Evaluation of a role of SIK3 on immune system and screening of novel anti-inflammatory molecules

• Project/Area Number: 24790333
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: SANOSAKA Masato 独立行政法人医薬基盤研究所, 創薬基盤研究部, 特任研究員 (30510515)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 分子病態学 / 代謝学 / プロテインキナーゼ / SIK / 炎症

Research Abstract

SIK3-KO mice exhibit excessive liver injury accompanied with the secretion of pro-inflammatory molecules. This observation is suggested the activation of macrophages. In order to discover a novel strategy to control an inflammation, we evaluated the properties of macrophages prepared from SIK3-KO mice. SIK3-KO macrophages secrete the specific inflammatory molecules compared to wild type mice.
Furthermore, we identified pterosin as a SIK3-inhibitor. Pterosin inhibited inflammation of macrophages and it was suggested that pterosin is a new anti-inflammatory molecules.

Research Products

• [Journal Article] Involvement of SIK3 in glucose and lipid homeostasis in mice (2012)
  • Author(s): ①Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, Takikawa H, Fukusato T, Koura M, Nish M, Hamsten A, Silveira A, Bertorello AM, Kitagawa K, Nagaoka Y, Kawahara H, Tomonaga T, Naka T, Ikegawa S, Tsumaki N, Matsuda J, Takemori H
  • Journal Title: PLoS One Volume: 7(5)
  • Peer Reviewed
• [Journal Article] Involvement of SIK3 in Glucose and Lipid Homeostasis in Mice (2012)
  • Author(s): Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, et al.
  • Journal Title: PLoS One Volume: 7 Issue: 5 Pages: e37803-e37803
  • DOI: 10.1371/journal.pone.0037803
  • Peer Reviewed
• [Presentation] SIK3 regulates pro-inflammatory cyto - kine expression in mouse macrophages (2013)
  • Author(s): Masato Sanosaka, Minoru Fujimoto, Tomoharu Ohkawara, Yumi Ito, Ayako Kumagai, Tetsuji Naka, Hiroshi Takemori
  • Organizer: The 2013 ASCB Annual Meeting
  • Place of Presentation: The American Society for Cell Biology
  • Year and Date: 2013-12-17
• [Presentation] SIK3 regulates pro - inflammatory cyto - kine expression in mouse macrophages (2013)
  • Author(s): Masato Sanosaka, Minoru Fujimoto, Tomoharu Ohkawara, Yumi Ito, Ayako Kumagai, Tetsuji Naka, Hiroshi Takemori
  • Organizer: International Symposium on Transcription and Metabolism
  • Year and Date: 2013-11-12
• [Presentation] SIK3 regulates pro- inflammatory cytokine expression in mouse macrophages (2013)
  • Author(s): Masato Sanosaka, Minoru Fujimoto, Tomoharu Ohkawara, Yumi Ito, Ayako Kumagai, Tetsuji Naka, Hiroshi Takemori
  • Organizer: The 2013 ASCB Annual Meeting, The American Society for Cell Biology
  • Place of Presentation: New Orleans, LA, USA
• [Presentation] SIK3 regulates pro- inflammatory cytokine expression in mouse macrophages (2013)
  • Author(s): Masato Sanosaka, Minoru Fujimoto, Tomoharu Ohkawara, Yumi Ito, Ayako Kumagai, Tetsuji Naka, Hiroshi Takemori
  • Organizer: International Symposium on Transcription and Metabolism
  • Place of Presentation: Awajishima, Hyogo, JAPAN
• [Presentation] SIK3はマクロファージにおける炎症性サイトカインの発現を制御する (2012)
  • Author(s): 佐野坂真人、伊東裕美、藤本穣、大河原知治、仲哲治、竹森洋
  • Organizer: 第85回生化学会大会
  • Year and Date: 2012-12-16
• [Presentation] SIK3はマクロファージにおける炎症性サイトカインの発現を制御する (2012)
  • Author(s): 佐野坂 真人, 伊東 祐美, 藤本 穣, 大河原 知治, 仲 哲治, 竹森 洋
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡