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Characterization of proteases produced by virulent strain of Aeromonas

Research Project

Project/Area Number 24790417
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including Mycology)
Research InstitutionOkayama University

Principal Investigator

TAKAHASHI Eizo  岡山大学, 医歯(薬)学総合研究科, 助教 (70379733)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywordsアエロモナス (エロモナス) / メタロプロテアーゼ / セリンプロテアーゼ / エラスチン / スキムミルク / Aeromonas
Research Abstract

The properties of metalloprotease produced by A. hydrophila and of serine protease produced by A. trota were examined. A. hydrophila produces the metalloprotease as the intermediate form, which is composed of mature domain and carboxy-terminal domain, into milieu. The intermediate form released into milieu is immediately cleaved to remove the carboxy-terminal domain and turns into mature form. To clear the property of the intermediate form, we constructed the plasmid harboring metalloprotease gene and introduced it into Escherichia coli. The examination of the metalloprotease produced by the transformed cell showed that the intermediate form is not detected, though the mature form is detected.
Subsequently, I showed that A. trota produced much amount of serine protease at 25 degree than at 37 degree and that the addition of skim milk in the medium promoted the production of serine protease by A. trota.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (35 results)

All 2014 2013 2012

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (18 results) Book (5 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Properties of hemolysin and protease produced by Aeromonas trota2014

    • Author(s)
      Eizo Takahashi, Haruka Ozaki, Yoshio Fujii, Hidetomo Kobayashi, Hiroyasu Yamanaka, Sakae Arimoto, Tomoe Negishi, Keinosuke Okamoto
    • Journal Title

      PLOS ONE

      Volume: 9 (3) Issue: 3 Pages: e91149-e91149

    • DOI

      10.1371/journal.pone.0091149

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Oral Administration of Non-genotoxic Hepato-hypertrophic Compounds on Metabolic Potency of Rat Liver2014

    • Author(s)
      Xing Fang, Tatsuo Nunoshiba, Midori Yoshida, Akiyoshi Nishikawa, Kiyomitsu Nemoto, Masakuni Degawa, Sakae Arimoto, Keinosuke Okamoto, Eizo Takahashi, Tomoe Negishi
    • Journal Title

      Genes and Environment

      Volume: 36 (1) Pages: 1-9

    • NAID

      130004481209

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spermidine-analogous triamines suppressed the growth of Candida albicans2013

    • Author(s)
      Eizo Takahashi, Seiji Oono et al.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 36(9) Pages: 1440-1447

    • NAID

      130003361514

    • URL

      https://www.jstage.jst.go.jp/article/bpb/36/9/36_b13-00148/_pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of carboxy terminal domain of metalloprotease of elastolytic Aeromonas hydrophila2013

    • Author(s)
      Eizo Takahashi, Hidetomo Kobayashi et al.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 36(7) Pages: 1174-1182

    • NAID

      130003361477

    • URL

      https://www.jstage.jst.go.jp/article/bpb/36/7/36_b13-00161/_pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] エロモナス感染症2013

    • Author(s)
      髙橋栄造
    • Journal Title

      化学療法の領域

      Volume: 29(7) Pages: 1478-1481

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of carboxy terminal domain of metalloprotease of elastolytic Aeromonas hydrophila2013

    • Author(s)
      Eizo Takahashi, Hidetomo Kobayashi, Hiroyasu Yamanaka, Mayu Nakanishi, Arisa Tateishi, Takemi Abe, Sakae Arimoto, Tomoe Negishi, Keinosuke Okamoto
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 36 (7) Pages: 1174-1182

    • NAID

      130003361477

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spermidine-analogous triamines suppressed the growth of Candida albicans.2013

    • Author(s)
      Eizo Takahashi, Seiji Oono, Shigeo Yamamoto, Sakae Arimoto, Tomoe Negishi, Keinosuke Okamoto
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 36 (9) Pages: 1440-1447

    • NAID

      130003361514

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] エロモナス感染症2013

    • Author(s)
      高橋栄造
    • Journal Title

      化学療法の領域

      Volume: 29 (7) Pages: 1478-1481

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Distinct pathways for repairing mutagenic lesions induced by methylating and ethylating agents2013

    • Author(s)
      Taira, K., Kaneto, S., Nakano, K., Satanabe, S., Takahashi, E., Arimoto S., Okamoto, K., Schaaper, R.M., Negishi, K., Negishi, T.
    • Journal Title

      Mutagenesis

      Volume: 28 Issue: 3 Pages: 341-350

    • DOI

      10.1093/mutage/get010

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Production and properties of lipase of Aeromonas sobria2012

    • Author(s)
      Eizo Takahashi, Hidenobu Ito et al.
    • Journal Title

      Microbiology and Immunology

      Volume: 56(5) Issue: 5 Pages: 295-307

    • DOI

      10.1111/j.1348-0421.2012.00445.x

    • NAID

      40019309951

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Skim milk promotes the production of extracellular serine protease by Aeromonas spp2014

    • Author(s)
      Eizo Takahashi
    • Organizer
      第86回日本細菌学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Production and characterization of serine protease produced by Aeromonas trota2014

    • Author(s)
      Eizo Takahashi
    • Organizer
      The 48th Annual joint meeting on cholera and other bacterial enteric infections
    • Place of Presentation
      Dhaka, Bangladesh
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Inductive effect of skim milk on the production of extracellular serine protease by Aeromonas spp2014

    • Author(s)
      Eizo Takahashi
    • Organizer
      Asian-African Research Forum 2014
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] Inductive effect of skim milk on the production of extracellular serine protease by Aeromonas spp.2014

    • Author(s)
      Eizo Takahashi, Thandavarayan Ramamurthy, Sumio Shinoda, Keinosuke Okamoto
    • Organizer
      Asian-African Research Forum 2014
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Skim milk promotes the production of extracellular serine protease by Aeromonas spp.2014

    • Author(s)
      Eizo Takahashi, Keinosuke Okamoto
    • Organizer
      第86回 日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] アエロモナスの産生するセリンプロテアーゼのスキムミルクによる発現誘導2013

    • Author(s)
      高橋栄造、岡本敬の介
    • Organizer
      第66回 日本細菌学会中国・四国支部総会
    • Place of Presentation
      呉
    • Related Report
      2013 Annual Research Report
  • [Presentation] Aeromonas sobria セリンプロテアーゼのウシ胎児血清による産生抑制2013

    • Author(s)
      延廣昌典、高橋栄造、岡本敬の介
    • Organizer
      第66回 日本細菌学会中国・四国支部総会
    • Place of Presentation
      呉
    • Related Report
      2013 Annual Research Report
  • [Presentation] βポア形成毒に対する阻害剤の探索2013

    • Author(s)
      藤波千明、高橋栄造、竹内靖雄、岡本敬の介
    • Organizer
      第52回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
  • [Presentation] アエロモナスの産生するメタロプロテアーゼ中間体の精製方法の検討2013

    • Author(s)
      丸尾陽成、高橋栄造、岡本敬の介
    • Organizer
      第52回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Aeromonas trota セリンプロテアーゼのスキムミルクによる産生誘導について2013

    • Author(s)
      山下哲也、高橋栄造、尾崎晴香、岡本敬の介
    • Organizer
      第52回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
  • [Presentation] アエロモナスの菌体外セリンプロテアーゼのスキムミルクによる産生亢進2013

    • Author(s)
      高橋栄造、山下哲也、尾崎晴香、岡本敬の介
    • Organizer
      第47回 腸炎ビブリオシンポジウム
    • Place of Presentation
      東広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characterization of exotoxins produced by Aeromonas trota2013

    • Author(s)
      Eizo Takahashi, Thandavarayan Ramamurthy, Yoshifumi Takeda, Sumio Shinoda, Keinosuke Okamoto
    • Organizer
      Asian-African Research Forum
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] The production of serine protease of Aeromonas trota is induced by skim milk.2013

    • Author(s)
      髙橋栄造、岡本敬の介
    • Organizer
      第86回 日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
  • [Presentation] スペルミジン誘導体トリアミンのCandida albicansに対する抗真菌活性2013

    • Author(s)
      髙橋栄造、大野成司、山本重雄、岡本敬の介
    • Organizer
      日本薬学会 第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Aeromonas trotaセリンプロテアーゼの性状解析2012

    • Author(s)
      髙橋栄造、尾崎晴香、山下哲也、岡本敬の介
    • Organizer
      第59回トキシンシンポジウム
    • Place of Presentation
      帯広
    • Related Report
      2012 Research-status Report
  • [Presentation] Salt in surroundings influences the production of exotoxins by Aeromonas sobria2012

    • Author(s)
      Eizo Takahashi, Keinosuke Okamoto
    • Organizer
      Bioactive Okayama 2012
    • Place of Presentation
      岡山
    • Related Report
      2012 Research-status Report
  • [Presentation] 培養温度により発現調節されるAeromonas trotaのセリンプロテアーゼについて2012

    • Author(s)
      髙橋栄造、岡本敬の介
    • Organizer
      第65回 日本細菌学会中国・四国支部総会
    • Place of Presentation
      徳島
    • Related Report
      2012 Research-status Report
  • [Presentation] 培養温度により産生調節されるAeromonas trotaのセリンプロテアーゼの解析2012

    • Author(s)
      尾崎晴香、髙橋栄造、山下哲也、岡本敬の介
    • Organizer
      第51回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      松江
    • Related Report
      2012 Research-status Report
  • [Book] Handbook of Proteolytic Enzymes (Third Ed), Chapter 118 PA peptidase (Aeromonas)2013

    • Author(s)
      Eizo Takahashi, Keinosuke Okamoto
    • Publisher
      Elsevier
    • Related Report
      2013 Final Research Report
  • [Book] Handbook of Proteolytic Enzymes (Third Ed), Chapter 119 Vibriosin2013

    • Author(s)
      Shin-ichi Miyoshi, Keinosuke Okamoto, Eizo Takahashi
    • Publisher
      Elsevier
    • Related Report
      2013 Final Research Report
  • [Book] Handbook of Proteolytic Enzymes (Third Ed), Chapter 706 ASP peptidase2013

    • Author(s)
      Eizo Takahashi, Keinosuke Okamoto
    • Publisher
      Elsevier
    • Related Report
      2013 Final Research Report
  • [Book] Handbook of Proteolytic Enzymes (Third Ed)(Eds, Neil D. Rawlings and Guy S. Salvesen)2013

    • Author(s)
      Eizo Takahashi, Keinosuke Okamoto
    • Publisher
      Elsevier
    • Related Report
      2013 Annual Research Report
  • [Book] Handbook of Proteolytic Enzymes (Third Ed)(Eds, Neil D. Rawlings and Guy S. Salvesen)2013

    • Author(s)
      Shin-ich Miyoshi, Keinosuke Okamoto, Eizo Takahashi
    • Publisher
      Elsevier
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] インドロキノリン誘導体を含む溶血毒阻害用組成物2013

    • Inventor(s)
      髙橋栄造、岡本敬の介、竹内靖雄、黒田照夫
    • Industrial Property Rights Holder
      岡山大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-163052
    • Filing Date
      2013-08-06
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] インドロキノリン誘導体を含む溶血毒阻害用組成物2013

    • Inventor(s)
      髙橋栄造, 岡本敬の介, 竹内靖雄, 黒田照夫
    • Industrial Property Rights Holder
      岡山大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-163052
    • Filing Date
      2013-08-06
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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