| Project/Area Number |
24790348
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IKEMURA Masako 東京大学, 医学部附属病院, 助教 (80524553)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | パーキンソン病 / レビー小体病 / 皮膚生検 / α-シヌクレイン / レヴィ小体病 / αシヌクレイン / リン酸化αシヌクレイン / PLA |
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| Research Abstract |
Lewy bodies (LBs) containing alpha-synuclein are the hallmark lesions of Lewy body disease, including Parkinson disease (PD), PD with dementia, dementia with LBs and pure autonomic failure. Since protease treatment is known to enhance the immunoreactivity of abnormal alpha-synuclein, we immunohistochemically examined the abdominal skin tissue in LBD using anti-alpha-synuclein antibody with protease treatment. Protease-resistant alpha-synuclein is depositd in nerve fascicles mainly around blood vessels in LBD, but not in control subjects. The sensitivity of the positive skin immunoreactivity was 57.7% in subclinical PD, 88.2% in PD and PD with dementia and 53.8% in dementia with LBs. The sensitivity is higher than that using anti-phosphorylated alpha-synuclein antibody. Skin biopsy with ant-alpha-synuclein antibody may have diagnostic application in cases of LBD.
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