| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Wnt/β-catenin signaling is frequently activated in gastric cancers but it remains poorly understood how Wnt signaling contributes to the gastric carcinogenesis. In the present study, we investigated the effect of activation of Wnt signaling in gastric epithelial cells using doxycycline-inducible β-catenin mice. When we fed adult mice doxycycline for 5 days, cytoplasmic and nuclear β-catenin accumulation were observed in both fundic and pyloric glands of the stomach and the number of Ki-67-positive proliferating cells significantly increased in the isthmus and gastric pits with significant decrease in mucus production. Gene expression analysis revealed that β-catenin induction led to the up-regulations of gastric and intestinal epithelial stem cell markers. Our data indicate that Wnt activation maintains gastric epithelial cells in an undifferentiated and proliferative state.
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