Analysis of the role of transcription factors in monocyte development
Project/Area Number |
24790322
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pathological medical chemistry
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Research Institution | Yokohama City University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 単球分化 / 転写因子 / IRF8 / 血球分化 / 単球 / 好中球 / 分化 |
Outline of Final Research Achievements |
We found that the IRF8-KLF4 transcription factor cascade is essential for monocyte development. During IRF8-dependent monocyte differentiation, the chromatin binding of IRF8 at promoter-distal regions induces the establishment of enhancers marked by histone H3 lysine 4 monomethylation (H3K4me1), thereby promoting the expression of monocyte-related genes including KLF4, which is essential for Ly6C+ monocyte development. In IRF8-deficient mice, monocyte development is as defective as that in KLF4-deficient mice. Moreover, mononuclear phagocyte progenitors in IRF8-deficient mice do not express KLF4. We also found that IRF8 suppresses the chromatin binding of C/EBPα, the transcription factor that promotes neutrophil differentiation, to restrain mononuclear phagocyte progenitors from differentiating into neutrophils.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Transcription factor IRF8 plays a critical role in the development of murine basophils and mast cells.2015
Author(s)
H. Sasaki, D. Kurotaki, N. Osato, H. Sato, I. Sasaki, S. Koizumi, H. Wang, C. Kaneda, A. Nishiyama, T. Kaisho, H. Aburatani, H. C. Morse III, K. Ozato, T. Tamura.
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Journal Title
Blood
Volume: 125(2)
Issue: 2
Pages: 358-369
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] IRF8 inhibits C/EBPα activity to restrain mononuclear phagocyte progenitors from differentiating into neutrophils2014
Author(s)
Kurotaki D., Yamamoto M., Nishiyama A., Uno K., Ban T., Ichino M., Sasaki H., Matsunaga S., Yoshinari M., Ryo A., Nakazawa M., Ozato K., Tamura T.
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Journal Title
Nature Communications
Volume: 5
Issue: 1
Pages: 4978-4978
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Integrin α9 on lymphatic endothelial cells regulates lymphocyte egress2014
Author(s)
Ito K, Morimoto J, Kihara A, Matsui Y, Kurotaki D, Kanayama M, Simmons S, Ishii M, Sheppard D, Takaoka A, Uede T
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Journal Title
Proc Natl Acad Sci USA
Volume: 111
Issue: 8
Pages: 3080-3085
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The transcription factor IRF8 counteracts BCR-ABL to rescue dendritic cell development in chronic myelogenous leukemia.2013
Author(s)
Watanabe T, Hotta C, Koizumi S, Miyashita K, Nakabayashi J, Kurotaki D, Sato GR, Yamamoto M, Nakazawa M, Fujita H, Sakai R, Fujisawa S, Nishiyama A, Ikezawa Z, Aihara M, Ishigatsubo Y, Tamura T.
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Journal Title
Cancerresearch
Volume: 73(22)
Issue: 22
Pages: 6642-53
DOI
Related Report
Peer Reviewed
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[Presentation] 転写因子IRF8によるC/EBPαの機能阻害が単核貪食細胞前駆細胞において好中球分化能の喪失をもたらす2015
Author(s)
黒滝大翼, 山本道雄, 西山 晃, 宇野和宏, 藩 龍馬, 市野素英, 佐々木 悠, 松永智子, 吉成正裕, 梁 明秀, 中澤正年, Keiko Ozato, 田村智彦
Organizer
第19回造血器腫瘍研究会
Place of Presentation
グランデはがくれ(佐賀県佐賀市)
Year and Date
2015-01-23
Related Report
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[Presentation] The transcription factor IRF8 is essential for basophil development2014
Author(s)
Sasaki H, Kurotaki D, Osato N, Sato H, Sasaki I, Wang H, Kaneda C, Nishiyama A, Kaisho T, Aburatani H, Morse HC III, Ozato K, Tamura T.
Organizer
第76回日本血液学会学術集会
Place of Presentation
大阪国際会議場(大阪府大阪市)
Year and Date
2014-10-31
Related Report
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[Presentation] 転写因子IRF8によるC/EBPαの機能阻害が単球-樹状細胞前駆細胞における好中球分化能の喪失をもたらす2014
Author(s)
西山 晃, 黒滝大翼, 山本道雄, 宇野和宏, 藩 龍馬, 市野素英, 佐々木悠, 松永智子, 吉成正裕, 梁 明秀, 中澤正年, Keiko Ozato, 田村智彦
Organizer
新学術領域「転写サイクル」班会議
Place of Presentation
慶山(山梨県笛吹市)
Year and Date
2014-08-05
Related Report
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[Presentation] 転写因子IRF8は好塩基球分化に必要である2014
Author(s)
佐々木悠, 黒滝大翼, 大里直樹, 佐藤英明, 佐々木泉, 小泉真一, 金田智香, 西山 晃, 油谷浩幸, 改正恒康, Herbert C. Morse III, Keiko Ozato, 田村智彦
Organizer
新学術領域「転写サイクル」班会議
Place of Presentation
慶山(山梨県笛吹市)
Year and Date
2014-08-05
Related Report
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[Presentation] The IRF8-KLF4 transcription factor cascade critically regulates the development of inflammatory monocytes
Author(s)
Kurotaki D, Osato N, Nishiyama A, Yamamoto M, Ban T, Sato H, Nakabayashi J, Umehara M, Nakazawa M, Miyake N, Matsumoto N, Ozato K, Tamura T
Organizer
The Joint International Meeting of the Japanese Society for Interferon and Cytokine Research and Macrophage Molecular and Cellular Biology 2013
Place of Presentation
都市センターホテル(東京都)
Related Report
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[Presentation] Alpha9 integrin on medullary sinus at draining lymph node regulates lymphocyte egress via controlling S1P secretion during inflammation
Author(s)
Ito K, Matsui Y, Kihara A, Kurotaki D, Kanayama M, Maeda N, Simmons S, Ishii M, Sheppard D, Uede T, Morimoto J
Organizer
100th Annual meeting the American Association of Immunologists
Place of Presentation
Honolulu, The United States of America
Related Report
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[Presentation] The transcription factor IRF8 overrides BCR-ABL to rescue dendritic cell development in chronic myeloid leukemia
Author(s)
Watanabe T, Hotta C, Koizumi S, Miyashita K, Nakabayashi J, Kurotaki D, Sato R G, Yamamoto M, Nishiyama A, Aihara M, Ishigatsubo Y, Tamura T
Organizer
Cytokines 2013
Place of Presentation
San Francisco, The United States of America
Related Report
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[Presentation] The IRF8-KLF4 transcription factor cascade is essential for the development of monocytes
Author(s)
Kurotaki D, Osato N, Nishiyama A, Yamamoto M, Ban T, Sato H, Nakabayashi J, Umehara M, Nakazawa M, Miyake N, Matsumoto N, Ozato K, Tamura T
Organizer
Cytokines 2013
Place of Presentation
San Francisco, The United States of America
Related Report
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[Presentation] 好塩基球分化の謎に迫る -転写因子IRF8の役割-
Author(s)
佐々木悠, 黒滝大翼, 大里直樹, 佐々木泉, 金田智香, 西山 晃, 改正恒康, 油谷浩幸, Herbert C. Morse III, Keiko Ozato, 田村智彦
Organizer
第15回神奈川血液・免疫フォーラム
Place of Presentation
新横浜プリンスホテル(神奈川県)
Related Report
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[Presentation] IRF8 inhibits the activity of C/EBPα to restrain the neutrophil differentiation program in monocyte-dendritic cell progenitors
Author(s)
Kurotaki D., Yamamoto M., Nishiyama A., Uno K., Ichino M., Sasaki H., Ban T., Yoshinari M., Nakazawa M., Ozato K., Tamura T
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸国際会議場(兵庫県)
Related Report
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[Presentation] The transcription factor IRF8 is a key transcription factor for basophil development
Author(s)
Kurotaki D, Sasaki H, Osato N, Sasaki I, Kaneda C, Sato H, Nishiyama A, Kaisho T, Aburatani H, Morse HC 3rd, Ozato K, Tamura T
Organizer
55th American Society of Hematology Annual Meeting and Exposition
Place of Presentation
New Orleans, The United States of America
Related Report
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