Molecular pathological approach to follicular lymphoma stem-like cells

• Project/Area Number: 24790350
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Okayama University
• Principal Investigator: TAKATA Katsuyoshi 岡山大学, 医歯(薬)学総合研究科, 講師 (90580259)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 悪性リンパ腫 / 濾胞性リンパ腫 / 幹細胞様集団 / 治療抵抗性 / 幹細胞 / なし

Outline of Final Research Achievements

We investigated several stem-like phenotype markers in follicular lymphoma patient samples, and some minor population was positive for these stem-like markers. These cells harbored t(14;18) by microdissection study. Furthermore, some minor stem-like positive cells presented in human derived B-cell lymphoma cell lines and these minor populations had treatment resistance and strong tumorigenic potential by in vitro and in vivo assay.

Research Products

• [Journal Article] Detection of T-cell receptor γ gene rearrangement in paraffin-embedded T or natural killer/T-cell lymphoma samples using the BIOMED-2 protocol. (2014)
  • Author(s): Miyata-Takata T, Takata K, Yamanouchi S, Sato Y, Harada M, Oka T, Tanaka T, Maeda Y, Tanimoto M, Yoshino T.
  • Journal Title: Leuk Lymphoma. Volume: 55 Issue: 9 Pages: 2161-2164
  • DOI: 10.3109/10428194.2013.871634
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Elevated soluble IL-2 receptor levels correlate with tumor bulk of follicular lymphomas with intestinal involvement. (2014)
  • Author(s): Iwamuro M, Shinagawa K, Okada H, Takata K, Yoshino T, Yamamoto K.
  • Journal Title: Clin Biochem Volume: 47 Issue: 3 Pages: 191-195
  • DOI: 10.1016/j.clinbiochem.2013.11.026
  • Peer Reviewed
• [Journal Article] Diagnostic accuracy of endoscopic biopsies for the diagnosis of gastrointestinal follicular lymphoma: a clinicopathologic study of 48 patients. (2014)
  • Author(s): Iwamuro M, Okada H, Takata K, Nose S, Miyatani K, Yoshino T, Yamamoto K.
  • Journal Title: Ann Diagn Pathol. Volume: 18 Issue: 2 Pages: 99-103
  • DOI: 10.1016/j.anndiagpath.2013.12.006
  • Peer Reviewed
• [Journal Article] Duodenal follicular lymphoma: Comprehensive gene expression analysis with insights into pathogenesis. (2014)
  • Author(s): Takata K, Tanino M, Ennishi D, Tari A, Sato Y, Okada H, Maeda Y, Goto N, Araki H, Harada M, Ando M, Iwamuro M, Tanimoto M, Yamamoto K, Gascoyne RD, Yoshino T.
  • Journal Title: Cancer Sci Volume: 6 Issue: 5 Pages: 608-615
  • DOI: 10.1111/cas.12392
  • Peer Reviewed
• [Journal Article] Low-grade B-cell lymphoma presenting primarily in the bone marrow. (2014)
  • Author(s): Iwatani K, Takata K, Sato Y, Miyata-Takata T, Iwaki N, Cui W, Sawada-Kitamura S, Sonobe H, Tamura M, Saito K, Miyatani K, Yamasaki R, Yamadori I, Fujii N, Terasaki Y, Maeda Y, Tanimoto M, Nakamura N, Yoshino T.
  • Journal Title: Hum Pathol Volume: 26 Issue: 7 Pages: 1379-1387
  • DOI: 10.1016/j.humpath.2014.02.010
  • NAID: 120005473076
  • Peer Reviewed
• [Journal Article] Clinicopathological analysis of 17 primary cutaneous T-cell lymphoma of theγδphenotype from Japan (2014)
  • Author(s): Takahashi Y, Takata K, Kato S, Sato Y, Asano N, Ogino T, Hashimoto K, Tashiro Y, Takeuchi S, Masunari T, Hiramatsu Y, Maeda Y, Tanimoto M, Yoshino T
  • Journal Title: Cancer Sci Volume: (Epub ahead of print) Issue: 7 Pages: 912-923
  • DOI: 10.1111/cas.12439
  • Peer Reviewed
• [Journal Article] Pathology of follicular lymphoma. (2014)
  • Author(s): Takata K, Miyata-Takata T, Sato Y, Yoshino T.
  • Journal Title: J Clin Exp Hematop. Volume: 54 Pages: 3-9
  • Peer Reviewed
• [Journal Article] Prognostic biomarkers in patients with localized natural killer/T-cell lymphoma treated with concurrent chemoradiotherapy. (2014)
  • Author(s): Yamaguchi M, Takata K, Yoshino T, Ishizuka N, Oguchi M, Kobayashi Y, Isobe Y, Ishizawa K, Kubota N, Itoh K, Usui N, Miyazaki K, Wasada I, Nakamura S, Matsuno Y, Oshimi K, Kinoshita T, Tsukasaki K, Tobinai K.
  • Journal Title: Cancer Sci Volume: 105 Issue: 11 Pages: 1435-1441
  • DOI: 10.1111/cas.12526
  • Peer Reviewed
• [Journal Article] Immunoglobulin expressions are only associated with MCPyV-positive Merkel cell carcinomas but not with MCPyV-negative ones: comparison of prognosis. (2014)
  • Author(s): Murakami I, Takata K, Matsushita M, Nonaka D, Iwasaki T, Kuwamoto S, Kato M, Mohri T, Nagata K, Kitamura Y, Yoshino T, Hayashi K.
  • Journal Title: Am J Surg Pathol. Volume: 38 Issue: 12 Pages: 1627-1635
  • DOI: 10.1097/pas.0000000000000279
  • Peer Reviewed
• [Journal Article] Detection of T-cell receptor gamma gene rearrangement in paraffin-embedded T or natural killer/T-cell lymphoma samples using the BIOMED-2 protocol. (2014)
  • Author(s): Miyata-Takata T, Takata K (corresponding author), Yamanouchi S, Sato Y, Harada M, Oka T, Tanaka T, Maeda Y, Tanimoto M, Yoshino T.
  • Journal Title: Leukemia & lymphoma Volume: 24
  • Peer Reviewed
• [Journal Article] Epstein-Barr Virus-infected Cells in IgG4-related Lymphadenopathy With Comparison With Extranodal IgG4-related Disease. (2014)
  • Author(s): Takeuchi M, Sato Y, Yasui H, Ozawa H, Ohno K, Takata K, Gion Y, Orita Y, Tachibana T, Itoh T, Asano N, Nakamura S, Swerdlow SH, Yoshino T.
  • Journal Title: Am J Surg Pathol. Volume: 3
  • Peer Reviewed
• [Journal Article] In aggressive variants of non-Hodgkin lymphomas, Ezh2 is strongly expressed and polycomb repressive complex PRC1.4 dominates over PRC1.2. (2013)
  • Author(s): Abd Al Kader L, Oka T, Takata K, Sun X, Sato H, Murakami I, Toji T, Manabe A, Kimura H, Yoshino T.
  • Journal Title: Virchows Arch. Volume: 463 Issue: 5 Pages: 697-711
  • DOI: 10.1007/s00428-013-1428-y
  • NAID: 120005359111
  • Peer Reviewed
• [Journal Article] A20 (TNFAIP3) Deletion in Epstein-Barr Virus-Associated Lymphoproliferative Disorders/Lymphomas. (2013)
  • Author(s): Ando M, Sato Y, Takata K, Nomoto J, Nakamura S, Ohshima K, Takeuchi T, Orita Y, Kobayashi Y, Yoshino T.
  • Journal Title: PLoS One Volume: 8 Issue: 2 Pages: e56741-e56741
  • DOI: 10.1371/journal.pone.0056741
  • NAID: 120005359115
  • Peer Reviewed
• [Journal Article] Distinct morphologic, phenotypic, and clinical-course characteristics of indolent peripheral T-cell lymphoma (2013)
  • Author(s): Hayashi E, Takata K, Sato Y, Tashiro Y, Tachiyama Y, Sawada-Kitamura S, Hiramatsu Y, Sugiguchi S, Nose S, Hirokawa M, Ando M, Abd Alkader L, Maeda Y, Tanimoto M, Yoshino T
  • Journal Title: Hum Pathol Volume: 44 Issue: 9 Pages: 1927-1936
  • DOI: 10.1016/j.humpath.2013.03.002
  • NAID: 120005359108
  • Peer Reviewed
• [Journal Article] De novo CD5-positive diffuse large B-cell lymphomas show high specificity for cyclin D2 expression. (2013)
  • Author(s): Igawa T, Sato Y, Takata K, Iwaki N, Tanaka T, Asano N, Maeda Y, Orita Y, Nakamura N, Nakamura S, Yoshino T.
  • Journal Title: Diagnostic pathology Volume: 8 Issue: 1 Pages: 81-81
  • DOI: 10.1186/1746-1596-8-81
  • Peer Reviewed
• [Journal Article] Primary intestinal follicular lymphoma: How to identify follicular lymphoma by routine endoscopy. (2013)
  • Author(s): wamuro M, Kawai Y, Takata K, Kawano S, Yoshino T, Okada H, Yamamoto K.
  • Journal Title: World journal of gastrointestinal endoscopy Volume: 5 Issue: 1 Pages: 34-38
  • DOI: 10.4253/wjge.v5.i1.34
  • Peer Reviewed
• [Journal Article] Over-expression of BACH2 is related to ongoing somatic hypermutation of the immunoglobulin heavy chain gene variable region of de novo diffuse large B-cell lymphoma. (2013)
  • Author(s): Kikuchi T, Tokunaka M, Kikuti YY, Carreras J, Ogura G, Takekoshi S, Kojima M, Ando K, Hashimoto Y, Abe M, Takata K, Yoshino T, Muto A, Igarashi K, Nakamura N.
  • Journal Title: Pathol Int Volume: 63 Issue: 7 Pages: 339-344
  • DOI: 10.1111/pin.12076
  • Peer Reviewed
• [Journal Article] Clinicopathologic analysis of IgG4-related skin disease. (2013)
  • Author(s): Sato Y, Takeuchi M, Takata K, Ohno K, Iwaki N, Orita Y, Goto N, Hida AI, Iwamoto T, Asano N, Ito T, Hanakawa H, Yanai H, Yoshino T.
  • Journal Title: Mod Pathol. Volume: 26 Issue: 4 Pages: 523-532
  • DOI: 10.1038/modpathol.2012.196
  • Peer Reviewed
• [Journal Article] Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma. (2013)
  • Author(s): Shimoura Y, Sato Y, Takata K, Orita Y, Nakamura S, Mano S, Yoshino T.
  • Journal Title: Acta cytologica Volume: 57 Issue: 1 Pages: 84-90
  • DOI: 10.1159/000342475
  • Peer Reviewed
• [Journal Article] Duodenal follicular lymphoma lacks AID but expresses BACH2 and has memory B-cell characteristics (2013)
  • Author(s): Takata K, Sato Y, Nakamura N, Tokunaka M, Miki Y, Yukie Kikuti Y, Igarashi K, Ito E, Harigae H, Kato S, Hayashi E, Oka T, Hoshii Y, Tari A, Okada H, Mohamad AA, Maeda Y, Tanimoto M, Kinoshita T, Yoshino T
  • Journal Title: Mod Pathol Volume: 26(1) Issue: 1 Pages: 22-31
  • DOI: 10.1038/modpathol.2012.127
  • NAID: 130004505772
  • Peer Reviewed
• [Journal Article] Clinicopathologic analysis of localized nasal/paranasal diffuse large B-cell lymphoma. (2013)
  • Author(s): Toda H, Sato Y, Takata K, Orita Y, Asano N, Yoshino T.
  • Journal Title: PLoS One Volume: 8 Issue: 2 Pages: e57677-e57677
  • DOI: 10.1371/journal.pone.0057677
  • NAID: 120005324797
  • Peer Reviewed
• [Journal Article] A20(TNFAIP3) deletion in Epstein-Barr virus-associated lymphoprotiferative disorders/lymphomas (2013)
  • Author(s): Ando M, Sato Y, Takata K et al.
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Clinicopathologic analysis of localized nasal/paranasal diffuse large B-cell lymphoma (2013)
  • Author(s): Toda H, Sato Y, Takata K et al.
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Distinct morphologic, phenotypic, and clinical-course characteristics of indolent peripheral T-cell lymphoma (2013)
  • Author(s): Hayashi E, Takata K, Sato Y et al.
  • Journal Title: Human Pathology Volume: 未定
  • Peer Reviewed
• [Journal Article] Primary cutaneous CD30 positive T-cell lymphoproliferative disorders with aberrant expression of PAX5: report of three cases (2012)
  • Author(s): Hagiwara M, Tomita A, Takata K et al.
  • Journal Title: Pathology International Volume: 62 Pages: 264-70
  • Peer Reviewed
• [Journal Article] Diagnostic role of 18F-fluorodeoxygluocose positron emission tomography for follicular lymphoma with gastrointestinal involvement (2012)
  • Author(s): Iwamuro M, Okada H, Takata K et al.
  • Journal Title: World J Gastroenterology Volume: 18 Pages: 6427-36
  • Peer Reviewed
• [Presentation] Prognostic biomarkers in patients with localized NK/T-cell lymphoma treated with RT-DeVIC (2014)
  • Author(s): Takata K, Yamaguchi M, Yoshino T, Ishizuka N, Oguchi M, Kobayashi Y, Isobe Y, Ishizawa K, Kubota N, Itoh K, Usui N, Miyazaki K.
  • Organizer: 日本血液学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] Mantle cell lymphoma morphologically mimicking multicentric Castleman's disease (2014)
  • Author(s): Katsuyoshi Takata and Tadashi Yoshino
  • Organizer: International Academy of Pathology
  • Place of Presentation: Thailand
  • Year and Date: 2014-10-05 – 2014-10-10
  • Invited
• [Presentation] Intestinal follicular lymphoma (2014)
  • Author(s): 高田尚良、吉野 正
  • Organizer: 日本リンパ網内系学会
  • Place of Presentation: 山形
  • Year and Date: 2014-06-19 – 2014-06-21
  • Invited
• [Presentation] 腸管悪性リンパ腫の特徴および病理診断 (2014)
  • Author(s): 高田尚良
  • Organizer: 日本リンパ網内系学会
  • Place of Presentation: 山形
  • Year and Date: 2014-06-19 – 2014-06-21
  • Invited
• [Presentation] リンパ芽球性リンパ腫における可溶性インターロイキン２受容体とCD25発現の関連性についての検討 (2014)
  • Author(s): 都地友紘、高田尚良、佐藤康晴、吉野 正
  • Organizer: 日本病理学会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 消化管ＤＬＢＣＬの病理診断 (2013)
  • Author(s): 高田尚良、吉野 正
  • Organizer: 日本リンパ網内系学会
  • Place of Presentation: 京都
  • Invited
• [Presentation] Follicular lymphoma in situの2例 (2013)
  • Author(s): 原田 舞、高田尚良、小田和歌子、佐藤康晴、吉野 正
  • Organizer: 日本リンパ網内系学会
  • Place of Presentation: 京都
• [Presentation] 消化管濾胞性リンパ腫の最新知見 (2013)
  • Author(s): 高田尚良、吉野 正
  • Organizer: 日本病理学会
  • Place of Presentation: 札幌
  • Invited
• [Presentation] 低悪性度抹消型Ｔ細胞性リンパ腫非特異型のＴ細胞のサブタイプによる臨床学的検討 (2013)
  • Author(s): 林 詠子、高田尚良、北村星子、園部 宏、田村麻衣子、齋藤勝彦、宮谷克也、山崎理恵、山鳥一郎、中村直哉、吉野 正
  • Organizer: 日本病理学会
  • Place of Presentation: 札幌
• [Presentation] 骨髄原発低悪性度Ｂ細胞性リンパ腫14例の検討 (2013)
  • Author(s): 岩谷佳代子、高田尚良、吉野 正
  • Organizer: 日本病理学会
  • Place of Presentation: 札幌
• [Presentation] 皮膚原発γδＴ細胞リンパ腫の臨床病理学的解析 (2013)
  • Author(s): 高田尚良、高橋友香、加藤省一、谷本光音、吉野 正
  • Organizer: 日本癌学会
  • Place of Presentation: 横浜
• [Presentation] 十二指腸濾胞性リンパ腫の発症機構とエピジェネティクス解析
  • Author(s): 高田尚良、岡 剛史、安藤翠、都地友紘、佐藤康晴、中村直哉、吉野 正
  • Organizer: 第１０１回日本病理学会
  • Place of Presentation: 東京
• [Presentation] 十二指腸濾胞性リンパ腫のメチル化と発症機構の解析
  • Author(s): 高田尚良、岡 剛史、中村直哉、吉野 正
  • Organizer: 第７１回日本癌学会
  • Place of Presentation: 札幌
• [Presentation] 消化管DLBCLの病理診断
  • Author(s): 高田尚良、吉野 正
  • Organizer: 日本リンパ網内系学会
  • Place of Presentation: 京都
  • Invited