| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
Many bacterial pathogens manipulate cellular functions of host cells by injecting virulence proteins(T3SS effectors) into host cells using T3SS apparatus. Structural modeling analysis and biochemical experiments revealed that substrate selection and the recognition in T3SS secretion are conducted by the similar mechanism as used in the system of AAA+ protease ClpXP-substrate interaction. Based on the findings, structural property analysis was integrated into the effector prediction system, and the prediction accuracy was further improved. Experimental validation for 9 putative Salmonella effectors listed by this program revealed that five novel effectors including STM1239 were confirmed to secrete into supernatant of culture media.
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