Role of T-cells on protective immune response to rotavirus infection.

• Project/Area Number: 24790454
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: FUJII Yoshiki 国立感染症研究所, その他部局等, 研究員 (40518122)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ロタウイルス / マウス / T細胞受容体 / 次世代シーケンス / 動物実験 / Ｔ細胞 / 免疫応答

Outline of Final Research Achievements

T-cell responses on murine rotavirus infection were analyzed in detail by using the mouse model. Murine rotavirus EW strain was inoculated orally to Balb/c mice. The maximum T-cell proliferation was observed at 10 days after infection. The nucleotide sequences of T-cell receptors (TCRs) expressed in ileum at 10 days after rotavirus infection were comprehensively analyzed by using next-generation sequencer. Then, it was found that rotavirus-specific TCRs accumulated on rotavirus-infected ileum and the sequences could be identified. In future studies, we will analyze the reactivity of these T-cells, which enables us to understand the mechanisms of protective immunity and cross-reactivity among virus strains.

Research Products

• [Journal Article] Whole-genome sequence analysis of G3 and G14 equine group A rotaviruses isolated in the late 1990s and 2009-2010 (2015)
  • Author(s): Manabu Nemoto, Makoto Nagai, Hiroshi Tsunemitsu, Tsutomu Omatsu, Tetsuya Furuya, Junsuke Shirai, Takashi Kondo, Yoshiki Fujii, Reiko Todaka, Kazuhiko Katayama, Tetsuya Mizutani
  • Journal Title: Arch Virol. Volume: 160 Issue: 5 Pages: 1171-9
  • DOI: 10.1007/s00705-015-2374-6
  • Peer Reviewed
• [Journal Article] H2 genotypes of G4P[6], G5P[7], and G9[23] porcine rotaviruses show super-short RNA electropherotypes (2015)
  • Author(s): Makoto Nagai, Saya Shimada, Yoshiki Fujii, Hiromitsu Moriyama, Mami Oba, Yukie Katayama, Shinobu Tsuchiaka, Sachiko Okazaki, Tsutomu Omatsu, Tetsuya Furuya, Satoshi Koyama, Junsuke Shirai, Kazuhiko Katayama, Tetsuya Mizutani
  • Journal Title: Vet Microbiol. Volume: 176 Issue: 3-4 Pages: 250-6
  • DOI: 10.1016/j.vetmic.2015.02.002
  • Peer Reviewed
• [Journal Article] Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar. (2015)
  • Author(s): Ide T, Komoto S, Higo-Moriguchi K, Htun KW, Myint YY, Myat TW, Thant KZ, Thu HM, Win MM, Oo HN, Htut T, Wakuda M, Dennis FE, Haga K, Fujii Y, Katayama K, Rahman S, Nguyen SV, Umeda K, Oguma K, Tsuji T, Taniguchi K.
  • Journal Title: PLoS ONE Volume: 10 Issue: 5 Pages: 1-24
  • DOI: 10.1371/journal.pone.0124965
  • Peer Reviewed / Open Access
• [Journal Article] Spread and predominance in Japan of novel G1P[8] double-reassortant rotavirus strains possessing a DS-1-like genotype constellation typical of G2P[4] strains (2014)
  • Author(s): Yoshiki Fujii, Toyoko Nakagomi, Naoko Nishimura, Atsuko Noguchi, Sinobu Miura, Hisato Ito, Yen Hai Doan, Tsutomu Takahashi, Takao Ozaki, Kazuhiko Katayama, Osamu Nakagomi
  • Journal Title: Infect Genet Evol. Volume: 28 Pages: 426-33
  • DOI: 10.1016/j.meegid.2014.08.001
  • Peer Reviewed
• [Journal Article] Identification of Novel Ghanaian G8P[6] Human-Bovine Reassortant Rotavirus Strain by Next Generation Sequencing (2014)
  • Author(s): Francis E. Dennis, Yoshiki Fujii, Kei Haga, Susan Damanka, Belinda Lartey, Chantal A. Agbemabiese, Nobuo Ohta, George E. Armah, Kazuhiko Katayama
  • Journal Title: PLoS One Volume: 9 Issue: 6 Pages: e100699-e100699
  • DOI: 10.1371/journal.pone.0100699
  • Peer Reviewed / Open Access
• [Journal Article] Whole genomic analysis of porcine G10P[5] rotavirus strain P343 provides evidence for bovine-to-porcine interspecies transmission. (2014)
  • Author(s): Komoto S, Pongsuwanna Y, Ide T, Wakuda M, Guntapong R, Dennis FE, Haga K, Fujii Y, Katayama K, Taniguchi K.
  • Journal Title: Vet Microbiol Volume: 174 Issue: 3-4 Pages: 577-583
  • DOI: 10.1016/j.vetmic.2014.09.033
  • Peer Reviewed
• [Journal Article] Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer. (2014)
  • Author(s): Masuda T, Nagai M, Yamasato H, Tsuchiaka S, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Omatsu T, Furuya T, Koyama S, Shirai J, Taniguchi K, Fujii Y, Todaka R, Katayama K, Mizutani T.
  • Journal Title: Vet Microbiol. Volume: 171 Issue: 1-2 Pages: 66-73
  • DOI: 10.1016/j.vetmic.2014.03.009
  • Peer Reviewed / Open Access
• [Journal Article] Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing (2013)
  • Author(s): Minami-Fukuda Fujiko, Nagai Makoto, Takai Hikaru, Murakami Toshiaki, Ozawa Tadashi, Tsuchiaka Shinobu, Okazaki Sachiko, Katayama Yukie, Oba Mami, Nishiura Naomi, Sassa Yukiko, Omatsu Tsutomu, Furuya Tetsuya, Koyama Satoshi, Shirai Junsuke, Tsunemitsu Hiroshi, Fujii Yoshiki, Katayama Kazuhiko, Mizutani Tetsuya
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 12 Pages: 1651-1655
  • DOI: 10.1292/jvms.13-0265
  • NAID: 130003362271
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Immune-Related Gene Expression Profile in Laboratory Common Marmosets Assessed by an Accurate Quantitative Real-Time PCR Using Selected Reference Genes. (2013)
  • Author(s): Fujii Y
  • Journal Title: Pros ONE Volume: 8 Issue: 2 Pages: e56296-e56296
  • DOI: 10.1371/journal.pone.0056296
  • Peer Reviewed
• [Journal Article] A new method for quantitative analysis of the T cell receptor V region repertoires in healthy common marmosets by microplate hybridization assay. (2012)
  • Author(s): Kitaura K
  • Journal Title: J Immunol Methods Volume: 364 Issue: 1-2 Pages: 81-91
  • DOI: 10.1016/j.jim.2012.07.012
  • Peer Reviewed
• [Journal Article] Amplification of all 11 RNA segments of group A rotavirus based on reverse transcription polymerase chain reaction (2012)
  • Author(s): Yoshiki Fujii, Takashi Shimoike, Hirotaka Takagi, Kosuke Murakami, Reiko Todaka-Takai, YoungBin Park and Kazuhiko Katayama
  • Journal Title: Microbiol Immunol Volume: 56 Issue: 9 Pages: 630-638
  • DOI: 10.1111/j.1348-0421.2012.00479.x
  • Peer Reviewed
• [Presentation] ロタウイルスの検出法と疫学調査 (2015)
  • Author(s): 藤井克樹
  • Organizer: 平成26度希少感染症診断技術研修会
  • Place of Presentation: 東京
  • Year and Date: 2015-02-17 – 2015-02-18
  • Invited
• [Presentation] Continued circulation of novel G1P[8] double-reassortant strains carrying the DS-1-like genotype constellation in Japan. (2015)
  • Author(s): Yoshiki Fujii, Yen Hai Doan, Toyoko Nakagomi, Osamu Nakagomi, Kazuhiko Katayama
  • Organizer: 17th International Conference on Emerging Infectious Diseases (EID)
  • Place of Presentation: 台北
  • Year and Date: 2015-01-25 – 2015-01-29
• [Presentation] Molecular evolutional study of rotavirus genome using next generation sequencer. (2015)
  • Author(s): Yoshiki Fujii
  • Organizer: 感染症制御セミナー（NIID International Seminar on Infectious Diseases）
  • Place of Presentation: 東京
  • Year and Date: 2015-01-22 – 2015-01-23
  • Invited
• [Presentation] Impact of next generation sequencing technologies on whole genome analysis of rotaviruses (2014)
  • Author(s): 藤井克樹
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
  • Invited
• [Presentation] タイで検出されたG10P[5]ブタロタウイルスP343株ゲノムの全塩基配列の解析 (2014)
  • Author(s): 河本聡志、井手富彦、和久田光毅、Dennis Francis Ekow、芳賀慧、藤井克樹、片山和彦、谷口孝喜
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] ミャンマーにおけるG12P[8]ヒトロタウイルスの全塩基配列に基づく遺伝子解析 (2014)
  • Author(s): 井手富彦、河本聡志、森口匡子、Dennis Francis Ekow、芳賀慧、藤井克樹、片山和彦、Shofiqur Rahman、梅田浩二、Sa Van Nguyen、辻孝雄、谷口孝喜
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] ロタウイルスの分子疫学とロタウイルスワクチン (2014)
  • Author(s): 藤井克樹
  • Organizer: 第63回日本感染症学会東日本地方会総会学術集会・第61回日本化学療法学会東日本支部総会合同学会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-29 – 2014-10-31
  • Invited
• [Presentation] ゲノム遺伝子型構成解析による網羅的ロタウイルス分子疫学研究（2012年） (2013)
  • Author(s): 藤井克樹、下池貴志、戸高玲子、片山和彦
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 多様なA群ロタウイルス株に対応する感受性MA104細胞クローン樹立の試み (2013)
  • Author(s): 高木弘隆、藤井克樹、小林宣道、棚林清、片山和彦
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] カリシウイルスのリバースジェネティクスシステムを用いた感染性粒子の研究 (2013)
  • Author(s): 戸高玲子、村上耕介、岡智一郎、高木弘隆、朴英斌、下池貴志、藤井克樹、脇田隆字、中西章、片山和彦
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] ノロウイルスの小腸上皮細胞への結合メカニズム (2013)
  • Author(s): 村上耕介、戸高玲子、朴英斌、藤井克樹、下池貴志、脇田隆字、栗原千枝、穂刈量太、松田幹、片山和彦
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] マウスノロウイルス感染細胞内のウイルス蛋白質間とそのゲノムRNAとの相互作用 (2013)
  • Author(s): 下池貴志、高木弘隆、岡智一郎、村上耕介、戸高玲子、朴英斌、藤井克樹、脇田隆字、片山和彦
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] ロタウイルスの新知見 (2013)
  • Author(s): 藤井克樹
  • Organizer: ウイルス性下痢症研究会第25回学術集会
  • Place of Presentation: 神戸
• [Presentation] ロタウイルスの分子疫学 (2013)
  • Author(s): 藤井克樹
  • Organizer: 衛生微生物技術協議会第34回研究会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] ゲノム遺伝子型構成解析による網羅的ロタウイルス分子疫学基盤構築 (2013)
  • Author(s): 藤井克樹、村上耕介、戸高玲子、中込とよ子、中込治、片山和彦
  • Organizer: 第54回日本臨床ウイルス学会
  • Place of Presentation: 倉敷
• [Presentation] 我が国で流行したロタウイルスの遺伝子型の全国分布（2012年） (2013)
  • Author(s): 西村直子、野口篤子、伊藤陽里、辰巳正純、大場邦弘、中込治、中込とよ子、藤井克樹、片山和彦
  • Organizer: 第54回日本臨床ウイルス学会
  • Place of Presentation: 倉敷
• [Presentation] 京都府南丹地区におけるロタウイルス胃腸炎入院率 (2013)
  • Author(s): 伊藤陽里、中込とよ子、中込治、藤井克樹、片山和彦
  • Organizer: 第54回日本臨床ウイルス学会
  • Place of Presentation: 倉敷
• [Presentation] 秋田県由利地区におけるロタウイルス胃腸炎による入院率 (2013)
  • Author(s): 三浦忍、野口篤子、藤井克樹、中込治、片山和彦、中込とよ子、高橋勉
  • Organizer: 第54回日本臨床ウイルス学会
  • Place of Presentation: 倉敷
• [Presentation] ノロウイルス小腸上皮細胞への結合メカニズムの解析 (2013)
  • Author(s): 村上耕介、藤井克樹、戸高玲子、片山和彦
  • Organizer: 第54回日本臨床ウイルス学会
  • Place of Presentation: 倉敷
• [Presentation] RT-PCRによるA群ロタウイルスの全11セグメントの増幅法の構築 (2012)
  • Author(s): 藤井克樹、下池貴志、高木弘隆、Dennis Francis、村上耕介、朴英斌、戸高玲子、脇田隆字、片山和彦
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] ロタウイルスの新知見
  • Author(s): 藤井克樹
  • Organizer: ウイルス性下痢症研究会第24回学術集会
  • Place of Presentation: 大阪