| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
TREM-1 (triggering receptor expressed on myeloid cells-1) plays a role in inflammation by augmenting inflammatory responses via the production of proinflammatory cytokines in macrophage. Although natural TREM-1 ligands have not been identified, flow cytometrical analyses of the binding of labeled-recombinant TREM-1 suggest that TREM-1 ligands are expressed on neutrophils. Recent studies have demonstrated that neutrophil extracellular traps (NETs) was released from neutrophil to activate macrophage. Here we attempted to identify a potential TREM-1 ligand in the components of NETs.
HEK293 cells lysate of over-expressing TREM-1-GST fusion protein and nuclear extract from the neutrophil-like differentiated HL-60 cells were subjected to GST-pull down assay. As a result, about 72 kDa molecules were specifically bind to TREM-1. At present, these molecules were identified by mass spectrometric analysis, and measured ligand potency.
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