The role of phosphoinositide metabolism in the interaction of different cell types in skin and tumor

• Project/Area Number: 24790295
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: NAKAMURA Yoshikazu 東京薬科大学, 生命科学部, 講師 (60366416)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ホスホリパーゼC / 皮膚

Research Abstract

The interaction of different cell types is important in the maintenance of skin functions. We found that epidermal loss of phospholipase c (PLC) delta1 upregulated interleukin-23 and generated partially similar environment to tumor microenvironment. We also found that subcutaneous injection of B16-F10 cells into keratinocyte-specific PLC delta1 knockout mice enhanced the growth and metastasis of B16-F10 cells.

Research Products

• [Journal Article] Simultaneous loss of phospholipase Cδ1 and phospholipase Cδ3 causes cardiomyocyte apoptosis and cardiomyopathy (2014)
  • Author(s): Nakamura, Y., Kanemaru, K., Kojima, R., Hashimoto, Y., Marunouchi, T., Oka, N., Ogura, T., Tanonaka, K., and Fukami, K
  • Journal Title: Cell Death Dis Volume: 5
• [Journal Article] The role of phospholipase C isozymes in cellular homeostasis (2014)
  • Author(s): Fukami, K., and Nakamura, Y
  • Journal Title: Phospholipases in Health and Disease Volume: 10 Pages: 201-210
• [Journal Article] Simultaneous Loss of Phospholipase Cδ1 and Phospholipase Cδ3 Causes Cardiomyocyte Apoptosis and Cardiomyopathy. (2014)
  • Author(s): Y Nakamura, K Kanemaru, et al.
  • Journal Title: Cell Death & Disease Volume: 5 Issue: 5 Pages: 140-147
  • DOI: 10.1038/cddis.2014.181
  • Peer Reviewed / Open Access
• [Journal Article] Genetic ablation of phospholipase C delta 1 increases survival in SOD1(G93A) mice (2013)
  • Author(s): Staats, KA., Van, H. L., Jones, A. R., Bento, A. A., Van, H. A., Shatunov, A., Simpson. C.L., Lemmens. R., Jaspers. T., Fukami, K., Nakamura, Y., Brown, RH. Jr., Van, D. P., Liston, A., Robberecht, W., Al-Chalabi. A., and Van, D. B.L
  • Journal Title: Neurobiol. Dis Volume: 60 Pages: 11-7
• [Journal Article] Physiological functions of phospholipase Cδ1 and phospholipase (2013)
  • Author(s): Nakamura. Y., Kanemaru, K., and Fukami, K
  • Journal Title: Cδ3. Adv. Biol. Regul Volume: 53 Pages: 356-62
• [Journal Article] Genetic ablation of phospholipase C delta 1 increases survival in SOD1(G93A) mice. (2013)
  • Author(s): 1. Staats, KA., Van, H. L., Jones, A. R., Bento, A. A., Van, H. A., Shatunov, A., Simpson. C.L., Lemmens. R., Jaspers. T., Fukami, K., Nakamura, Y., Brown, RH. Jr, Van, D. P., Liston, A., Robberecht, W., Al-Chalabi. A., and Van, D. B.L.
  • Journal Title: Neurobiol. Dis. Volume: 60 Pages: 11-17
  • DOI: 10.1016/j.nbd.2013.08.006
  • Peer Reviewed
• [Journal Article] Physiological functions of phospholipase Cδ1 and phospholipase Cδ3. (2013)
  • Author(s): Y Nakamura, K Kanemaru, K Fukami.
  • Journal Title: Adv Biol Regul. Volume: 53 Issue: 3 Pages: 356-362
  • DOI: 10.1016/j.jbior.2013.07.003
  • Peer Reviewed
• [Journal Article] Epidermal phospholipase Cδ1 regulates granulocyte counts and systemic interleukin 17 levels in mice (2012)
  • Author(s): Kanemaru, K., Nakamura Y., Sato, K., Kojima, R., Takahashi, S., Yamaguchi, M., Ichinohe, M., Kiyonari, H., Shioi, G., Kabashima, K., Nakahigashi, K., Asargiri, M., Jamora, C., Yamaguchi, H., and Fukami, K
  • Journal Title: Nat. Commun (1st authors and corresponding authors) Volume: 963
• [Journal Article] Epidermal phospholipase C δ1 regulates granulocyte counts and systemic interleukin-17 levels in mice. (2012)
  • Author(s): Kaori Kanemaru
  • Journal Title: Nat. Commun. Volume: 3 Issue: 1 Pages: 963-963
  • DOI: 10.1038/ncomms1960
  • Peer Reviewed
• [Presentation] Phospholipase Cδ1 Regulates Keratinocyte Proliferation and differentiation (2013)
  • Author(s): 1. Ogura, T., Kanemaru, K., Nakamura, Y., and Fukami, K
  • Organizer: The 8th Japan - Korea Conference on Cellular Signaling for Young Scientists
  • Place of Presentation: Fukuoka
• [Presentation] 酸性リン脂質代謝酵素ホスホリパーゼC (PLC)δ1とδ3の生理機能解析 (2013)
  • Author(s): 2.深見希代子、金丸佳織、中村由和
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 細胞膜リン脂質代謝酵素ホスホリパーゼCδ1によるケラチノサイトの増殖 (2013)
  • Author(s): 3.中村由和、金丸佳織、深見希代
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Epidermal phospholipase Cδ1 regulates systemic interleukin 17 levels and granulocyte counts in mice (2013)
  • Author(s): 4. Kanemaru, K., Nakamura, Y., and Fukami, K
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] ホスホリパーゼCδ1はケラチノサイトの増殖・分化を制御する (2013)
  • Author(s): 5.岡臨、小倉崇寛、金丸佳織、中村由和、深見希代子
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] イノシトールリン脂質代謝酵素と皮膚疾患の関連についての検討 (2013)
  • Author(s): 6.新屋有希、金丸佳織、中村由和、深見希代子
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 肥満合併による乾癬悪化を再現するマウスモデルの確立 (2013)
  • Author(s): 7.松雪綾乃、金丸佳織、中村由和、深見希代子
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] ホスホリパーゼCδ1はケラチノサイトの増殖・分化を制御する (2013)
  • Author(s): 8.小倉崇寛、金丸佳織、中村由和、深見希代子
  • Organizer: 第55回脂質生化学会
  • Place of Presentation: 松島
• [Presentation] ホスホリパーゼCδ1の欠損は炎症性皮膚疾患を誘導する (2013)
  • Author(s): 9.金丸佳織、中村由和、深見希代子
  • Organizer: 第55回脂質生化学会
  • Place of Presentation: 松島
• [Presentation] Loss of phospholipase Cδ1 impairs epidermal barrier and induces chronic dermatitis (2013)
  • Author(s): Kanemaru, K., Nakamura, Y., and Fukami, K
  • Organizer: International Investigative Dermatology
  • Place of Presentation: Edinburgh
• [Presentation] Keratinocyte-specific loss of Phospholipase Cδ1 results in aberrant activation of IL-23/IL-17 axis and skin inflammation (2013)
  • Author(s): Nakamura, Y., Kanemaru, K., and Fukami, K
  • Organizer: International Investigative Dermatology
  • Place of Presentation: Edinburgh
• [Presentation] Keratinocyte-specific loss of Phospholipase Cδ1 results in aberrant activation of IL-23/IL-17 axis and skin inflammation. (2013)
  • Author(s): Nakamura, Y., Kanemaru, K., and Fukami, K.
  • Organizer: International Investigative Dermatology
  • Place of Presentation: Edinburgh
• [Presentation] Phospholipase Cδ1 Regulates Keratinocyte Proliferation and differentiation (2013)
  • Author(s): Ogura, T., Kanemaru, K., Nakamura, Y., and Fukami, K.
  • Organizer: The 8th Japan-Korea Conference on Cellular Signaling for Young Scientists
  • Place of Presentation: 福岡
• [Presentation] 酸性リン脂質代謝酵素ホスホリパーゼC (PLC)δ1とδ3の生理機能解析 (2013)
  • Author(s): 深見希代子、金丸佳織、中村由和
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 細胞膜リン脂質代謝酵素ホスホリパーゼCδ1によるケラチノサイトの増殖、分化制御と表皮バリア形成 (2013)
  • Author(s): 中村由和、金丸佳織、深見希代子
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Epidermal phospholipase Cδ1 regulates systemic interleukin-17 levels and granulocyte counts in mice (2013)
  • Author(s): Kanemaru, K., Nakamura, Y., and Fukami, K.
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] ホスホリパーゼCδ1はケラチノサイトの増殖・分化を制御する (2013)
  • Author(s): 岡臨、小倉崇寛、金丸佳織、中村由和、深見希代子
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] イノシトールリン脂質代謝酵素と皮膚疾患の関連についての検討 (2013)
  • Author(s): 新屋有希、金丸佳織、中村由和、深見希代子
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 肥満合併による乾癬悪化を再現するマウスモデルの確立 (2013)
  • Author(s): 松雪綾乃、金丸佳織、中村由和、深見希代子
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Phospholipase Cδ1 is required for normal expression of profilaggrin in keratinocytes (2012)
  • Author(s): Nakamura, Y., Kanemaru, K., Kabashima, K., Nakahigashi, K., and Fukami, K
  • Organizer: 第37回日本研究皮膚科学会
  • Place of Presentation: 沖縄
• [Presentation] Epidermal phospholipase Cδ1 regulates systemic interleukin 17 levels and granulocyte counts in mice (2012)
  • Author(s): Kanemaru, K., Nakamura Y., Kabashima, K., Nakahigashi, K., and Fukami, K
  • Organizer: 第37回日本研究皮膚科学会
  • Place of Presentation: 沖縄
• [Presentation] Phospholipase Cδ1は表皮角化細胞におけるプロフィラグリンの正常な発現に必須である (2012)
  • Author(s): 14.中村由和、金丸佳織、深見希代子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 表皮におけるホスホリパーゼCδ1の欠損は血清中のIL-17濃度を上昇させ顆粒球増多を引き起こす (2012)
  • Author(s): 15.金丸佳織、中村由和、深見希代子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] ホスホリパーゼCδ1欠損マウスはペントバルビタール麻酔に対する感受性が低下する (2012)
  • Author(s): 16.山口眞美、金丸佳織、中村由和、深見希代子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Loss of epidermal phospholipase Cδ1 results in IL-23/IL-17 axis activation and granulocytosis (2012)
  • Author(s): 17. Kanemaru, K., Nakamura, Y., and Fukami, K
  • Organizer: 42nd Annual European Society for Dermatological Research Meeting
  • Place of Presentation: Venice, ITALY
• [Presentation] ホスホリパーゼCδ1とδ3は正常な心機能の維持に重要である (2012)
  • Author(s): 18.橋本悠希、児島亮太、中村由和、深見希代子
  • Organizer: 第54回日本脂質生化学会
  • Place of Presentation: 福岡
• [Presentation] 表皮特異的なPLCδ1の欠損は乾癬様皮膚炎を引き起こす (2012)
  • Author(s): 19.金丸佳織、中村由和、椛島健治、深見希代子
  • Organizer: 第54回日本脂質生化学会
  • Place of Presentation: 福岡
• [Presentation] ホスホリパーゼCδ1はアトピー性皮膚炎発症因子フィラグリンの発現を制御する (2012)
  • Author(s): 20.中村由和、金丸佳織、椛島健治、深見希代子
  • Organizer: 第54回日本脂質生化学会
  • Place of Presentation: 福岡
• [Presentation] ホスホリパーゼCδ1はアトピー性皮膚炎発症因子フィラグリンの発現を制御する (2012)
  • Author(s): 中村由和 他
  • Organizer: 第５４回日本脂質生化学会
  • Place of Presentation: 九州大学（福岡）
• [Presentation] Phospholipase C δ1 is required for normal expression of profilaggrin in keratinocytes (2012)
  • Author(s): 中村由和 他
  • Organizer: 第37回日本研究皮膚科学会
  • Place of Presentation: ロジワールホテル（沖縄）
• [Presentation] Phospholipase Cδ1は表皮角化細胞におけるプロフィラグリンの正常な発現に必須である (2012)
  • Author(s): 中村由和 他
  • Organizer: 第８５回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡(福岡)
• [Remarks]
  • URL: http://logos.ls.toyaku.ac.jp/~genome/Site/pages/main.html