Activity-dependent calcium signaling plays a role in development and maturation of cerebellar granule cells

• Project/Area Number: 24790300
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: National Cardiovascular Center Research Institute (2013-2014); Osaka Bioscience Institute (2012)
• Principal Investigator: OKAZAWA Makoto 独立行政法人国立循環器病研究センター, 研究所, 室長 (40414130)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 転写因子 / 神経成熟 / 膜電位 / 小脳顆粒細胞 / 静止膜電位 / 遺伝子発現 / カルシウムシグナル / シグナル伝達

Outline of Final Research Achievements

In many developing neuronal cell types, the resting membrane potential shift from a depolarized state to a non-depolarized state during the early postnatal period. The shift of the resting membrane potential and the resulting inactivation of calcineurin are essential for activity-dependent maturation gene expression via Etv1 transcription factor and for morphological and electrophysiological maturation in cerebellar granule cells. In this study we analyzed the signaling mechanism of maturation gene expression and of immature gene suppression during cerebellar network formation. We found that Etv1 was regulated not only by activity-dependent calcium signaling but also by TrkB-ERK signaling and that Etv1 was associated with a transcriptional corepressor which might regulate immature gene expression.

Research Products

• [Journal Article] Gene regulation via excitation and BDNF is mediated by induction and phosphorylation of the Etv1 transcription factor in cerebellar granule cells (2012)
  • Author(s): Abe, H., Okazawa, M. & Nakanishi, S.
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 109 Issue: 22 Pages: 8734-8739
  • DOI: 10.1073/pnas.1206418109
  • Peer Reviewed
• [Presentation] LOX-1 遺伝子欠損SHRSPの作製とその形質の予備的解析 (2014)
  • Author(s): 垣野明美、李 蕾、池末昌弘、中野厚史、藤田佳子、岡澤 慎、真下知士、沢村達也
  • Organizer: 第50回高血圧関連疾患モデル学会学術総会
  • Place of Presentation: 和歌山
  • Year and Date: 2014-12-05 – 2014-12-06
• [Presentation] cGMP依存性蛋白キナーゼによるTASK電流の調節 (2014)
  • Author(s): 齋藤充、田中千恵、古谷和春、岡澤慎、豊田博紀、佐藤元、倉智嘉久、姜英男
  • Organizer: 第37会日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11 – 2014-09-13