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Regulatory mechanism of tumor cell proliferation by pluripotency-associated transcriptional repressor NACC1

Research Project

Project/Area Number 24790391
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionIwate Medical University

Principal Investigator

KASAI Shuya  岩手医科大学, 医学部, 研究員 (20609664)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsNACC1 / 乳癌 / 腫瘍増殖 / 腫瘍悪性化
Research Abstract

Elevated expression of NACC1 associates with tumor recurrence and growth of breast, ovarian and cervical cancers. To clarify the molecular mechanism of tumor growth acceleration by NACC1 overexpression in human malignancies, we determined whether NACC1 regulates beta-catenin/TCF pathway in breast cancer cell lines. In parallel, proteomic analysis of NACC1 interacting proteins was carried out. We identified NACC2, ZKSCAN1 and PLK1 as novel NACC1-intracting proteins. NACC1-PLK1 interaction was elevated in cells synchronized at metaphase compared to asynchronous cells. PLK1 expression and NACC1-PLK1 colocalization was observed specifically in metaphase, whereas NACC1 expression was observed in any cell cycle stages. In addition, NACC1 phosphorylation by PLK1 was elevated by metaphase synchronization.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] beta-catenin signaling induces CYP1A1 expression by disrupting adherens junctions in Caco-2 human colon carcinoma cells.2013

    • Author(s)
      Shuya Kasaia, Takanori Ishigaki, Ryo Takumi, Tohoru Kamimura, Hideaki Kikuchi,
    • Journal Title

      Biochim. Biophys. Acta,

      Volume: 1830 Issue: 3 Pages: 2509-2516

    • DOI

      10.1016/j.bbagen.2012.11.007

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcriptional and post-transcriptional regulation of βIII-tubulin protein expression in relation with cell cycle-dependent regulation of tumor cells2012

    • Author(s)
      Shibazaki M, Maesawa C, Akasaka K, Kasai S, Yasuhira S, Kanno K, Nakayama I, Sugiyama T, Wakabayasi G, Masuda T, Mori N
    • Journal Title

      Int.J.Oncol.

      Volume: 40(3) Pages: 695-702

    • DOI

      10.3892/ijo.2011.1291

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 多能性維持関連因子NACC1(nucleus accumbens-associated protein 1)の細胞周期特異的リン酸化の制御2012

    • Author(s)
      葛西秋宅、加茂政晴、柴崎晶彦、安平進士、前沢千早
    • Organizer
      代35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 肝細胞癌培養細胞株におけるHGF/METシグナルに与える細胞質内ヒストン脱アセチル化酸素 HDAC6発現の影響2012

    • Author(s)
      菅野公徳,前沢千早,柴崎晶彦,安平進士,葛西秋宅,石川雄一,及川浩樹,増田友之
    • Organizer
      第101回日本病理学会
    • Place of Presentation
      東京都新宿区
    • Related Report
      2012 Research-status Report
  • [Presentation] 悪性黒色腫における抗チューブリン剤耐性はBCL2とBCL-XLによって規定される2012

    • Author(s)
      渡辺彩乃,安平進士,葛西秋宅,柴崎晶彦,赤坂俊英,増田友之,前沢千早
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 多能性維持関連因子NACC1(nucleus accumbens-associated protein 1)の細胞周期特異的リン酸化の制御2012

    • Author(s)
      葛西秋宅,加茂政晴,柴崎晶彦,安平進士,前沢千早
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 悪性黒色腫細胞株の網羅的エクソーム解析による新規関連遺伝子の検索2012

    • Author(s)
      柴崎晶彦,三浦慎平,安平進士,葛西秋宅,赤坂俊英,増田友之,前沢千早
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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