• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Understanding of viral replication through the interaction with coronavirus nsp1 and host proteins

Research Project

Project/Area Number 24790439
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Virology
Research InstitutionOsaka University

Principal Investigator

WATARU KAMITANI  大阪大学, 微生物病研究所, 特任准教授 (60551421)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsウイルス / RNA分解 / ウイルス複製 / RNA分解機構 / 感染症 / RNA / ヘリケース
Research Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease. Nsp1 binds to 40S ribosome subunits, which causes a translational shutoff in the cells expressing nsp1. The nsp1-40S complex further induces an endonucleolytic RNA cleavage near the 5'UTR of host mRNA, resulting in the promotion of RNA decay. However, the mechanism by which nsp1-induced RNA cleavage is unknown. We identified Upf1 as a binding partner of nsp1 by pulldown purification and mass spectrometry. Upf1 is a predominantly cytoplasmic RNA-binding protein that is essential for nonsense-mediated RNA decay pathway. Coimmunoprecipitation analyses in transfected cells confirmed a specific interaction between nsp1 and Upf1. Small interfering RNA-mediated knockdown of Upf1 resulted in suppression of nsp1-mediated RNA cleavage. Our data indicate a novel mechanism of host mRNA cleavage by SARA-CoV nsp1 protein.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] Severe acute respiratory syndrome coronavirus nsp1 facilitates efficient propagation in cells through a specific translational shutoff of host mRNA2012

    • Author(s)
      Tanaka T, Kamitani W, DeDiego ML, Enjuanes L, Matusura Y
    • Journal Title

      Journal of Virology

      Volume: 86(20) Issue: 20 Pages: 11128-11137

    • DOI

      10.1128/jvi.01700-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] SARS-CoVのnsp1タンパク質と相互作用する宿主因子の探索2012

    • Author(s)
      田中智久,松浦善治,神谷亘
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪、グランキューブ大阪
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] SARS coronavirus binds to the viral 5'UTR that induces host specific shutoff and mRNA degradation promotion2012

    • Author(s)
      田中智久,松浦善治,神谷亘
    • Organizer
      第34回内藤コンファレンス
    • Place of Presentation
      札幌、シャトレーゼガトーキングダムサッポロ
    • Related Report
      2013 Final Research Report
  • [Presentation] Circumvention of the translational shutoff in cells infected with SARS coronavirus through the interaction of nsp1 with 5'UTR of viral mRNA2012

    • Author(s)
      田中智久,松浦善治,神谷亘
    • Organizer
      The 11th Awaji International Forum of Infection and Immunity
    • Place of Presentation
      兵庫県、淡路夢舞台国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] SARS coronavirus binds to the viral 5’UTR that induces host specific shutoff and mRNA degradation promotion2012

    • Author(s)
      田中智久,松浦善治,神谷 亘
    • Organizer
      第34回内藤コンファレンス
    • Place of Presentation
      札幌、シャトレーゼ ガトーキングダム サッポロ
    • Related Report
      2012 Research-status Report
  • [Presentation] Circumvention of the translational shutoff in cells infected with SARS coronavirus through the interaction of nsp1 with 5’UTR of viral mRNA2012

    • Author(s)
      田中智久,松浦善治,神谷 亘
    • Organizer
      The 11th Awaji International Forum of Infection and Immunity
    • Place of Presentation
      兵庫県、淡路夢舞台国際会議場
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi