Elucidation of DNA replication, distribution and maintenance mechanisms of oncogenic virus by using cellular factors
| Project/Area Number |
24790441
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
OHSAKI Eriko 大阪大学, 医学(系)研究科(研究院), 助教 (50447801)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | カポジ肉腫関連ヘルペスウイルス / 潜伏感染 / DNA複製 / 核マトリックス / LANA / ori-P / ゲノム分配 |
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| Research Abstract |
Latency-associated nuclear antigen (LANA) recruits cellular DNA replication factors to the DNA origin (ori-P) in Kaposi's sarcoma-associated herpesvirus. To investigate the functional significance of the nuclear matrix in viral DNA replication, we constructed the chimeric protein of DBD fused to NuMA, which is one of the nuclear matrix proteins, and tested its DNA replication activity. Here we demonstrated that the NuMA-DBD localized in the nuclear matrix fraction, and it bound to the ori-P region, and showed high replication activity, which is similar to the full length of LANA. These results suggest that the recruitment of ori-P to the nuclear matrix is important for viral DNA replication.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Novel Monoclonal Antibodies for Identification of Multicentric Castleman' s Disease ; Kaposi' s Sarcoma-Associated Herpesvirus-Encoded vMIP-I and vMIP-II.2012
Author(s)
Nakano, K., Katano, H., Tadagaki, K., Sato, Y., Ohsaki, E., Mori, Y., Yamanishi, K., and Ueda, K.
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Journal Title
Virology
Volume: 425
Issue: 2
Pages: 95-102
DOI
Related Report
Peer Reviewed
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