| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Aim of this study is to understand the chromosomal regulation for HIV expression. We planned to understand the spatial and chromosomal regulation of HIV provirus by using LacO/I system. To apply LacO/I system, Lac O repeat sequence must be inserted into HIV provirus in vivo. Therefore, we explored the potential of CRISPR/Cas9 system to edit HIV-1 genome as the first step. We found that CRISPR/Cas9 system efficiently cleaved and mutated HIV provirus in T cells, resulting in disturbing the expression. Furthermore, we established a new method that induces precise mutation in HIV provirus through HDR repair pathway using CRISPR/Cas9 system. It is suggested that these technologies enable to insert LacO repeat sequence into HIV provirus and have a potential to discover chromosomal regulation of HIV gene expression.
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