Elucidation of structural mechanism of substrate selection and secretion signal in T3SS secretion by theoretical and experimental structural biology
Project/Area Number |
24790411
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including Mycology)
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Research Institution | Chiba University |
Principal Investigator |
SATO Yoshiharu 千葉大学, 薬学研究科(研究院), 助教 (00554586)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Keywords | サルモネラ / III型分泌装置 / エフェクター / 病原因子 / バイオインフォマティクス / III型分泌機構 / 国際情報交換 / カナダ |
Research Abstract |
Many bacterial pathogens manipulate cellular functions of host cells by injecting virulence proteins(T3SS effectors) into host cells using T3SS apparatus. Structural modeling analysis and biochemical experiments revealed that substrate selection and the recognition in T3SS secretion are conducted by the similar mechanism as used in the system of AAA+ protease ClpXP-substrate interaction. Based on the findings, structural property analysis was integrated into the effector prediction system, and the prediction accuracy was further improved. Experimental validation for 9 putative Salmonella effectors listed by this program revealed that five novel effectors including STM1239 were confirmed to secrete into supernatant of culture media.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] PCDq : human protein complex database with quality index which summarizes different levels of evidences of protein complexes predicted from H-Invitational protein-protein interactions integrative dataset2012
Author(s)
Kikugawa, S., Nishikata, K., Murakami, K., Sato, Y., Suzuki, M., Altaf-Ul-Amin, Md., Kanaya, S., Imanishi, T
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Journal Title
BMC systems biology
Volume: 6(Suppl 2)
Related Report
Peer Reviewed
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[Journal Article] Prediction of protein-destabilizing polymorphisms by manual curation with protein structure2012
Author(s)
Gough, CA., Homma, K., Yamaguchi-Kabata, Y., Shimada, MK., Chakraborty, R., Fujii, Y., Iwama, H., Minoshima, S., Sakamoto, S., Sato, Y. et al
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation2012
Author(s)
Hayashida, K., Hara, Y., Abe, T., Yamasaki, C., Toyoda, A., Kosuge, T., Suzuki, Y., Sato, Y. et al
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Journal Title
Related Report
Peer Reviewed
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