Genome-wide mutation analysis of malaria mutator: epidemiological and experimental study
| Project/Area Number |
24790401
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
HONMA Hajime 東京女子医科大学, 医学部, 助教 (10617468)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | マラリア / ミューテーター / Plasmodium / 分子疫学 / 薬剤耐性 |
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| Outline of Final Research Achievements |
Detailed mutational trend of a mutator malaria parasite possessing proofreading deficient DNA polymerase δ was analyzed. The mutation spectrum was biased, and the base substitution of the parasite was influenced by sequence context. By using the mutator parasite, chloroquine resistant parasites were successfully generated. DNA sequencing analysis of genes involved in genome replication and/or stability with global Plasmodium falciparum DNA samples was also conducted, and fundamental information on the genetic polymorphism was obtained.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Generation of rodent malaria parasites with a high mutation rate by destructing proofreading activity of DNA polymerase δ2014
Author(s)
Honma H, Hirai M, Nakamura S, Hakimi H, Kawazu S, Nirianne MQP, Hisaeda H, Matsuoka H, Kawai S, Endo H, Yasunaga T, Ohashi J, Mita T, Horii T, Furusawa M, Tanabe K
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Journal Title
DNA Research
Volume: なし
Pages: 1-8
Related Report
Peer Reviewed
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