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Identification and characterization of osteoclast-derived coupling factor

Research Project

Project/Area Number 24790371
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Human pathology
Research InstitutionNational Center for Geriatrics and Gerontology

Principal Investigator

MATSUOKA Kazuhiko  独立行政法人国立長寿医療研究センター, 運動器疾患研究部, 流動研究員 (00581365)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords骨芽細胞 / 破骨細胞 / カップリング / 骨リモデリング / 細胞分解 / 細胞分化
Research Abstract

Bone remodeling is regulated by a coupling of resorption to subsequent formation, however, the "coupling factor" and underlying mechanism are not fully understood. I purified osteoblast-stimulating activity, as determined by ALP activity, from osteoclast CM through successive chromatography by monitoring the ALP activity, and identified complement component 3 (C3). The activity present in osteoclast CM was inhibited by a specific C3a receptor antagonist, SB290157. C3 gene expression in bone was increased in the high bone turnover states of ovariectomy (OVX) or a RANKL injection, and blocking the action of C3a with the daily administration of SB290157 resulted in the attenuation of bone formation elevated by OVX, and the exacerbation of bone loss. These results suggest that osteoclast-derived C3a functions in the relay from bone resorption to formation and may be a candidate for a coupling factor.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] Osteoclast-derived complement component 3a stimulates osteoblast differentiation2014

    • Author(s)
      Matsuoka K, Park K, Ito M, Ikeda K, Takeshita S
    • Journal Title

      J Bone Miner Res

      Volume: 29 Issue: 7 Pages: 1522-1530

    • DOI

      10.1002/jbmr.2187

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Osteoclast-secreted Complement Component 3a Stimulates Osteoblast Differentiation2014

    • Author(s)
      Kazuhiko Matsuoka
    • Organizer
      国内会議第8会Bone Research Seminar
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Osteoclast-secreted Complement Component 3a Stimulates Osteoblast Differentiation2013

    • Author(s)
      Matsuoka K, Ito M, Ikeda K, and Takeshita S
    • Organizer
      国際会議The American Society for Bone and Mineral Research Annual Meeting
    • Place of Presentation
      Baltimore, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Purification of a secretory product of osteoclasts that promotes osteoblast differentiation2012

    • Author(s)
      Kazuhiko Matsuoka, Kyoji Ikeda, and Sunao Takeshita
    • Organizer
      第85回日本生化学会大会合同大会20121200
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 破骨細胞が分泌する骨芽細胞分化促進因子の精製2012

    • Author(s)
      松岡和彦
    • Organizer
      日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Osteoclast-secreted Complement Component 3a Stimulates Osteoblast Differentiation

    • Author(s)
      Kazuhiko Matsuoka
    • Organizer
      American Society for Bone and Mineral Research
    • Place of Presentation
      Baltimore, Maryland , USA
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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