| Project/Area Number |
24790419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | サルモネラ / γーグルタミルトランスペプチダーゼ |
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| Research Abstract |
To understand the role of gamma-glutamyltranspeptidase (GGT) in Salmonella, I investigated whether GGT is contributed to the Salmonella virulence. Although I analyzed the functions of GGT using the ggt gene-disrupted mutant, I could not find the association with GGT and Salmonella virulence.
Salmonella was cultured at 20 C, GGT activity was elevated than that at 37 C. When I investigated the molecular mechanisms on GGT activity, I found that GGT activity in the hfq gene-disrupted mutant (hfq-mutant) was decreased compared with that of wild-type Salmonella. In addition, GGT activity in the hfq gene-complemented hfq-mutant was elevated compared with that of hfq-mutant. These results suggested that the RNA chaperone Hfq is involved in the GGT activity. Therefore, I speculated that production of GGT in Salmonella is the low temperature dependent manner and a novel non-coding small RNA in Salmonella should be associated with the exhibition of GGT activity.
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