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Analysis for chromosomal regulation of HIV gene expression by proviral labeling

Research Project

Project/Area Number 24790438
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Virology
Research InstitutionKyoto University

Principal Investigator

EBINA Hirotaka  京都大学, ウイルス研究所, 助教 (60541951)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsHIV / 潜伏感染 / クロマチン / ゲノム編集 / HSV / ライブイメージング
Research Abstract

Aim of this study is to understand the chromosomal regulation for HIV expression. We planned to understand the spatial and chromosomal regulation of HIV provirus by using LacO/I system. To apply LacO/I system, Lac O repeat sequence must be inserted into HIV provirus in vivo. Therefore, we explored the potential of CRISPR/Cas9 system to edit HIV-1 genome as the first step. We found that CRISPR/Cas9 system efficiently cleaved and mutated HIV provirus in T cells, resulting in disturbing the expression. Furthermore, we established a new method that induces precise mutation in HIV provirus through HDR repair pathway using CRISPR/Cas9 system. It is suggested that these technologies enable to insert LacO repeat sequence into HIV provirus and have a potential to discover chromosomal regulation of HIV gene expression.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (29 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (18 results) Remarks (5 results)

  • [Journal Article] Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus2013

    • Author(s)
      Ebina H, Misawa N, Kanemura Y, Koyanagi Y
    • Journal Title

      Scientific Reports

      Volume: 3 Pages: 2510-2510

    • NAID

      120005324896

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus.2013

    • Author(s)
      Ebina H, Misawa N, Kanemura Y, Koyanagi Y.
    • Journal Title

      Sci. Rep.

      Volume: 3 Issue: 1 Pages: 2510-2510

    • DOI

      10.1038/srep02510

    • NAID

      120005324896

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integrase-independent HIV-1 infection is augmented under conditions of DNA damage and produces a viral reservoir2012

    • Author(s)
      Ebina H, Kanemura Y, Suzuki Y, Urata K, Misawa N, Koyanagi Y
    • Journal Title

      Virology

      Volume: 427(1) Pages: 44-50

    • NAID

      120004007474

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The hematopoietic cell-specific Rho GTPase inhibitor ARHGDIB/D4GDI limits HIV-1 replication2012

    • Author(s)
      Watanabe T, Urano E, Miyauchi K, Ichikawa R, Hamatake M, Misawa N, Sato K, Ebina H, Koyanagi Y, Komano J
    • Journal Title

      AIDS Res Hum Retroviruses

      Volume: 28(8) Pages: 913-22

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integrase-independent HIV-1 infection is augmented under conditions of DNA damage and produces a viral reservoir2012

    • Author(s)
      Ebina H, Kanemura Y, Suzuki Y, Urata K, Misawa N, Koyanagi Y
    • Journal Title

      Virology

      Volume: 427 Issue: 1 Pages: 44-50

    • DOI

      10.1016/j.virol.2012.02.004

    • NAID

      120004007474

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rho GTPase inhibitorARHGDIB/D4GDI limits HIV-1 replication2011

    • Author(s)
      Watanabe T, Urano E, Koyanagi Y, KomanoJ, 10人中9番目
    • Journal Title

      AIDS Research and Human Retroviruses

      Volume: (印刷中) Issue: 8 Pages: 1-10

    • DOI

      10.1089/aid.2011.0180

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of the CRISPR/Cas9 system editing for latent HIV-1 provirus2013

    • Author(s)
      蝦名博貴、三沢尚子、金村優香、小柳義夫
    • Organizer
      第36回日本分子生物学会年会
    • Related Report
      2013 Final Research Report
  • [Presentation] ゲノム編集技術による潜伏HIVプロウイルスの制御と除去2013

    • Author(s)
      蝦名博貴、三沢尚子、金村優香、小柳義夫
    • Organizer
      第27回日本エイズ学会学術集会
    • Related Report
      2013 Final Research Report
  • [Presentation] ゲノム編集技術による潜伏HIVプロウイルスの制御と除去2013

    • Author(s)
      蝦名博貴
    • Organizer
      第3回ゲノム編集研究会
    • Related Report
      2013 Final Research Report
  • [Presentation] I型IFN発現制御におけるSAMHD1の新規機能の解析2013

    • Author(s)
      金村優香、蝦名博貴、小柳義夫
    • Organizer
      第36回日本分子生物学会年会
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel function of SAMHD1 to involve in regulation of type I interferon induction.2013

    • Author(s)
      金村優香, Peter Gee, 蝦名博貴, Yoo Ji Seung, 藤田尚志, 小柳義夫.
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of the CRISPR/Cas9 system editing for latent HIV-1 provirus.2013

    • Author(s)
      Ebina, H., Misawa, N., Kanemura, Y., Koyanagi, Y.
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] ゲノム編集技術による潜伏HIVプロウイルスの制御と除去2013

    • Author(s)
      蝦名博貴, 三沢尚子, 金村優香, 小柳義夫.
    • Organizer
      第27回日本エイズ学会学術集会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] ゲノム編集技術による潜伏HIVプロウイルスの制御と除去2013

    • Author(s)
      蝦名博貴.
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNA damage induces illegitimate integration of HIV-1 in the presence of integrase inhibitor, leading to produce a viral reservoir2012

    • Author(s)
      Ebina H, Kanemura Y, Suzuki Y, Urata K, Misawa N, Koyanagi Y
    • Organizer
      37^<th> Retroviruses Meeting Cold Spring Harbor
    • Related Report
      2013 Final Research Report
  • [Presentation] インテグラーゼ非依存的なHIV cDNAの組込み機構2012

    • Author(s)
      蝦名博貴
    • Organizer
      第14回白馬シンポジウム
    • Related Report
      2013 Final Research Report
  • [Presentation] 生細胞におけるHIV cDNAの可視化とイメージング2012

    • Author(s)
      蝦名博貴、金村優香、小柳義夫
    • Organizer
      第35回日本分子生物学会年会
    • Related Report
      2013 Final Research Report
  • [Presentation] HIV cDNAの可視化とライブイメージングの確立2012

    • Author(s)
      蝦名博貴、金村優香、小柳義夫
    • Organizer
      第60回日本ウイルス学会学術集会
    • Related Report
      2013 Final Research Report
  • [Presentation] ウイルス感染により誘導されるIFNの発現制御とウイルス複製におけるSAMHD1の役割2012

    • Author(s)
      金村優香, Peter Gee,蝦名博貴,小柳義夫
    • Organizer
      第35回日本分子生物学年会
    • Place of Presentation
      福岡、4P-0447
    • Related Report
      2013 Final Research Report
  • [Presentation] DNA damage induces illegitimate integration of HIV-1 in the presence of integrase inhibitor, leading to produce a viral reservoir.

    • Author(s)
      Ebina H., Kanemura Y., Suzuki Y., Urata K., Misawa N. and Koyanagi Y.
    • Organizer
      37th Retroviruses Meeting Cold Spring Harbor
    • Place of Presentation
      New York (USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] インテ グラーゼ非依存的なHIV cDNAの組込み機構.

    • Author(s)
      蝦名博貴
    • Organizer
      第14回白馬シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] HIV cDNAの 可視化とライブイメージングの確立.

    • Author(s)
      蝦名博貴、金村優 香、小柳義夫.
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] 生細胞 におけるHIV cDNAの可視化とイメージング

    • Author(s)
      蝦名博貴、金村優 香、小柳義夫.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] ウイルス感 染により誘導されるIFNの発現制御とウイルス複製におけるSAMHD1の役割

    • Author(s)
      金村優香、Peter Gee、蝦名博貴、小柳義夫
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.virus.kyoto-u.ac.jp/virus/kansenbyoutai.html

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/KoyanagiHP/saito/TOP.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 京都大学 ウイルス研究所 附属ヒトレトロウイルス研究施設 ウイルス病態研究領域

    • URL

      http://www.virus.kyoto-u.ac.jp/virus/kansenbyoutai.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 京都大学 ウイルス研究所 ウイルス病態研究領域 小柳研究室

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/KoyanagiHP/saito/TOP.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 京都大学 ウイルス研究所 ウイルス病態研究領域 小柳研究室

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/KoyanagiHP/saito/TOP.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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