pathophysiological analysis of autophagic substrate Nbr1.

• Project/Area Number: 24790332
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: SOU Yu-shin 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 研究員 (60576221)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: オートファジー / Nbr1 / nbr1 / p62

Research Abstract

To investigate a physiology of the Nbr1 metabolism, accumulation of Nbr1 and the affect to the liver, we created liver specific Atg7 and Nbr1-double deficient mice, and performed biochemical and morphological analysis.In liver specific Nbr1 knockout mice, the abnormality is not recognized. Simultaneous loss of Atg7 and Nbr1 in the liver cause hepatomegaly and liver dysfunction. It is shown that Loss of Nbr1 has few impacts on liver pathology in Atg7-deficient livers.
Further analysis is needed to clarify the pathophysiological role of Nbr1 in Atg7-p62 double knockout tissues.

Research Products

• [Journal Article] Phosphorylation of p62 activates the Keap1 - Nrf2 pathway during selective autophagy (2014)
  • Author(s): ①Ichimura Y, Waguri S, Sou YS, Kageyama S, Hasegawa J, Ishimura R, Saito T, Yang Y, Kouno T, Fukutomi T, Hoshii T, Hirao A, Takagi K, Mizushima T, Motohashi H, Lee MS, Yoshimori T, Tanaka K, Yamamoto M, Komatsu M
  • Journal Title: Mol Cell Volume: 51 Pages: 618-31
  • Peer Reviewed
• [Journal Article] LC3B is indispensable for selective autophagy of p62 but not basal autophagy (2014)
  • Author(s): ②Maruyama Y, Sou YS, Kageyama S, Takahashi T, Ueno T, Tanaka K, Komatsu M, Ichimura Y
  • Journal Title: Biochem Biophys Res Commun Volume: 446 Pages: 309-15
  • Peer Reviewed
• [Journal Article] A cluster of thin tubular structures mediates transformation of the endoplasmic reticulum to autophagic isolation membrane (2014)
  • Author(s): ③Uemura T, Yamamoto M, Kametaka A, Sou YS, Yabashi A, Yamada A, Annoh H, Kametaka S, Komatsu M, Waguri S
  • Journal Title: Mol Cell Biol Volume: 34 Pages: 1695-706
  • Peer Reviewed
• [Journal Article] LC3B is indispensable for selective autophagy of p62 but not basal autophagy. (2014)
  • Author(s): Maruyama Y, Sou YS, Kageyama S, Takahashi T, Ueno T, Tanaka K, Komatsu M, Ichimura Y.
  • Journal Title: Biochem Biophys Res Commun. Volume: 446 Issue: 1 Pages: 309-315
  • DOI: 10.1016/j.bbrc.2014.02.093
  • Peer Reviewed
• [Journal Article] Phosphorylation of p62 activates the Keap1-Nrf2 pathway during selective autophagy. (2013)
  • Author(s): Ichimura, Y., Waguri, S., Sou, Y., Kageyama, S., Hasegawa, J., Ishimura, R., Saito, T., Yang, Y., Kouno, T., Fukutomi, T., Hoshii, T., Atsushi, H., Takagi, K., Mizushima, T., Motohashi, H., Lee, M-S., Yoshimori, T., Tanaka, K., Yamamoto, M., and Komatsu, K.
  • Journal Title: Mol Cell Volume: 51 Issue: 5 Pages: 618-631
  • DOI: 10.1016/j.molcel.2013.08.003
  • Peer Reviewed / Open Access
• [Presentation] オートファジー選択的基質p62およびNbr1の病態生理的意義 (2013)
  • Author(s): 曽友深,和栗聡,田中啓二,小松雅明
  • Organizer: 第1回オートファジー班会議(ポスター発表)
  • Year and Date: 2013-12-20
• [Presentation] Phenotypic analysis of liver specific Atg7 Nbr1 - double knockout mice (2012)
  • Author(s): ②Yu-shin Sou, Satoshi Waguri, Keiji Tanaka, and Masaaki Komatsu
  • Organizer: 6th International symposium on Autophagy(ポスター発表)
  • Year and Date: 2012-10-28
• [Presentation] Phenotypic analysis of liver specific Atg7 Nbr1-double knockout mice (2012)
  • Author(s): Yu-shin Sou, Satoshi Waguri, Keiji Tanaka and Masaaki Komatsu
  • Organizer: 6th International Symposium on Autophagy 2012
  • Place of Presentation: 沖縄 ブセナテラス
• [Presentation] オートファジー選択的基質による凝集体形成制御機構
  • Author(s): 曽 友深、和栗 聡、 Caroline A Whitehouse、小松 雅明
  • Organizer: 第２３回日本生体防御学会学術総会
  • Place of Presentation: 東京 品川区総合区民会館
  • Invited