| Project/Area Number |
24790431
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
SHOUJI Yamamoto 国立感染症研究所, 細菌第一部, 主任研究官 (80469957)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | コレラ菌 / キチン / 形質転換 / シグナル伝達 / 二成分制御系 / センサーキナーゼ / 膜貫通型転写因子 / small RNA / non-coding RNA / 該当なし |
|---|
| Research Abstract |
In Vibrio cholerae, chitin, a polymer of GlcNAc, induces natural competence for genetic transformation. This study was done to understand the role of a novel transmembrane DNA-binding protein, named 'TfoS', in chitin-induced competence. The results suggested that: 1) TfoS acts as a transcriptional activator of the tfoR gene, encoding a chitin-induced small RNA essential for competence gene expression, 2) TfoS has no two-component system (TCS) signature domains, but its activity is strictly dependent on the chitin-responsive TCS sensor kinase ChiS, and 3) ChiS regulates the activity of TfoS using mechanisms distinct from classical phosphorelay reactions.
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