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Roles of glycosphingolipids in enterocytes for lipid absorption

Research Project

Project/Area Number 24790298
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionChubu University

Principal Investigator

TAJIMA Orie  中部大学, 生命健康科学部, 准教授 (10362237)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsガングリオシド / 脂肪酸吸収 / 脂質ラフト / 脂肪酸輸送体 / スフィンゴ糖脂質 / 腸管上皮細胞
Outline of Final Research Achievements

Double knockout (DKO) mice of GM2/GD2 synthase and GD3 synthase lacked all ganglio-series gangliosides including asialo-series, except GM3, despite the fact that composition of glycosphingolipids (GSLs) in small intestine of wild type (WT) mice was converted from GM3, GM1 and GD1a to GA1 during sucking-to-weanling transition. Analysis of fractionated membrane extracts revealed that fundamental molecules including cholesterol and galectin-4 dispersed from the GEM/rafts in the DKO mice. As for floating patterns of lipid-binding proteins, levels of raft-associated FAT/CD36 and FATP4 were significantly decreased in the DKO mice, while these molecules appeared in both raft and non-raft fractions in the wild type mice. Isolated enterocytes from the DKO mice showed significantly reduced fatty acid uptake. These results strongly suggested that proper composition of GSL is critical to regulate architecture and functions of GEM/rafts, and fatty acid uptake via these microdomains.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (8 results)

  • [Journal Article] Ganglioside deficiellcy causes inflammation and neurodegeneration via the activation of complement system in the spinal cord.2014

    • Author(s)
      Yuhsuke Ohmi, Yuki Ohkawa, et al.
    • Journal Title

      Journal of Neuroinflammation

      Volume: 61(印刷中) Issue: 1 Pages: 61-61

    • DOI

      10.1186/1742-2094-11-61

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] UVB-irradiated keratinocytes induce melanoma-associated ganglioside GD3 synthase gene in melanocytes via secretion of tumor necrosis factor α and interleukin 6.2014

    • Author(s)
      Miyata M.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 445 Issue: 2 Pages: 504-10

    • DOI

      10.1016/j.bbrc.2014.02.038

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Essential roles of gangliosides in the formation and maintenance of membrane microdomains in brain tissues.2012

    • Author(s)
      Ohmi Y, Ohkawa Y, Yamauchi Y, Tajima O, Furukawa K, Furukawa K
    • Journal Title

      Neurochem Res

      Volume: 36(9) Pages: 578-86

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 糖脂質糖鎖改変マウスにおける脂質代謝障害の分子メカニズム2014

    • Author(s)
      田島織絵、桐谷美咲、古川鋼一、古川圭子
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都府・京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糖鎖変異に伴う脂質代謝障害の検討2014

    • Author(s)
      田島織絵、桐谷美咲、古川鋼一、古川圭子
    • Organizer
      第33回日本糖質学会大会
    • Place of Presentation
      愛知県・名古屋
    • Year and Date
      2014-08-10 – 2014-08-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糖鎖変異による脂質吸収障害の分子メカニズム2013

    • Author(s)
      田島織絵、藤田雄輝、桐谷美咲、雨宮慶、宮田麻衣子、徳田典代、田口良、古川鋼一、古川圭子
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] アストロサイトにおけるガングリオシド糖鎖の機能2013

    • Author(s)
      大海 雄介、大川 祐樹、姫 シュウテイ、大平 拡、田島 織絵、古川 圭子、古川 鋼一
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] TNFα及び紫外線によるメラノサイトとメラノーマのGD3合成酵素遺伝子の発現誘導2013

    • Author(s)
      古川 圭子、宮田 麻衣子、田島 織絵、神戸 真理子、祖父江 沙矢加、市原 正智、杉浦 一充、古川 鋼一
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 糖鎖変異による小腸機能障害の分子メカニズム2012

    • Author(s)
      田島織絵、藤田雄輝、宮田麻衣子、徳田典代、田口良、大海雄介、大川祐樹、古川圭子、古川鋼一
    • Organizer
      第32回日本糖質学会大会
    • Place of Presentation
      鹿児島、日本
    • Related Report
      2012 Research-status Report
  • [Presentation] 腸管上皮細胞におけるスフィンゴ糖脂質の役割2012

    • Author(s)
      田島織絵、藤田雄輝、宮田麻衣子、徳田典代、田口良、大海雄介、大川祐樹、古川鋼一、古川圭子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡、日本
    • Related Report
      2012 Research-status Report
  • [Presentation] GM3-onlyマウスの中枢神経系で高発現するPerilipin4はミクログリアに発現しリソソーム機能を亢進させる2012

    • Author(s)
      大川祐樹、大海雄介、田島織絵、百田洋之、加藤彰、夏目敦至、若林俊彦、古川圭子、古川鋼一
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡、日本
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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