| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
IMSMP-1 cells derived from lung tumor spontaneously developed in cadm1-deficient mice were constitutively expressed with GFP, and injected into spleen of nude mice. In the following day, tumor cells invaded into liver can be seen by intravital imaging using multi-photon laser microscope. Then, we injected adult T-cell leukemia (ATL)-related MT-2 cells into the tail vein of NOG mice and established the mouse model of human ATL with multi-organ infiltration. MT-2 cells were then constitutively transfected with expression vectors of shCADM1 and GFP. One day after injection, the extravasation of MT-2 cells into liver were reduced in shCADM1-expressing cells compared with shControl-expressing cells, indicating that the expression of CADM1 is important for the early stage of ATL cell invasion.
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