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The role of HSF4 in proteostasis

Research Project

Project/Area Number 24790316
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pathological medical chemistry
Research InstitutionYamaguchi University

Principal Investigator

TAKAKI Eiichi  山口大学, 医学(系)研究科(研究院), 学術研究員 (50525590)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords熱ショック応答 / 転写因子 / 熱ショック因子 / 転写制御 / ストレス応答
Research Abstract

Heat shock factors (HSFs) maintain protein homeostasis through regulating expression of heat shock proteins (HSPs), and non-HSP proteins in both control and stressed conditions. HSF family members consist of four members in mammals. Among them, HSF4 is required for the maintenance of lens, and HSF4 mutation is associated with human inherited cataract. Recently, it reveals that HSF4 plays an important role in adaption to stresses in other tissues or cell types. Uniquely, HSF4 constitutively forms a trimer, which can bind to DNA. However, it is unclear how the transcriptional activity of HSF4 is regulated in response to environmental or physiological signals. To find factors that regulate HSF4 activity, we performed immunoprecipitation analysis, followed by mass spectrometry. Furthermore, these factors were functionally screened. We identified a novel protein that promotes phosphorylation of HSF4. We propose a possible mechanism of HSF4 regulation by this novel factor.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (4 results)

  • [Journal Article] Genome - wide DNA methylation profiling in cultured eutopic and ectopic endometrial stromal cells2014

    • Author(s)
      ①Yamagata Y, Nishino K, Takaki E, Sato S, Maekawa R, Nakai A, Sugino N
    • Journal Title

      PLoS One

      Volume: 9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide DNA methylation profiling in cultured eutopic and ectopic endometrial stromal cells.2014

    • Author(s)
      Yamagata Y; Nishino K; Takaki E; Sato S; Maekawa R; Nakai A; Sugino N.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 1 Pages: e83612-e83612

    • DOI

      10.1371/journal.pone.0083612

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chicken IL-6 is a heat - shock gene2013

    • Author(s)
      ②Prakasam R., Fujimoto M., Takii R., Hayashida N., Takaki E., Tan K., Wu F., Inouye S. & Nakai A
    • Journal Title

      FEBS Lett

      Volume: 587 Pages: 3541-3547

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome - wide DNA methylation analysis reveals a potential mechanism for the pathogenesis and development of uterine leiomyomas2013

    • Author(s)
      ③Maekawa R., Sato S., Yamagata Y., Asada H., Tamura I., Lee L., Okada M., Tamura H., Takaki E., Nakai A. & Sugino N
    • Journal Title

      PLoS One

      Volume: 8

    • NAID

      120005652007

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chicken IL-6 is a heat-shock gene2013

    • Author(s)
      Prakasam R, Fujimoto M. Takii R, Hayashida N, Takaki E, Tan K, Wu F, Inouye S, Nakai A.
    • Journal Title

      FEBS Lett

      Volume: 587(21) Issue: 21 Pages: 3541-3547

    • DOI

      10.1016/j.febslet.2013.09.012

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide DNA methylation analysis reveals a potential mechanism for the pathogenesis and development of uterine leiomyomas.2013

    • Author(s)
      Maekawa R, Sato S, Yamagata Y, Asada H, Tamura I, Lee L, Okada M, Tamura H, Takaki E, Nakai A, Sugino N
    • Journal Title

      PLoS One

      Volume: 8 Issue: 6 Pages: e66632-e66632

    • DOI

      10.1371/journal.pone.0066632

    • NAID

      120005652007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RPA assists HSF1 access to nucleosomal DNA by recruiting histone chaperone FACT2012

    • Author(s)
      ④Fujimoto M., Takaki E., Takii R., Tan K., Prakasam R., Hayashida N., Iemura S., Natsume T. & Nakai A
    • Journal Title

      Mol. Cell

      Volume: 48 Pages: 182-194

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 熱ショック因子HSF4のターゲット遺伝子の同定2013

    • Author(s)
      高木栄一
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Related Report
      2013 Final Research Report
  • [Presentation] MEF細胞における熱ショック因子HSF4の標的遺伝子の網羅的解析2013

    • Author(s)
      高木栄一
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 熱ショック因子HSF4のリン酸化を増強する因子の同2012

    • Author(s)
      高木栄一
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡(マリンメッセ福岡)
    • Related Report
      2013 Final Research Report
  • [Presentation] 熱ショック因子HSF4のリン酸化を増強する因子の同定2012

    • Author(s)
      高木栄一
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡 (福岡国際会議場・マリンメッセ福岡)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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