Do LAM cells express VEGF-D through the activation of mTORC1/ HIF-1a/ VEGF pathway?
| Project/Area Number |
24790363
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Juntendo University |
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Principal Investigator |
HAYASHI TAKUO 順天堂大学, 医学部, 非常勤助教 (70569128)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | リンパ脈管筋腫症 / TSC遺伝子 / リンパ管内皮細胞増殖因子 / 低酸素誘導因子 / mTORシグナル伝達系 / VEGF-D / HIF-1α / 人体病理 |
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| Research Abstract |
In the present study, we performed immunohistochemistry and western blot analysis to elucidate whether LAM cells express Vascular endothelial growth factor (VEGF)-D through the activation of mammalian target of rapamycin complex 1(mTORC1)/ Hypoxia Inducible Factor-1a (HIF-1a)/ VEGF pathway. LAM cells were immunopositive for p-70S6K, p-S6, p-4E-BP1, and VEGF-D in all patients examined. On the other hand, no nuclear expression of HIF-1a was detected in any LAM cells. Western blot analysis confirmed no expression of HIF-1a in LAM cells. Our results suggest that LAM cells expressed VEGF-D though mTORC1/HIF-1a/VEGF-independent pathway.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Galectin-4, a novel predictor for lymph node metastasis in lung adenocarcinoma2013
Author(s)
Hayashi T, Saito T, Fujimura T, Hara K, Takamochi K, Mitani K, Mineki R, Kazuno S, Oh S, Ueno T, Suzuki K, Yao T
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Journal Title
PLoS One
Volume: 10;8(12)
Related Report
Peer Reviewed
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[Journal Article] Uterine angiosarcoma associated with lymphangioleiomyomatosis in a patient with tuberous sclerosis complex : an autopsy case report with immunohistochemical and genetic analysis2013
Author(s)
Hayashi T, Koike K, Kumasaka T, Saito T, Mitani K, Terao Y, Ogishima D, Yao T, Takeda S, Takahashi K, Seyama K
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Journal Title
Hum Pathol
Volume: 43
Pages: 1777-84
Related Report
Peer Reviewed
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