Analysis for chromosomal regulation of HIV gene expression by proviral labeling
| Project/Area Number |
24790438
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
EBINA Hirotaka 京都大学, ウイルス研究所, 助教 (60541951)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | HIV / 潜伏感染 / クロマチン / ゲノム編集 / HSV / ライブイメージング |
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| Research Abstract |
Aim of this study is to understand the chromosomal regulation for HIV expression. We planned to understand the spatial and chromosomal regulation of HIV provirus by using LacO/I system. To apply LacO/I system, Lac O repeat sequence must be inserted into HIV provirus in vivo. Therefore, we explored the potential of CRISPR/Cas9 system to edit HIV-1 genome as the first step. We found that CRISPR/Cas9 system efficiently cleaved and mutated HIV provirus in T cells, resulting in disturbing the expression. Furthermore, we established a new method that induces precise mutation in HIV provirus through HDR repair pathway using CRISPR/Cas9 system. It is suggested that these technologies enable to insert LacO repeat sequence into HIV provirus and have a potential to discover chromosomal regulation of HIV gene expression.
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Report
(3 results)
Research Products
(29 results)
