Xenophagy membrane dynamics

• Project/Area Number: 24790413
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NOZAWA Takashi 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (10598858)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: オートファジー / A群レンサ球菌 / Rabタンパク質 / Rab GTPase

Research Abstract

We could identify newly 4 Rab GTPases as an autophagy regulator during bacteial infection. These Rab proteins were not involved in starvation-induced autophagy, suggesting these regulators are responsible for autophagic membrane dynamics for bacteria.

Research Products

• [Journal Article] Development of two-step multiple PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis (2014)
  • Author(s): M. Okura, C. Lachance, M. Osaki, T. Sekizaki, F. Maruyamam, T. Nozawa, Nakagawa, S. Hamada, C. Rossignol, M. Gottschalk, D. Takamatsu
  • Journal Title: J. Clin. Microbiol Volume: 52 Pages: 1714-9
  • Peer Reviewed
• [Journal Article] Development of a two-step multiplex PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis (2014)
  • Author(s): Okura M, Lachance C, Osaki M, Sekizaki T, Maruyama F, Nozawa T, Nakagawa I, Hamada S, Rossignol C, Gottschalk M, Takamatsu D
  • Journal Title: J Clin Microbiol Volume: 52 Issue: 5 Pages: 1714-1719
  • DOI: 10.1128/jcm.03411-13
  • Peer Reviewed / Open Access
• [Journal Article] Endogenous Nitrated Nucleotide Is a Key Mediator of Autophagy and Innate Defense against Bacteria (2013)
  • Author(s): Ito C, Saito Y, Nozawa T, Fujii S, Sawa T, Inoue H, ... & Arimoto H
  • Journal Title: Mol Cell
  • Peer Reviewed
• [Journal Article] CRISPR regulation of intra-species diversification by limiting IS transposition and inter-cellular recombination (2013)
  • Author(s): T. Watanabe, T. Nozawa, C. Aikawa, A. Amano, F. Maruyama, and I. Nakagawa
  • Journal Title: Genome Biol. Evol Volume: 5 Pages: 1099-114
  • Peer Reviewed
• [Journal Article] Genetic Analysis of Capsular Polysaccharide Synthesis Gene Clusters from All Serotypes of Streptococcus suis : Potential Mechanisms for the Generation of Capsular Variation (2013)
  • Author(s): M. Okura, D. Takamatsu, F. Maruyama, T. Nozawa, I. Nakagawa, M. Osaki, T. Sekizaki, M. Gottschalk, Y. Kumagai, and S. Hamada
  • Journal Title: Appl. Environ. Microbiol Volume: 79 Pages: 2796-2806
  • Peer Reviewed
• [Journal Article] Endogenous nitrated nucleotide is a key mediator of autophagy and innate defense against bacteria. (2013)
  • Author(s): Ito C, Saito Y, Nozawa T, Fujii S, Sawa T, Inoue H, Matsunaga T, Khan S, Akashi S, Hashimoto R, Aikawa C, Takahashi E, Sagara H, Komatsu M, Tanaka K, Akaike T, Nakagawa I, Arimoto H.
  • Journal Title: Mol. Cell Volume: 52 Issue: 6 Pages: 794-804
  • DOI: 10.1016/j.molcel.2013.10.024
  • Peer Reviewed / Open Access
• [Journal Article] CRISPR regulation of intraspecies diversification by limiting IS transposition and intercellular recombination (2013)
  • Author(s): Watanabe T, Nozawa T, Aikawa C, Amano A, Maruyama F, Na kagawa I.
  • Journal Title: Genome Biology and Evolution Volume: 5 Issue: 6 Pages: 1099-1114
  • DOI: 10.1093/gbe/evt075
  • Peer Reviewed
• [Journal Article] Genetic analysis of capsular polysaccharide synthesis gene clusters from all serotypes of Streptococcus suis : potential mechanisms for generation of capsular variation (2013)
  • Author(s): Okura M, Takamatsu D, Maruyama F, Nozawa T, Nakagawa I, Osaki M, Sekizaki T, Gottschalk M, Kumagai Y, Hamada S
  • Journal Title: Appl Environ Microbiol Volume: 79 Issue: 8 Pages: 2796-2806
  • DOI: 10.1128/aem.03742-12
  • Peer Reviewed
• [Journal Article] The small GTPases Rab9A and Rab23 function at distinct steps in autophagy during Group A Streptococcus infection (2012)
  • Author(s): Nozawa T, Aikawa C, Goda A, Maruyama F, Hamada S, Nakagawa I
  • Journal Title: Cell Microbiol Volume: 14 Pages: 1149-65
  • Peer Reviewed
• [Journal Article] The small GTPases Rab9A and Rab23 function at distinct steps in autophagy during Group A Streptococcus infection (2012)
  • Author(s): Nozawa T, Aikawa C, Goda A, Maruyama F, Hamada S, Nakagawa I
  • Journal Title: Cellular Microbiology Volume: 14 Pages: 1149-1165
  • Peer Reviewed
• [Presentation] Atg5-independent/Rab9a-dependent target of intracellular group A streptococcus (2014)
  • Author(s): 野澤孝志,野澤敦子,相川知宏,丸山史人,中川一路
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀(東京)
• [Presentation] Contribution of recycling endosome in Group A Streptococcus induced autophagosome formation (2014)
  • Author(s): Bijaya Haobam,野澤孝志,野澤敦子,尾田誠一郎,相川知宏,丸山史人,中川一路
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀(東京)
• [Presentation] The Small GTPase Rab30 regulates anti-bacterial autophagy (2014)
  • Author(s): 尾田誠一郎,野澤孝志,野澤敦子,相川知宏,丸山史人,中川一路
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀(東京)
• [Presentation] Atg5-independent/Rab9a-dependent target of intracellular group A streptococcus" (2014)
  • Author(s): 野澤孝志, 野澤敦子, 相川知宏, 丸山史人, 中川一路
  • Organizer: 第87回 日本細菌学会総会
  • Place of Presentation: タワーホール船堀（東京）
• [Presentation] A群レンサ球菌によるオートファジーを制御するRabタンパク質群 (2013)
  • Author(s): 野澤孝志、相川知宏、郷田瑛、丸山史人、中川一路
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉県
  • Year and Date: 2013-03-18
• [Presentation] Atg5-independent/Rab9A-dependent target of intracellular Group A Streptococcus (2013)
  • Author(s): Takashi Nozawa, Chihiro Aikawa, Shinichiro Oda, Akira Goda, Fumito Maruyama, and Ichiro Nakagawa
  • Organizer: FEMS 2013 (5th congress of european microbiologists)
  • Place of Presentation: ドイツ
• [Presentation] A群レンサ球菌によるオートファジーを制御するRabタンパク質群 (2013)
  • Author(s): 野澤孝志、相川知宏、郷田瑛、丸山史人、中川一路
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ国際会議場（千葉県）
  • Invited
• [Presentation] Atg5-independent/Rab9A-dependent target of intracellular Group A Streptococcus (2013)
  • Author(s): Takashi Nozawa, Chihiro Aikawa, Shinichiro Oda, Akira Goda, Fumito Maruyama, and Ichiro Nakagawa
  • Organizer: FEMS 2013 (5th congress of european microbiologists)
  • Place of Presentation: ドイツ・ライプチヒ
• [Presentation] Unique distribution of emm types and superantigen gene profiles of Group A Streptococcus isolates in Thailand (2013)
  • Author(s): Takashi Nozawa, Wantana Paveenkittiporn, Surang Dejsirilert, Shigeyuki Hamada
  • Organizer: AARF2013 (新興・再興感染症に関するアジア・アフリカリサーチフォーラム)
  • Place of Presentation: 東京医科歯科大学 (東京都)
• [Presentation] The small GTPases Rab9A and Rab23 function at distinct steps in autophagy during Group A Streptococcus infection (2012)
  • Author(s): Takashi Nozawa, Chihiro Aikawa, Akira Goda, Fumito Maruyama, Ichiro Nakagawa
  • Organizer: 第11回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 兵庫県
  • Year and Date: 2012-09-14
• [Presentation] バクテリオファージによる宿主細菌への獲得免疫の付与 (2012)
  • Author(s): 野澤孝志
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎県
  • Year and Date: 2012-03-28
• [Presentation] Rab タンパク質による A 群レンサ球菌感染誘導オートファジーの制御機構 (2012)
  • Author(s): 野澤孝志、 相川知宏、郷田瑛、丸山史人、中川一路
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 奥羽大学(福島県)
• [Presentation] ファージとその防御機構に着目したA群レンサ球菌ゲノムの種内多様性 (2012)
  • Author(s): 野澤孝志、細見晋吾、相川知宏、丸山史人、中川一路
  • Organizer: 第2回NGS現場の回研究会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪府)
• [Presentation] The small GTPases Rab9A and Rab23 function at distinct steps in autophagy during Group A Streptococcus infection (2012)
  • Author(s): Takashi Nozawa, Chihiro Aikawa, Akira Goda, Fumito Maruyama, Ichiro Nakagawa
  • Organizer: 第11回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場 (兵庫県)
• [Book] Rab Proteins in Autophagy: Streptococcus Model. Chapter contribution for the book "Autophagy, Infection and the Immune Response" (2014)
  • Author(s): Nozawa T and Nakagawa I
  • Publisher: Wiley-Blackwell
• [Remarks]
  • URL: http://www.tmd.ac.jp/grad/bac/index.html
• [Remarks] 所属研究室ホームページ
  • URL: http://www.tmd.ac.jp/grad/bac/index.html
• [Remarks] 東京医科歯科大学 大学院医歯学総合研究科 細菌感染制御学分野
  • URL: http://www.tmd.ac.jp/grad/bac/index.html