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The function of glycine N-methyltransferase in the proliferation of renal cell carcinoma

Research Project

Project/Area Number 24790351
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Human pathology
Research InstitutionKyushu University

Principal Investigator

SONG YooHyun  九州大学, 医学(系)研究科(研究院), 研究員 (10586746)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords腎癌 / GNMT
Outline of Final Research Achievements

We have reported that the GNMT gene acts as an oncogene. However, little is known about the specific function of GNMT in carcinogenesis and progression in renal cell carcinoma (RCC).
We performed Western blotting analysis using normal kidney cells and RCC cells. GNMT proteins were expressed in normal kidney cell and most RCC cells.In immunohistochemistry, GNMT protein was expressed in the cytoplasm of RCC cells. We found that the knockdown of GNMT expression induced apparently effect on RCC cell proliferation. Furthermore, GNMT knockdown in RCC cell induced prominent increases in the sub-G1 fraction, indicating cellular death. To investigate the mechanism of cellular death by GNMT knockdown, Western blotting using an antibody against cleaved PARP, which indicates the presence of degradation products from the caspase cascade, was employed. GNMT knockdown induced cleavage of PARP in RCC cells.
GNMT may play an important role in promoting cell growth via the regulation of apoptosis of RCC.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (3 results)

  • [Journal Article] スニチニブの有害事象と投与 スケジュールに関する検討2014

    • Author(s)
      宋裕賢 立神勝則 内藤誠二
    • Journal Title

      泌尿器外科

      Volume: 27 Pages: 46-47

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutual regulation between Raf/MEK/ERK signaling and Y-box-binding protein-1 promotes prostate cancer progression2013

    • Author(s)
      Imada K, Shiota M, Kohashi K, Kuroiwa K, Song Y, Sugimoto M, Naito S, Oda Y
    • Journal Title

      Clin Cancer Res.

      Volume: 19 Pages: 4638-50

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interaction between docetaxel resistance and castration resistance in prostate cancer: Implications of Twist1, YB-1, and androgen receptor2013

    • Author(s)
      Shiota M, Kashiwagi E, Yokomizo A, Takeuchi A, Dejima T, Song Y, Tatsugami K, Inokuchi J, Uchiumi T, Naito S
    • Journal Title

      Prostate

      Volume: 73 Issue: 12 Pages: 1336-44

    • DOI

      10.1002/pros.22681

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human heterochromatin protein 1 isoforms regulate androgen receptor signaling in prostate cancer.2013

    • Author(s)
      Itsumi M, Shiota M, Yokomizo A, Kashiwagi E, Takeuchi A, Tatsugami K, Inokuchi J, Song Y, Uchiumi T, Naito S
    • Journal Title

      J Mol Endocrinol

      Volume: 50 Issue: 3 Pages: 401-9

    • DOI

      10.1530/jme-13-0024

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Twist1 and Y-box-binding protein-1 are potential prognostic factors in bladder cancer.2013

    • Author(s)
      Song YH, Shiota M, Yokomizo A, Uchiumi T, Kiyoshima K, Kuroiwa K, Oda Y, Naito S.
    • Journal Title

      Urologic Oncology

      Volume: 12 Issue: 1 Pages: 1078-1439

    • DOI

      10.1016/j.urolonc.2012.11.003

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human heterochromatin protein 1 isoforms regulate androgen receptor signaling in prostate cancer.2013

    • Author(s)
      Itsumi M, Shiota M, Yokomizo A, Kashiwagi E, Takeuchi A, Tatsugami K, Inokuchi J, Song Y, Uchiumi T, Naito S.
    • Journal Title

      Journal of Molecular Endocrinology

      Volume: 不明

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Causes of diminished renal function in the affected kidney after partial nephrectomy.2012

    • Author(s)
      Tatsugami K, Tanaka S, Ohtsubo S, Inokuchi J, Yokomizo A, Kuroiwa K, Song YH, Naito S.
    • Journal Title

      BJU international

      Volume: 110 Issue: 8b Pages: 357-61

    • DOI

      10.1111/j.1464-410x.2012.011027.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] The function of glycine N-methyltransferase in the proliferation of renal cell carcinoma2014

    • Author(s)
      Yoo Hyun Song, Masaki Shiota, Akira Yokomizo, Katsunori Tatsugami, and Seiji Naito
    • Organizer
      SOCIETE INTERNATIONALE D'UROLOGIE (SIU)
    • Place of Presentation
      Glasgow, Scotland
    • Year and Date
      2014-10-12 – 2014-10-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Review of sunitinib adverse events associated with the schedules2013

    • Author(s)
      宋 裕賢、立神勝則、内藤誠二
    • Organizer
      癌治療学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] The important role of glycine N-methyltransferase (GNMT) in the proliferation of renal cell carcinoma2013

    • Author(s)
      Yoo Hyun Song, Masaki Shiota, Akira Yokomizo, Katsunori Tatsugami, Kentaro Kuroiwa, Yoshinao Oda, Takeshi Uchiumi and Seiji Naito
    • Organizer
      EAU annual congress
    • Place of Presentation
      Milano, Italy
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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