| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Pseudomonas aeruginosa, an opportunistic pathogen, is a major cause of morbidity and mortality in immunocompromised hosts. P. aeruginosa possesses an arsenal of both cell-associated (flagella, pili, alginate/biofilm, etc.) and secreted (exotoxin A, proteases, type 3 secretion system effectors, etc.) virulence factors. However, little has been known about the type 5 secretion system (T5SS)-derived proteins involving in the pathogenicity of this organism. In this study, we tried to elucidate whether EprS, an autotransporter protein, secreted via T5SS functions as a virulence factor of P. aeruginosa. Although EprS is predicted to encode a serine protease, there is no published experimental evidence to confirm this. Then, we performed functional analyses of recombinant EprS secreted by Escherichia coli. Our results indicated that EprS, a serine protease, exhibits the substrate specificity, cleaving at the carboxyl side of basic residues and induces host inflammatory responses through PARs.
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