| Project/Area Number |
24790367
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | in situハイブリダイゼーション法 / C型肝炎ウイルス / locked nucleic acid / ビオチンフリーチラミド増感法 / ヒト肝臓型HCV感染キメラマウス / HCV / in situ hybridization / AT tailing / HCV genotyping / nested RT-PCR |
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| Research Abstract |
We were successful to detect HCV-RNA in formalin-fixed, paraffin-embedded sections of liver by using high-sensitivity in situ hybridization (ISH) combined with LNA-modified oligonucleotide probe and biotin-free tyramide amplification system (CSAII). HCV-RNA was demonstrated in HCV-infected liver tissues from both chimeric mice and 9 (82%) of 11 patients with HCV infection. HCV-RNA was not identified chronic hepatitis B, fatty liver, autoimmune hepatitis, and hepatocellular carcinoma. Nested RT-PCR confirmed the genotype in 8 (73%) of 11 liver (type 1b: 6 lesions and type 2a: 2 lesions). HCV genotyping could be detected by nested RT-PCR, while high-sensitivity ISH technique occurred cross reaction among the HCV genotypes.
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