| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
In human intestines, there are many unknown, hardly cultivable and previously uncultivated bacteria. The aim of this study was to cultivate unknown and/or hardly cultivable bacteria in conditions close to the environment of original habitat, followed by to analyze the characteristics of those bacterial genome. This study showed that the bacteria in human gut were able to grow in medium made from human feces. Moreover, the bacteria of different types were detected by the fecal mediums which were sterilized with different methods. The 16S rRNA gene metagenomic analysis revealed that some of DNA sequences, which were isolated from bacteria grown in fecal mediums, did not show high identity to known bacterial rRNA genes in existing databases. These data suggested that unknown and/or hardly cultivable bacteria of human gut might be cultured from human fecal mediums.
|
