Acquisition of novel bio-markers for cancers using cancer-specific exosomal proteins.
Project/Area Number |
24790360
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Human pathology
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Research Institution | Kitasato University |
Principal Investigator |
NAGASHIO Ryo 北里大学, 医療衛生学部, 講師 (40618568)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | エクソゾーム / ランダム免疫法 / 単クローン性抗体 / プロテオミクス / 組織診断マーカー / 血清診断マーカー / 自己抗体 |
Outline of Final Research Achievements |
There are few useful biomarkers for early diagnosis of lung cancer. Therefore most patients with lung cancer are at an advanced stage at the time of diagnosis. To obtain useful diagnostic markers for lung adenocarcinoma, we generated monoclonal antibodies using exosomal proteins from lung adenocarcinoma-derived A549 cells by employing the random immunization method. To evaluate the utility of these antibodies as differential diagnostic marker and/or sero-diagnostic marker for lung adenocarcinoma, we performed immunohistochemical study with tissue microarray and dotblot analysis with sera from lung cancer patients and normal controls. As the results, we successfully obtained the candidate biomarkers for diagnosis of lung adenocarcinomas.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Diagnostic and prognostic significances of MUC5B and TTF-1 expressions in resected non-small cell lung cancer.2015
Author(s)
Nagashio R, Ueda J, Ryuge S, Nakashima H, Jiang SX, Kobayashi M, Yanagita K, Katono K, Satoh Y, Masuda N, Murakumo Y, Hachimura K, Sato Y.
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 8649-8649
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Diagnostic Significance of MUC5B and TTF-1 Expression in Resected Non-Small Cell Lung Cancer2013
Author(s)
Ryo Nagashio, Junpei Ueda, Sho Minami, Makoto Kobayashi, Kengo Yanagita, Shinichiro Ryuge, Ken Katono, Hiroyasu Nakashima, Shi-Xu Jiang, Yukitoshi Satoh, Noriyuki Masuda, Yoshiki Murakumo, Yuichi Sato
Organizer
HUPO 12th Annual World Congress
Place of Presentation
Pacifico Yokohama (Yokohama)
Related Report
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