Awada, C., Hanaki, H., Sakane, H., Tsujimoto, I., Takahashi, Y., Takao, T., and Kikuchi, A.
  • Journal Title: J. Cell Sci. Volume: 126 Pages: 2931-2943
  • DOI: 10.1242/jcs.126052
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/molbiobc/
• [Remarks]
  • URL: http://www.dokkyomed.ac.jp/dep-m/mol-cell-bio/index.html