| Project/Area Number |
24790390
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
TOMIKAWA Naoki 福島県立医科大学, 医学部, 講師 (80468587)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 再生医学 / 幹細胞 / 上皮細胞 / 上皮分化 / タイト結合 / 国際情報交換 |
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| Research Abstract |
We hypothesis that claudin-6 (Cldn6), tight junction protein, function as the coordinator in the differentiation from stem cells to epithelial cells. In mouse F9 stem cell lines and embryonic stem cells, the expression of Cldn6 increased other tight junction protein and induced the differentiation to mature epithelial cells. Moreover, the localization of Cldn6 to cell-cell adhesion, second extracellular domain of Cldn6, and 196 and 200 tyrosine residues in C-terminal intracellular domain of Cldn6 were essential for Cldn6-induced epithelial differentiation. In addition, Cldn6 interacted with src-family kinases (SFKs), and then activated SFKs phosphorylated 196 and 200 tyrosine residues in Cldn6. These results indicate that Cldn6 and SFKs cooperatively induced the signaling for epithelial differentiation.
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