| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
T-cell responses on murine rotavirus infection were analyzed in detail by using the mouse model. Murine rotavirus EW strain was inoculated orally to Balb/c mice. The maximum T-cell proliferation was observed at 10 days after infection. The nucleotide sequences of T-cell receptors (TCRs) expressed in ileum at 10 days after rotavirus infection were comprehensively analyzed by using next-generation sequencer. Then, it was found that rotavirus-specific TCRs accumulated on rotavirus-infected ileum and the sequences could be identified. In future studies, we will analyze the reactivity of these T-cells, which enables us to understand the mechanisms of protective immunity and cross-reactivity among virus strains.
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