Isolation and identification of TREM-1 binding proteins as candidate for TREM-1 ligands in neutrophil-like differentiated HL-60 cells
| Project/Area Number |
24790424
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | TREM-1 / Ligand / 好中球 / マクロファージ / 好中球NETs / ligand / neutrophils / NETs / GST-pull down / リガンド / 敗血症 / 血小板 |
|---|
| Outline of Final Research Achievements |
TREM-1 (triggering receptor expressed on myeloid cells-1) plays a role in inflammation by augmenting inflammatory responses via the production of proinflammatory cytokines in macrophage. Although natural TREM-1 ligands have not been identified, flow cytometrical analyses of the binding of labeled-recombinant TREM-1 suggest that TREM-1 ligands are expressed on neutrophils. Recent studies have demonstrated that neutrophil extracellular traps (NETs) was released from neutrophil to activate macrophage. Here we attempted to identify a potential TREM-1 ligand in the components of NETs.
HEK293 cells lysate of over-expressing TREM-1-GST fusion protein and nuclear extract from the neutrophil-like differentiated HL-60 cells were subjected to GST-pull down assay. As a result, about 72 kDa molecules were specifically bind to TREM-1. At present, these molecules were identified by mass spectrometric analysis, and measured ligand potency.
|
Report
(4 results)
Research Products
(8 results)
