The function of glycine N-methyltransferase in the proliferation of renal cell carcinoma

• Project/Area Number: 24790351
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Kyushu University
• Principal Investigator: SONG YooHyun 九州大学, 医学(系)研究科(研究院), 研究員 (10586746)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed(Fiscal Year 2014)
• Budget Amount: ¥2,210,000 (Direct Cost : ¥1,700,000、Indirect Cost : ¥510,000); Fiscal Year 2014 : ¥780,000 (Direct Cost : ¥600,000、Indirect Cost : ¥180,000); Fiscal Year 2013 : ¥780,000 (Direct Cost : ¥600,000、Indirect Cost : ¥180,000); Fiscal Year 2012 : ¥650,000 (Direct Cost : ¥500,000、Indirect Cost : ¥150,000)
• Keywords: 腎癌 / GNMT

Outline of Final Research Achievements

We have reported that the GNMT gene acts as an oncogene. However, little is known about the specific function of GNMT in carcinogenesis and progression in renal cell carcinoma (RCC).
We performed Western blotting analysis using normal kidney cells and RCC cells. GNMT proteins were expressed in normal kidney cell and most RCC cells.In immunohistochemistry, GNMT protein was expressed in the cytoplasm of RCC cells. We found that the knockdown of GNMT expression induced apparently effect on RCC cell proliferation. Furthermore, GNMT knockdown in RCC cell induced prominent increases in the sub-G1 fraction, indicating cellular death. To investigate the mechanism of cellular death by GNMT knockdown, Western blotting using an antibody against cleaved PARP, which indicates the presence of degradation products from the caspase cascade, was employed. GNMT knockdown induced cleavage of PARP in RCC cells.
GNMT may play an important role in promoting cell growth via the regulation of apoptosis of RCC.

Research Products

• [Journal Article] Dual inhibition of EZH2 and EZH1 sensitizes PRC2-dependent tumors to proteasome inhibition. (2017)
  • Author(s): Ola Rizq, Naoya Mimura, Motohiko Oshima, Atsunori Saraya, Shuhei Koide, Yuko Kato, Kazumasa Aoyama, Yaeko Nakajima-Takagi, Changshan Wang, Tetsuhiro Chiba, Anqi Ma, Jian Jin, Tohru Iseki, Chiaki Nakaseko, and Atsushi Iwama
  • Journal Title: Clinical Cancer Research Volume : 23(16) Pages : 4817-4830
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] スニチニブの有害事象と投与 スケジュールに関する検討 (2014)
  • Author(s): 宋裕賢 立神勝則 内藤誠二
  • Journal Title: 泌尿器外科 Volume : 27 Pages : 46-47
  • Peer Reviewed
• [Journal Article] Interaction between docetaxel resistance and castration resistance in prostate cancer: Implications of Twist1, YB-1, and androgen receptor (2013)
  • Author(s): Shiota M, Kashiwagi E, Yokomizo A, Takeuchi A, Dejima T, Song Y, Tatsugami K, Inokuchi J, Uchiumi T, Naito S
  • Journal Title: Prostate Volume : 73 Pages : 1336-44
  • DOI: 10.1002/pros.22681
  • Peer Reviewed
• [Journal Article] Human heterochromatin protein 1 isoforms regulate androgen receptor signaling in prostate cancer. (2013)
  • Author(s): Itsumi M, Shiota M, Yokomizo A, Kashiwagi E, Takeuchi A, Tatsugami K, Inokuchi J, Song Y, Uchiumi T, Naito S
  • Journal Title: J Mol Endocrinol Volume : 50 Pages : 401-9
  • DOI: 10.1530/jme-13-0024
  • Peer Reviewed
• [Journal Article] Twist1 and Y-box-binding protein-1 are potential prognostic factors in bladder cancer. (2013)
  • Author(s): Song YH, Shiota M, Yokomizo A, Uchiumi T, Kiyoshima K, Kuroiwa K, Oda Y, Naito S.
  • Journal Title: Urologic Oncology Volume : 12 Pages : 1078-1439
  • DOI: 10.1016/j.urolonc.2012.11.003
  • Peer Reviewed
• [Journal Article] Human heterochromatin protein 1 isoforms regulate androgen receptor signaling in prostate cancer. (2013)
  • Author(s): Itsumi M, Shiota M, Yokomizo A, Kashiwagi E, Takeuchi A, Tatsugami K, Inokuchi J, Song Y, Uchiumi T, Naito S.
  • Journal Title: Journal of Molecular Endocrinology Volume : 不明
  • Peer Reviewed
• [Journal Article] Causes of diminished renal function in the affected kidney after partial nephrectomy. (2012)
  • Author(s): Tatsugami K, Tanaka S, Ohtsubo S, Inokuchi J, Yokomizo A, Kuroiwa K, Song YH, Naito S.
  • Journal Title: BJU international Volume : 110 Pages : 357-61
  • DOI: 10.1111/j.1464-410x.2012.011027.x
  • Peer Reviewed
• [Presentation] The function of glycine N-methyltransferase in the proliferation of renal cell carcinoma (2014)
  • Author(s): Yoo Hyun Song, Masaki Shiota, Akira Yokomizo, Katsunori Tatsugami, and Seiji Naito
  • Organizer: SOCIETE INTERNATIONALE D'UROLOGIE (SIU)
  • Place of Presentation: Glasgow, Scotland
  • Year and Date: 2014-10-12 – 2014-10-15
• [Presentation] Review of sunitinib adverse events associated with the schedules (2013)
  • Author(s): 宋 裕賢、立神勝則、内藤誠二
  • Organizer: 癌治療学会学術集会
  • Place of Presentation: 京都
• [Presentation] The important role of glycine N-methyltransferase (GNMT) in the proliferation of renal cell carcinoma (2013)
  • Author(s): Yoo Hyun Song, Masaki Shiota, Akira Yokomizo, Katsunori Tatsugami, Kentaro Kuroiwa, Yoshinao Oda, Takeshi Uchiumi and Seiji Naito
  • Organizer: EAU annual congress
  • Place of Presentation: Milano, Italy