Roles of type 5 secretion system-derived proteins in the pathogenicity of Pseudomonas aeruginosa

• Project/Area Number: 24790426
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kurume University
• Principal Investigator: KIDA YUTAKA 久留米大学, 医学部, 講師 (30309752)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 緑膿菌 / プロテアーゼ / 感染 / ５型分泌 / 5型分泌

Outline of Final Research Achievements

Pseudomonas aeruginosa, an opportunistic pathogen, is a major cause of morbidity and mortality in immunocompromised hosts. P. aeruginosa possesses an arsenal of both cell-associated (flagella, pili, alginate/biofilm, etc.) and secreted (exotoxin A, proteases, type 3 secretion system effectors, etc.) virulence factors. However, little has been known about the type 5 secretion system (T5SS)-derived proteins involving in the pathogenicity of this organism. In this study, we tried to elucidate whether EprS, an autotransporter protein, secreted via T5SS functions as a virulence factor of P. aeruginosa. Although EprS is predicted to encode a serine protease, there is no published experimental evidence to confirm this. Then, we performed functional analyses of recombinant EprS secreted by Escherichia coli. Our results indicated that EprS, a serine protease, exhibits the substrate specificity, cleaving at the carboxyl side of basic residues and induces host inflammatory responses through PARs.

Research Products

• [Journal Article] Cytadherence of Mycoplasma pneumoniae induces inflammatory responses through autophagy and toll-like receptor 4. (2014)
  • Author(s): Shimizu T, Kimura Y, Kida Y, Kuwano K, Tachibana M, Hashino M, Watarai M.
  • Journal Title: Infection and Immunity Volume: vol. 82 Issue: 7 Pages: 3076-3086
  • DOI: 10.1128/iai.01961-14
  • Peer Reviewed / Open Access
• [Journal Article] The relationship between biofilm formations and capsule in Haemophilus influenzae (2014)
  • Author(s): Qin, L., Kida, Y., Ishiwada, N., Ohkusu, K., Kaji, C., Sakai, Y., Watanabe, K., Furumoto, A., Ichinose, A., Watanabe, H.
  • Journal Title: Journal of Infection and Chemotherapy Volume: 20 Issue: 3 Pages: 151-156
  • DOI: 10.1016/j.jiac.2013.06.001
  • Peer Reviewed
• [Journal Article] Protein phosphatase 2A dephosphorylates phosphoserines in nucleocytoplasmic shuttling and secretion of high mobility group box 1 (2013)
  • Author(s): Taira, J., Kida, Y., Kuwano, K., Higashimoto, Y.
  • Journal Title: J. Biochem. Volume: 154 Issue: 3 Pages: 299-308
  • DOI: 10.1093/jb/mvt056
  • Peer Reviewed
• [Journal Article] Roles of Pseudomonas aeruginosa-derived proteases as a virulence factor (2013)
  • Author(s): Kida, Y.
  • Journal Title: Nihon Saikingaku Zasshi Volume: 68 Pages: 313-323
  • NAID: 130003384698
  • Peer Reviewed
• [Journal Article] Impaired capsular polysaccharide is relevant to enhanced biofilm formation and lower virulence in Streptococcus pneumoniae. (2013)
  • Author(s): Qin L, Kida Y, Imamura Y, Kuwano K and Watanabe H.
  • Journal Title: J Infect Chemother Volume: 19 Issue: 2 Pages: 261-271
  • DOI: 10.1007/s10156-012-0495-3
  • NAID: 10031167893
  • Peer Reviewed
• [Journal Article] EprS, an autotransporter protein of Pseudomonas aeruginosa, possessing serine protease activity induces inflammatory responses through protease-activated receptors. (2013)
  • Author(s): Kida Y, Taira J, Yamamoto T, Higashimoto Y, Kuwano K.
  • Journal Title: Cell Microbiol. Volume: 15 Issue: 7 Pages: 1168-1181
  • DOI: 10.1111/cmi.12106
  • Peer Reviewed
• [Presentation] 病原性因子としての緑膿菌プロテアーゼの役割 (2015)
  • Author(s): 木田 豊
  • Organizer: 日本薬学会第135年会、一般シンポジウム
  • Place of Presentation: 兵庫県神戸市、神戸学院大学
  • Year and Date: 2015-03-28
• [Presentation] 緑膿菌の病原性因子としてのEprSオートトランスポータープロテアーゼの役割 (2015)
  • Author(s): 木田 豊、山本 武司、桑野 剛一
  • Organizer: 第88回日本細菌学会総会、一般演題
  • Place of Presentation: 岐阜県岐阜市、長良川国際会議場
  • Year and Date: 2015-03-26
• [Presentation] Role of bacterial proteases in pathogenesis (2014)
  • Author(s): Yutaka Kida
  • Organizer: 12th Japan-Korea International Symposium on Microbiology
  • Place of Presentation: Tokyo
  • Invited
• [Presentation] 緑膿菌オートトランスポータープロテアーゼEprSの機能解析 (2013)
  • Author(s): 木田 豊、山本武司、桑野剛一
  • Organizer: 第66回日本細菌学会九州支部総会
  • Place of Presentation: 長崎
• [Presentation] 緑膿菌オートトランスポータープロテアーゼEprSの機能解析 (2013)
  • Author(s): 木田 豊、山本 武司、桑野 剛一
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] 緑膿菌オートトランスポータープロテアーゼEprSの機能解析 (2012)
  • Author(s): 木田 豊、山本 武司、桑野 剛一
  • Organizer: 第65回日本細菌学会九州支部総会
  • Place of Presentation: 沖縄