Development of a rapid and sensitive cell-fusion-based phenotypic HIV-1 tropism assay

• Project/Area Number: 24790437
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: The University of Tokyo
• Principal Investigator: HOSOYA Noriaki 東京大学, 医科学研究所, 特任助教 (30568928)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: HIV / 細胞指向性 / Envタンパク質 / ２重分割タンパク質 / 感染症 / ウイルス / 2重分割タンパク質

Research Abstract

We developed a novel HIV-1 phenotypic tropism assay (DSP-Pheno) based on the cell fusion. We employed dual split protein (DSP) composed of split green fluorescent protein (GFP) and split renilla luciferase (RL) as a marker for cell fusion. Equipped with two-way reporter system, RL and GFP, DSP-Pheno is a sensitive test with short turnaround time. Although maintenance of cell lines and laboratory equipment is necessary, it provides a safe assay system without infectious viruses. With further validation against other conventional analyses, DSP-Pheno may prove to be a useful laboratory tool.

Research Products

• [Journal Article] Development of a rapid cell-fusion-based phenotypic HIV-1 tropism assay. (2013)
  • Author(s): Teeranaipong P, Hosoya N, Kawana-Tachikawa A, Fujii T, Koibuchi T, Nakamura H, Koga M, Kondo N, Gao GF, Hoshino H, Matsuda Z, Iwamoto A.
  • Journal Title: Journal of the International AIDS Society. Volume: 16 Issue: 1 Pages: 18723-18732
  • DOI: 10.7448/ias.16.1.18723
  • Peer Reviewed
• [Journal Article] Significant Reductions in Gag-Protease-Mediated HIV-1 Replication Capacity during the Course of the Epidemic in Japan (2013)
  • Author(s): Nomura, S., N. Hosoya, Z. L. Brumme, M. A. Brockman, T. Kikuchi, M. Koga, H. Nakamura, T. Koibuchi, T. Fujii, J. M. Carlson, D. Heckerman, A. Kawana-Tachikawa, A. Iwamoto, and T. Miura.
  • Journal Title: J Virol Volume: 87 Issue: 3 Pages: 1465-76
  • DOI: 10.1128/jvi.02122-12
  • Peer Reviewed
• [Journal Article] Improved neutralizing antibody response in the second season after a single dose of pandemic (H1N1) 2009 influenza vaccine in HIV-1-positive adults (2012)
  • Author(s): Kikuchi T, Iwatsuki-Horimoto K, Adachi E, Koga M, Nakamura H, Hosoya N, Kawana-Tachikawa A, Koibuchi T, Miura T, Fujii T, Kawaoka Y, Iwamoto A.
  • Journal Title: Vaccine Volume: 30 Issue: 26 Pages: 3819-23
  • DOI: 10.1016/j.vaccine.2012.03.083
  • Peer Reviewed
• [Presentation] Phenotypic determination of HIV-1 envelope tropism using Dual Split Protein (DSP) mediated cell fusion assay system (2013)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Stucki Heinz, Yoshimi Kashi, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: Asian Research Forum on Emerging and Reemerging Infections–2013
  • Place of Presentation: 東京都文京区
• [Presentation] Phenotypic determination of HIV-1 envelope tropism using Dual Split Protein (DSP) mediated cell fusion assay system (2013)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Stucki Heinz, Yoshimi Kashi, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: 7th IAS Conference on HIV Pathogenesis, Treatment and Prevention
  • Place of Presentation: マレーシア、クアラルンプール
• [Presentation] 細胞膜融合と二重分割タンパク質(Dual Split Protein : DSP)を用いたHIV-1細胞指向性試験の開発 (2013)
  • Author(s): 細谷紀彰、Phairote Teeranaipong、立川(川名)愛、鯉渕智彦、古賀道子、中村仁美、近藤直幸、星野洪郎、松田前衛、岩本愛吉
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] Phenotypic determination of HIV-1 envelope tropism using Dual Split Protein (DSP) mediated cell fusion assay system. (2013)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Stucki Heinz, Yoshimi Kashi, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: 7th IAS Conference on HIV Pathogenesis Treatment and Prevention
  • Place of Presentation: クアラルンプール、マレーシア
• [Presentation] 細胞膜融合と二重分割タンパク質（Dual Split Protein: DSP）を用いたHIV-1細胞指向性試験の開発 (2013)
  • Author(s): 細谷 紀彰、Phairote Teeranaipong、立川（川名）愛、鯉渕 智彦、古賀 道子、中村 仁美、近藤 直幸、星野 洪郎、松田 前衛、岩本 愛吉
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] Phenotypic determination of HIV-1 envelope tropism using Dual Split Protein (DSP) mediated cell fusion assay system (2013)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Stucki Heinz, Yoshimi Kashi, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: Asian Research Forum on Emerging and Reemerging Infections - 2013
  • Place of Presentation: 東京都文京区
• [Presentation] Phenotypic determination of HIV-1 envelope tropism using Dual Split Protein (DSP) mediated cell fusion assay system. (2013)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Stucki Heinz, Yoshimi Kashi, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: 7th IAS Conference on HIV Pathogenesis, Treatment and Prevention
  • Place of Presentation: マレーシア、クアラルンプール
• [Presentation] Development of a HIV-1 Phenotypic Tropism Assay Using Dual Split Protein (DSP)-Mediated Membrane Fusion Detection System (2012)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: 1st Asian Conference on Hepatitis B and C, HIV and Influenza
  • Place of Presentation: 中国北京市
• [Presentation] A novel phenotypic HIV-1 tropism assay (2012)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: The 17th Chinese Biopharmaceutical Association Annual Conference
  • Place of Presentation: 中国青島市
• [Remarks]
  • URL: http://www.idimsut.jp
• [Remarks] 東京大学医科学研究所先端医療研究センター・感染症分野 附属病院・感染免疫内科
  • URL: http://www.idimsut.jp
• [Remarks] 東京大学医科学研究所 先端医療研究センター感染症分野 附属病院・感染免疫内科
  • URL: http://www.idimsut.jp