| Project/Area Number |
24790398
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | National Center for Geriatrics and Gerontology |
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Principal Investigator |
KANEKO Keiko 独立行政法人国立長寿医療研究センター, 運動器疾患研究部, 研究員 (60532976)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 骨代謝 / 骨芽細胞 / 骨細胞 / インテグリン / 遺伝子改変マウス / シグナル伝達 / 細胞骨格 / シアストレス / ノックアウトマウス / YAP / ずり応力 |
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| Research Abstract |
Osteoblast lineage cells, especially osteocytes, are thought to be a primary mechanosensory cell in bone. However, the identity of the mechano-receptor and downstream mechno signaling pathways remain largely unknown. Using primary osteoblastic cells mechanically stimulated with fluid shear stress, an intracellular kinase cascade involving c-Src, p130Cas and JNK was identified the downstream of integrin alpha-v. Mice deficient in integrin alpha-v in the osteoblast lineage exhibited an impaired skeletal response to ulnar loading, in terms of Sost expression, pointing to the role of integrin alpha-v in mechano-sensing in vivo. Thus, integirn alpha-v may be integral to a mechanosensing machinery in osteoblast lineage cells and involved in the activation of a Src-JNK-YAP/TAZ pathway in response to mechanical stimulation.
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