The search for myokines in mouse model of hypertrophy.

• Project/Area Number: 24790330
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Fujita Health University
• Principal Investigator: NAKATANI Masashi 藤田保健衛生大学, 総合医科学研究所, 助教 (00421264)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: マイオスタチン / プロテオミクス / 骨格筋 / 分泌因子 / 筋肥大 / 肥満

Outline of Final Research Achievements

Myostatin is a potent negative regulator of skeletal muscle. In this study, we find the myokines from myostatin deficient mouse. Muscle and serum from MSTN(-/-) mouse were purified any methods. Muscle purified was difficult, because muscle was including any actin and myosin. Serum from MSTN(-/-) was purified two affinity columns. The result of purified, we found a bone related molecule. The femur of Bone mineral density in MSTN(-/-) mouse was increased.

Research Products

• [Journal Article] Identification and characterization of PDGFRα mesenchymal progenitors in human skeletal muscle. (2014)
  • Author(s): Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Yamada H, Nishino I, Hamada Y, Tsuchida K.
  • Journal Title: Cell Death Disease Volume: 5 Issue: 4 Pages: 1186-1186
  • DOI: 10.1038/cddis.2014.161
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Myostatin signaling regulates Akt activity via the regulation of miR-486 expression. (2014)
  • Author(s): Hitachi K, Nakatani M, Tsuchida K.
  • Journal Title: Int J Biochem Cell Biol Volume: 47 Pages: 93-103
  • DOI: 10.1016/j.biocel.2013.12.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification and characterization of PDGFRα(+) mesenchymal progenitors in human skeletal muscle. (2014)
  • Author(s): A Uezumi, S Fukada, N Yamamoto, M Ikemoto-Uezumi, M Nakatani, M Morita, A Yamaguchi, H Yamada, I Nishino, Y Hamada and K Tsuchida
  • Journal Title: Cell Death and Disease Volume: 5 Pages: 1186-1186
  • Peer Reviewed
• [Journal Article] Myostatin signaling regulates Akt activity via the regulation of miR-486 expression. (2014)
  • Author(s): K Hitachi, M Nakatani, K Tsuchida
  • Journal Title: The International Journal of Biochemistry & Cell Biology Volume: 47 Pages: 93-103
  • Peer Reviewed
• [Presentation] 骨格筋分泌因子の探索 (2014)
  • Author(s): 中谷直史
  • Organizer: 日本肥満学会
  • Place of Presentation: 宮崎県 宮崎市 シーガイアコンベンションセンター
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] 骨格筋分泌因子の探索 (2012)
  • Author(s): 中谷直史
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: ホテルグランヴィア京都
• [Remarks] 藤田保健衛生大学総合医科学研究所難病治療学部門
  • URL: http://www.fujita-hu.ac.jp/~nanbyou/nanbyou/Top.html