Ubiquitination mechanism of intracellular bacteria

• Project/Area Number: 24790420
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Shizuoka
• Principal Investigator: YOSHIKAWA YUKO 静岡県立大学, 食品栄養科学部, 助教 (00580523)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細菌 / リステリア / 感染症 / オートファジー / ユビキチン

Outline of Final Research Achievements

Poly-ubiquitination is a trigger of selective autophagy for clearance of injured mitochondria and aggregates derived from denatured proteins. Some of intracellular bacteria are also ubiquitinated and then eliminated by selective autophagy in the same manner. In this study, we attempted to identify the ubiquitination mechanisms of ActA-deficient Listeria monocytogenes (delta actA). A total of 29 candidates (2 listerial and 27 host proteins) were identified by LC-MS/MS analysis. E3 is known to be playing an important role for ubiquitination of other intracellular bacteria, such as Salmonella and Mycobacterium tuberculosis. There were 3 kinds of ubiquitin ligases (E3) in the host candidates. We therefore analyzed the localization of E3s in the cells infected with delta actA. As a result, E3-1 was accumulated around the bacterial cells 1 hour postinfection simultaneously with starting point of the delta actA ubiquitination.

Research Products

• [Journal Article] Simple and sensitive analysis of histamine and tyramine in Japanese soy sauces and their intermediates using the stable isotope dilution HILIC–MS/MS method. (2014)
  • Author(s): Todoroki, K., Ishii, Y., Miyauchi, C., Kitagawa, S., Min, J. Z., Inoue, K., Yamanaka, T., Suzuki, K., Yoshikawa, Y., Ohashi, N. and Toyo'Oka, T.
  • Journal Title: Journal of Agricultual Food Chemistry Volume: 62 Issue: 26 Pages: 6206-6211
  • DOI: 10.1021/jf500767p
  • Peer Reviewed
• [Journal Article] Anaplasma phagocytophilum antibodies in humans, Japan, 2010-2011. (2014)
  • Author(s): Gaowa, Yoshikawa, Y., Ohashi, N., Wu, D., Kawamori, F., Ikegaya, A., Watanabe, T., Saitoh, K., Takechi, D., Murakami, Y., Shichi, D., Aso, K., and Ando, S.
  • Journal Title: Emerging Infectious Diseases Volume: 20 Issue: 3 Pages: 508-509
  • DOI: 10.3201/eid2003.131337
  • Peer Reviewed / Open Access
• [Journal Article] Analysing the substrate multispecificity of a proton-coupled oligopeptide transporter using a dipeptide library (2013)
  • Author(s): Keisuke Ito*, Aya Hikida, Vu Thi Tuyet Lan, Takayasu Motoyama, Sayuri Kitagawa, Yuko Yoshikawa, Ryuji Kato, Yasuaki Kawarasaki
  • Journal Title: Nat. Commun. Volume: 4 Issue: 1 Pages: 2502-2502
  • DOI: 10.1038/ncomms3502
  • Peer Reviewed
• [Journal Article] Application of a simple method using minute particles of amorphous calcium phosphate for recovery of norovirus from cabbage, lettuce, and ham. (2013)
  • Author(s): Shinohara M. Uchida K., Shimada S., Tomioka K., Suzuki N., Minegishi T., Kawahashi S., Yoshikawa Y., and Ohashi N.
  • Journal Title: Journal of Virological Methods Volume: 187 Issue: 1 Pages: 153-158
  • DOI: 10.1016/j.jviromet.2012.09.025
  • Peer Reviewed
• [Journal Article] Human granulocytic anaplasmosis in Japan. (2013)
  • Author(s): Ohashi, N., Gaowa, Wuritu, Kawamori, F., Wu, D., Yoshikawa, Y., Chiya, S., Fukunaga, K., Funato, T., Shiojiri, M., Nakajima, H., Hamauzu, Y., Takano, A., Kawabata, H., Ando, S., and Kishimoto, T
  • Journal Title: Emerging Infectious Diseases Volume: 19 Issue: 2 Pages: 289-292
  • DOI: 10.3201/eid1902.120855
  • Peer Reviewed
• [Journal Article] Rickettsiae in ticks, 2007-2011 Japan (2013)
  • Author(s): Gaowa, Ohashi, N., Aochi, M., Wuritu, Wu, D., Yoshikawa, Y., Kawamori, F., Honda, T., Fujita, H., Takada, N., Oikawa, Y., Kawabata, H., Ando, S., and Kishimoto, T.
  • Journal Title: Emerging Infectious Diseases Volume: 19 Issue: 2 Pages: 338-340
  • DOI: 10.3201/eid1902.120856
  • Peer Reviewed
• [Journal Article] Guideline for the use and interpretation of assays for monitoring autophagy (2012)
  • Author(s): Klionsky DJ,et al
  • Journal Title: Autophagy Volume: 8 Issue: 4 Pages: 445-544
  • DOI: 10.4161/auto.19496
  • Peer Reviewed
• [Presentation] 静岡県の野生動物から得たマダニ類 (Ixodidae) (2015)
  • Author(s): 高田 歩, 呉 東興, 吉川 悠子, 大橋 典男
  • Organizer: 第67回日本衛生動物学会大会
  • Place of Presentation: 金沢
  • Year and Date: 2015-03-28
• [Presentation] 漬物より分離した乳酸菌が有する抱合胆汁酸脱抱合酵素の解析 (2015)
  • Author(s): 岩井克機, 尾崎順哉, 吉川悠子, 大橋典男
  • Organizer: 日本農芸化学会2015年度岡山大会
  • Place of Presentation: 岡山
  • Year and Date: 2015-03-28
• [Presentation] グラム陰性菌リポ多糖構成分子lipid AのLC-MS分析法の確立 (2015)
  • Author(s): 大石美月、吉川悠子、大島寛史、三好規之
  • Organizer: 日本農芸化学会2015年度岡山大会
  • Place of Presentation: 岡山
  • Year and Date: 2015-03-28
• [Presentation] アナプラズマ症の特異抗体検出による患者探索の現状報告 (2014)
  • Author(s): 呉東興，高娃，吉川悠子，川森文彦，池ヶ谷朝香，川上万里，岸本壽男，森田裕司，中堂園文子，御供田睦代，能勢裕久，池田賢一，増澤俊幸，安藤秀二，大橋典男
  • Organizer: 第21回リケッチア研究会 研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2014-12-21
• [Presentation] ラーゲン誘発性関節炎モデルマウスにおけるトマトサポニンの抗炎症効果 (2014)
  • Author(s): 吉川悠子，片柳悠紀，三好規之，福富竜太，大橋典男
  • Organizer: 第19回日本フードファクター学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2014-11-08
• [Presentation] Anaplasma phagocytophilum感染患者血清中に存在する抗体の検出法に関する検討 (2014)
  • Author(s): 呉東興，高娃，吉川悠子，川森文彦，川上万理，岸本壽男，森田裕司，増澤俊幸，安藤秀二，大橋典男
  • Organizer: 第97回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-31
• [Presentation] 南アルプス産トガリネズミ2種に寄生するケモチダニ類の新たな知見 (2014)
  • Author(s): 高田歩，佐々木彰央，吉川悠子，大橋典男
  • Organizer: 2014年度日本ダニ学会
  • Place of Presentation: 盛岡
  • Year and Date: 2014-10-18
• [Presentation] Tick-associated Anaplasmataceae pathogens in Japan. (2014)
  • Author(s): Ohashi N., Yoshikawa Y., Gaowa, Wuritu, Wu D., Kawamori F.
  • Organizer: XIV International Congress of Acarology
  • Place of Presentation: 京都
  • Year and Date: 2014-07-18
• [Presentation] Anaplasma phagocytophilumの細胞内感染機構 (2014)
  • Author(s): Yoshikawa Y., Sugimoto K., Gaowa, Ohashi N.
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] Protective ability of lactic acid bacteria isolated from wasabi-zuke for Citrobacter rodentium infection in mouse. (2013)
  • Author(s): Yamazaki M., Yoshikawa Y., Sugimoto K., Serizawa T., Tanaka M., Murakami T., Masuda R., Kobayashi Y., Koide H., Oku N., and Ohashi N.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 腸管病原性細菌の感染防御効果を有する乳酸菌の探索のためのin vivoイメージング (2012)
  • Author(s): 山崎芽生、吉川悠子、奥直人、大橋典男
  • Organizer: 日本乳酸菌学会2012年度大会
  • Place of Presentation: 筑波
• [Presentation] Overview of tick-associated Rickettsiales bacteria in Japan. (2012)
  • Author(s): Ohashi N., Yoshikawa Y., and Masuzawa T.
  • Organizer: Russian Scientific Conference with International Participation “BASIC AND APPLIED ASPECTS OF INFECTIOUS PATHOLOGY”
  • Place of Presentation: Irkutsk, Russia
• [Presentation] 近年のアナプラズマを中心としたリケッチア関連細菌群の調査概要 (2012)
  • Author(s): 大橋典男、高娃、呉東興、烏日図、吉川悠子、川森文彦
  • Organizer: 第5回日本リケッチア症臨床研究会・第19回リケッチア研究会合同研究発表会
  • Place of Presentation: 大津
• [Presentation] Complete sequences and comparative analysis of IncN plasmids encoding blaCTX-M-2 from broiler and human origins (2012)
  • Author(s): 渕田琢、廣井みどり、川森文彦、吉川悠子、大橋典男
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Book] 改訂新版 健康と食の安全を考えた食品衛生学実験 (2013)
  • Author(s): 増田修一編著
  • Total Pages: 13
  • Publisher: アイ・ケイ・コーポレーション